Ficerafusp / 6azl

A4DAB117_6azl_D_C | Source PDB: 6azl | Chains: D, C | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6605
Max Score
2.1332
Min Score
-3.4988
Total Score
-282.02
Score CDR
-0.292
Score non-CDR
-0.705

Calculation Parameters

Chain Sequence(s) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSAASTKGPSVFPLAPSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKDILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4988
Maximum Score 2.1332
Average Score -0.6605
Total Score -282.0189

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q D -1.3693 Soluble
2 V D -0.6821 Soluble
3 Q D -1.2295 Soluble
4 L D 0.0000 Buried
5 K D -2.2256 Soluble
6 Q D 0.0000 Buried
7 S D -0.7547 Soluble
8 G D -1.0147 Soluble
9 P D -0.4647 Soluble
11 G D -0.4078 Soluble
12 L D -0.0921 Soluble
13 V D -0.8451 Soluble
14 Q D -1.7097 Soluble
15 P D -1.7293 Soluble
16 S D -1.7184 Soluble
17 Q D -2.1029 Soluble
18 S D -1.8591 Soluble
19 L D 0.0000 Buried
20 S D -0.7454 Soluble
21 I D 0.0000 Buried
22 T D -0.6224 Soluble
23 C D 0.0000 Buried
24 T D -1.1636 Soluble
25 V D 0.0000 Buried
26 S D -1.1705 Soluble
27 G D -0.9149 Soluble
28 F D -0.5304 Soluble
29 S D -0.9531 Soluble
30 L D 0.0000 Buried
35 T D -0.9591 Soluble
36 N D -0.9064 Soluble
37 Y D 0.3174 Aggregation-prone
38 G D 0.0000 Buried
39 V D 0.0000 Buried
40 H D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.4380 Soluble
45 S D -1.0199 Soluble
46 P D -1.1697 Soluble
47 G D -1.7315 Soluble
48 K D -2.2315 Soluble
49 G D -1.1363 Soluble
50 L D 0.0000 Buried
51 E D 0.0000 Buried
52 W D 0.0000 Buried
53 L D 0.0000 Buried
54 G D 0.0000 Buried
55 V D 0.0000 Buried
56 I D 0.0000 Buried
57 W D -0.5443 Soluble
58 S D -0.7009 Soluble
59 G D -1.2371 Soluble
63 G D -1.5126 Soluble
64 N D -1.8408 Soluble
65 T D -1.3016 Soluble
66 D D -1.0395 Soluble
67 Y D -0.4361 Soluble
68 N D -0.2046 Soluble
69 T D -0.2462 Soluble
70 P D -0.4064 Soluble
71 F D 0.0000 Buried
72 T D -0.5012 Soluble
74 S D -0.6922 Soluble
75 R D -1.2691 Soluble
76 L D 0.0000 Buried
77 S D -1.1882 Soluble
78 I D 0.0000 Buried
79 N D -1.8290 Soluble
80 K D -1.5189 Soluble
81 D D -1.8601 Soluble
82 N D -2.2845 Soluble
83 S D -1.7206 Soluble
84 K D -2.4105 Soluble
85 S D -1.6633 Soluble
86 Q D -1.6445 Soluble
87 V D 0.0000 Buried
88 F D -0.5880 Soluble
89 F D 0.0000 Buried
90 K D -1.6994 Soluble
91 M D 0.0000 Buried
92 N D -2.2843 Soluble
93 S D -1.7660 Soluble
94 L D 0.0000 Buried
95 Q D -2.0918 Soluble
96 S D -1.4943 Soluble
97 N D -2.0303 Soluble
98 D D 0.0000 Buried
99 T D -0.5769 Soluble
100 A D 0.0000 Buried
101 I D 0.5432 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 R D 0.0000 Buried
