Timigutuzumab / 6mh2

A4DAB266_6mh2_B_A | Source PDB: 6mh2 | Chains: B, A | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6968
Max Score
2.2325
Min Score
-3.5245
Total Score
-294.05
Score CDR
-0.428
Score non-CDR
-0.7577

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWYAMDYWGQGTLVTVSSASTKGPSVFPLAPGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) B (Heavy), A (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5245
Maximum Score 2.2325
Average Score -0.6968
Total Score -294.0472

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E B -2.1488 Soluble
2 V B -1.4411 Soluble
3 Q B -1.3965 Soluble
4 L B 0.0000 Buried
5 V B 0.7786 Aggregation-prone
6 E B 0.0000 Buried
7 S B -0.4848 Soluble
8 G B -0.9811 Soluble
9 G B -0.5283 Soluble
11 G B -0.2417 Soluble
12 L B 0.0293 Aggregation-prone
13 V B 0.0000 Buried
14 Q B -1.6128 Soluble
15 P B -1.6475 Soluble
16 G B -1.3933 Soluble
17 G B -1.0240 Soluble
18 S B -1.1726 Soluble
19 L B -1.2521 Soluble
20 R B -2.2935 Soluble
21 L B 0.0000 Buried
22 S B -0.4879 Soluble
23 C B 0.0000 Buried
24 A B -0.1597 Soluble
25 A B 0.0000 Buried
26 S B -0.8865 Soluble
27 G B -1.3110 Soluble
28 F B -1.2022 Soluble
29 N B -1.9667 Soluble
30 I B 0.0000 Buried
35 K B -2.6440 Soluble
36 D B -2.5257 Soluble
37 T B 0.0000 Buried
38 Y B -0.1022 Soluble
39 I B 0.0000 Buried
40 H B 0.0000 Buried
41 W B 0.0000 Buried
42 V B 0.0000 Buried
43 R B 0.0000 Buried
44 Q B -0.8668 Soluble
45 A B -1.2082 Soluble
46 P B -1.0012 Soluble
47 G B -1.5411 Soluble
48 K B -2.4251 Soluble
49 G B -1.7166 Soluble
50 L B 0.0000 Buried
51 E B -1.3846 Soluble
52 W B 0.0000 Buried
53 V B 0.0000 Buried
54 A B 0.0000 Buried
55 R B -0.5255 Soluble
56 I B 0.0000 Buried
57 Y B -0.2748 Soluble
58 P B 0.0000 Buried
59 T B -1.1471 Soluble
62 N B -0.8387 Soluble
63 G B -0.2860 Soluble
64 Y B 0.5687 Aggregation-prone
65 T B -0.4215 Soluble
66 R B -1.9994 Soluble
67 Y B -1.9407 Soluble
68 A B -1.9073 Soluble
69 D B -2.7616 Soluble
70 S B -1.8061 Soluble
71 V B 0.0000 Buried
72 K B -2.9433 Soluble
74 G B -1.7932 Soluble
75 R B -1.6091 Soluble
76 F B 0.0000 Buried
77 T B -1.2655 Soluble
78 I B 0.0000 Buried
79 S B -0.2428 Soluble
80 A B -0.8601 Soluble
81 D B -1.6286 Soluble
82 T B -1.8388 Soluble
83 S B -1.5955 Soluble
84 K B -2.2239 Soluble
85 N B -1.7523 Soluble
86 T B -0.8873 Soluble
87 A B 0.0000 Buried
88 Y B -0.6929 Soluble
89 L B 0.0000 Buried
90 Q B -1.6542 Soluble
91 M B 0.0000 Buried
92 N B -1.3973 Soluble
93 S B -1.1901 Soluble
94 L B 0.0000 Buried
95 R B -2.4160 Soluble
96 A B -1.7621 Soluble
97 E B -2.3045 Soluble
98 D B 0.0000 Buried
99 T B -0.6717 Soluble
100 A B 0.0000 Buried
101 V B 0.1216 Aggregation-prone
102 Y B 0.0000 Buried
103 Y B 0.0000 Buried
104 C B 0.0000 Buried
105 S B 0.0000 Buried
106 R B -0.1172 Soluble
107 W B 0.2337 Aggregation-prone
108 Y B 1.3421 Aggregation-prone
114 A B 0.0000 Buried
115 M B 0.0000 Buried
116 D B -0.0600 Soluble
117 Y B -0.3406 Soluble
118 W B -0.2742 Soluble
119 G B 0.0000 Buried
120 Q B -1.3403 Soluble
121 G B -0.5717 Soluble
122 T B 0.0061 Aggregation-prone
123 L B 0.3213 Aggregation-prone
124 V B 0.0000 Buried
125 T B -0.3959 Soluble
126 V B 0.0000 Buried
127 S B -0.7959 Soluble
128 S B -0.7087 Soluble
129 A B -0.5244 Soluble
130 S B -0.6364 Soluble
131 T B -0.8846 Soluble
132 K B -1.5018 Soluble
133 G B -1.4732 Soluble
134 P B 0.0000 Buried
135 S B -0.5305 Soluble
136 V B 0.0000 Buried
137 F B 0.0000 Buried
138 P B -1.2903 Soluble
139 L B 0.0000 Buried
140 A B -0.6894 Soluble
141 P B -0.5108 Soluble
142 G B -0.6598 Soluble
143 T B -0.4628 Soluble
144 A B -0.2219 Soluble
145 A B 0.0000 Buried
146 L B 0.0000 Buried
147 G B 0.0000 Buried
148 C B 0.0000 Buried
149 L B 0.0000 Buried
150 V B 0.0000 Buried
151 K B 0.0000 Buried
152 D B -0.5145 Soluble
153 Y B 0.0000 Buried
154 F B 0.0000 Buried
155 P B 0.0000 Buried
156 E B -0.4947 Soluble
157 P B -0.6876 Soluble
158 V B -0.5581 Soluble
159 T B -0.5320 Soluble
160 V B -0.1071 Soluble
161 S B -0.3717 Soluble
162 W B 0.0000 Buried
163 N B -0.7374 Soluble
164 S B -0.6332 Soluble
165 G B -0.5160 Soluble
166 A B -0.2451 Soluble
167 L B -0.0318 Soluble
168 T B -0.1879 Soluble
169 S B -0.2119 Soluble
170 G B -0.3364 Soluble
171 V B 0.1459 Aggregation-prone
172 H B -0.3536 Soluble
173 T B -0.1023 Soluble
174 F B 0.0000 Buried
175 P B -0.2288 Soluble
176 A B 0.3434 Aggregation-prone
177 V B 0.6976 Aggregation-prone
178 L B 1.2593 Aggregation-prone
179 Q B 0.3589 Aggregation-prone
180 S B -0.0402 Soluble
181 S B -0.1956 Soluble
182 G B 0.1077 Aggregation-prone
183 L B 0.0636 Aggregation-prone
184 Y B 0.4619 Aggregation-prone
185 S B 0.3579 Aggregation-prone
186 L B 0.2098 Aggregation-prone
187 S B 0.0000 Buried
188 S B 0.0000 Buried
189 V B 0.0000 Buried
190 V B 0.0000 Buried
191 T B -0.1612 Soluble
192 V B 0.0000 Buried
193 P B -0.4921 Soluble
194 S B -0.5095 Soluble
195 S B -0.5242 Soluble
196 S B -0.4479 Soluble
197 L B -0.5470 Soluble
198 G B -0.8500 Soluble