107 A D 0.0000 Buried
108 L D 1.0095 Aggregation-prone
109 T D 1.0941 Aggregation-prone
110 Y D 1.7915 Aggregation-prone
111 Y D 1.8667 Aggregation-prone
112 D D 0.9870 Aggregation-prone
113 Y D 0.5203 Aggregation-prone
114 E D 0.0000 Buried
115 F D 0.0000 Buried
116 A D 0.2683 Aggregation-prone
117 Y D 0.2820 Aggregation-prone
118 W D -0.4161 Soluble
119 G D 0.0000 Buried
120 Q D -1.7661 Soluble
121 G D -0.8844 Soluble
122 T D 0.0000 Buried
123 L D 0.2374 Aggregation-prone
124 V D 0.0000 Buried
125 T D -0.3329 Soluble
126 V D 0.0000 Buried
127 S D -0.7047 Soluble
128 A D -0.4060 Soluble
129 A D -0.3144 Soluble
130 S D -0.5123 Soluble
131 T D -0.5592 Soluble
132 K D -1.1722 Soluble
133 G D -1.3705 Soluble
134 P D -0.6024 Soluble
135 S D -0.2883 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.3708 Soluble
139 L D 0.0000 Buried
140 A D -1.2305 Soluble
141 P D 0.0000 Buried
142 S D -0.7167 Soluble
143 G D -0.7807 Soluble
144 G D -0.8704 Soluble
145 T D -0.6348 Soluble
146 A D 0.0000 Buried
147 A D 0.0000 Buried
148 L D 0.0000 Buried
149 G D 0.0000 Buried
150 C D 0.0000 Buried
151 L D 0.0000 Buried
152 V D 0.0000 Buried
153 K D 0.0000 Buried
154 D D -0.4434 Soluble
155 Y D 0.0000 Buried
156 F D 0.0000 Buried
157 P D 0.0000 Buried
158 E D -0.4567 Soluble
159 P D -0.6520 Soluble
160 V D -0.6594 Soluble
161 T D -0.5716 Soluble
162 V D -0.3203 Soluble
163 S D -0.3397 Soluble
164 W D 0.0000 Buried
165 N D -0.7356 Soluble
166 S D -0.6222 Soluble
167 G D -0.5508 Soluble
168 A D -0.2235 Soluble
169 L D 0.0149 Aggregation-prone
170 T D -0.1630 Soluble
171 S D -0.1936 Soluble
172 G D -0.2460 Soluble
173 V D 0.1454 Aggregation-prone
174 H D -0.2679 Soluble
175 T D 0.0244 Aggregation-prone
176 F D 0.0000 Buried
177 P D -0.2166 Soluble
178 A D 0.3223 Aggregation-prone
179 V D 0.6691 Aggregation-prone
180 L D 1.2353 Aggregation-prone
181 Q D 0.3480 Aggregation-prone
182 S D -0.0309 Soluble
183 S D -0.2127 Soluble
184 G D 0.0549 Aggregation-prone
185 L D 0.1628 Aggregation-prone
186 Y D 0.4427 Aggregation-prone
187 S D 0.0000 Buried
188 L D 0.0000 Buried
189 S D 0.0000 Buried
190 S D 0.0000 Buried
191 V D 0.0000 Buried
192 V D 0.0000 Buried
193 T D -0.1394 Soluble
194 V D 0.0000 Buried
195 P D -0.6398 Soluble
196 S D -0.6126 Soluble
197 S D -0.6033 Soluble
198 S D -0.6213 Soluble
199 L D -0.8532 Soluble
200 G D -0.9870 Soluble
201 T D -0.6733 Soluble
202 Q D -1.1402 Soluble
203 T D -1.1423 Soluble
204 Y D 0.0000 Buried
205 I D -1.3251 Soluble
206 C D 0.0000 Buried
207 N D -1.4167 Soluble
208 V D 0.0000 Buried
209 N D -1.8782 Soluble
210 H D 0.0000 Buried
211 K D -2.7899 Soluble
212 P D -1.6033 Soluble
213 S D -1.8094 Soluble
214 N D -2.5506 Soluble
215 T D -2.0553 Soluble
216 K D -2.6322 Soluble
217 V D -1.6127 Soluble
218 D D -2.3039 Soluble
219 K D -2.1948 Soluble
220 R D -2.6553 Soluble
221 V D 0.0000 Buried
222 E D -2.8980 Soluble
223 P D -1.9262 Soluble
224 K D -2.4533 Soluble
1 D C -1.3195 Soluble
2 I C 0.0000 Buried
3 L C 0.5969 Aggregation-prone
4 L C 0.0000 Buried
5 T C -0.3811 Soluble
6 Q C 0.0000 Buried
7 S C 0.1423 Aggregation-prone
8 P C 0.9427 Aggregation-prone
9 V C 2.1332 Aggregation-prone
10 I C 1.9023 Aggregation-prone
11 L C 0.8546 Aggregation-prone
12 S C -0.1445 Soluble
13 V C -1.1818 Soluble
14 S C -1.8634 Soluble
15 P C -1.9443 Soluble
16 G C -2.0287 Soluble
17 E C -2.8105 Soluble
18 R C -2.9704 Soluble
19 V C 0.0000 Buried
20 S C -0.9305 Soluble
21 F C 0.0000 Buried
22 S C -0.8837 Soluble
23 C C 0.0000 Buried
24 R C -2.4394 Soluble
25 A C 0.0000 Buried
26 S C -0.9689 Soluble
27 Q C -1.2935 Soluble
28 S C -1.4732 Soluble
29 I C 0.0000 Buried
36 G C -1.0271 Soluble
37 T C -0.7900 Soluble
38 N C -0.8125 Soluble
39 I C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C -1.1496 Soluble
45 R C -1.8234 Soluble
46 T C -1.6263 Soluble
47 N C -1.8182 Soluble
48 G C -1.5302 Soluble
49 S C -0.9870 Soluble
50 P C 0.0000 Buried
51 R C -0.6986 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 K C -0.8369 Soluble
56 Y C -0.6658 Soluble
57 A C 0.0000 Buried
65 S C -1.3194 Soluble
66 E C -2.0489 Soluble
67 S C -1.2124 Soluble
68 I C -0.5250 Soluble
69 S C -0.3158 Soluble
70 G C -0.4661 Soluble
71 I C -0.2477 Soluble
72 P C -0.3175 Soluble
74 S C -0.5176 Soluble
75 R C -1.0139 Soluble
76 F C 0.0000 Buried
77 S C -0.8970 Soluble
78 G C -0.7781 Soluble
79 S C -0.8740 Soluble
80 G C -1.0990 Soluble
83 S C -0.9603 Soluble
84 G C -1.3192 Soluble
85 T C -1.8424 Soluble
86 D C -2.4347 Soluble
87 F C 0.0000 Buried
88 T C -0.8916 Soluble
89 L C 0.0000 Buried
90 S C -0.8771 Soluble
91 I C 0.0000 Buried
92 N C -2.1131 Soluble
93 S C -2.1117 Soluble
94 V C 0.0000 Buried
95 E C -2.1615 Soluble
96 S C -2.1634 Soluble
97 E C -2.0681 Soluble
98 D C 0.0000 Buried
99 I C 0.0000 Buried
100 A C 0.0000 Buried
101 D C 0.0000 Buried
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 Q C 0.0000 Buried
106 Q C 0.0000 Buried
107 N C -0.6281 Soluble
108 N C -1.1298 Soluble
109 N C -0.9582 Soluble
114 W C 0.1433 Aggregation-prone
115 P C -0.1865 Soluble
116 T C 0.0000 Buried
117 T C -0.0261 Soluble
118 F C 0.1978 Aggregation-prone
119 G C 0.0000 Buried
120 A C 0.0674 Aggregation-prone
121 G C 0.2751 Aggregation-prone
122 T C 0.0000 Buried
123 K C 0.0311 Aggregation-prone
124 L C 0.0000 Buried
125 E C -0.7611 Soluble
126 L C 0.0000 Buried
127 K C -1.9413 Soluble
128 R C -1.1693 Soluble
129 T C 0.1179 Aggregation-prone
130 V C 1.0457 Aggregation-prone
131 A C 0.4628 Aggregation-prone
132 A C 0.0538 Aggregation-prone
133 P C 0.0000 Buried
134 S C -0.2201 Soluble
135 V C 0.0000 Buried
136 F C 0.1356 Aggregation-prone
137 I C 0.1450 Aggregation-prone
138 F C 0.0000 Buried
139 P C -0.5227 Soluble
140 P C -0.8576 Soluble
141 S C -1.9436 Soluble
142 D C -3.2208 Soluble
143 E C -3.2364 Soluble
144 Q C 0.0000 Buried
145 L C -2.3452 Soluble
146 K C -2.8644 Soluble
147 S C -1.7773 Soluble
148 G C -1.2931 Soluble
149 T C -0.8249 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C 0.0000 Buried
158 N C -0.8056 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.1734 Soluble
162 R C -1.8351 Soluble
163 E C -2.6801 Soluble
164 A C -1.9976 Soluble
165 K C -2.1973 Soluble
166 V C -1.0695 Soluble
167 Q C -0.7576 Soluble
168 W C 0.0000 Buried
169 K C -0.7512 Soluble
170 V C 0.0000 Buried
171 D C -2.0471 Soluble
172 N C -1.6384 Soluble
173 A C -0.4096 Soluble
174 L C 0.3704 Aggregation-prone
175 Q C -0.2655 Soluble
176 S C -0.4661 Soluble
177 G C -0.8665 Soluble
178 N C -0.7726 Soluble
179 S C -0.9842 Soluble
180 Q C -1.0882 Soluble
181 E C -1.5286 Soluble
182 S C -0.6463 Soluble
183 V C -0.5411 Soluble
184 T C -0.8380 Soluble
185 E C -1.8680 Soluble
186 Q C 0.0000 Buried
187 D C -1.8963 Soluble
188 S C -2.2340 Soluble
189 K C -2.5414 Soluble
190 D C -1.7556 Soluble
191 S C 0.0000 Buried
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.5701 Soluble
199 L C 0.0000 Buried
200 T C -0.3918 Soluble
201 L C -0.5950 Soluble
202 S C -1.1007 Soluble
203 K C -1.8483 Soluble
204 A C -1.7293 Soluble
205 D C -2.2513 Soluble
206 Y C 0.0000 Buried
207 E C -3.3162 Soluble
208 K C -3.4988 Soluble
209 H C -3.1617 Soluble
210 K C -3.2883 Soluble
211 V C -1.7055 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.9298 Soluble
216 V C 0.0000 Buried
217 T C -1.1148 Soluble
218 H C 0.0000 Buried
219 Q C -1.4892 Soluble
220 G C -0.2228 Soluble
221 L C -0.1581 Soluble
222 S C -0.4631 Soluble
223 S C -0.4387 Soluble
224 P C -0.5453 Soluble
225 V C -0.0672 Soluble
226 T C -0.4827 Soluble
227 K C -0.6079 Soluble
228 S C -0.6282 Soluble
229 F C -0.9425 Soluble
230 N C -2.1512 Soluble
231 R C -2.3987 Soluble
232 G C -1.5034 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.679 2.9306 CSV PDB
4.5 -0.7124 2.8783 CSV PDB
5.0 -0.7529 2.8066 CSV PDB
5.5 -0.792 2.7251 CSV PDB
6.0 -0.8194 2.6398 CSV PDB
6.5 -0.8268 2.5533 CSV PDB
7.0 -0.8135 2.4665 CSV PDB
7.5 -0.7861 2.3799 CSV PDB
8.0 -0.7511 2.3184 CSV PDB
8.5 -0.7113 2.3894 CSV PDB
9.0 -0.667 2.4882 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH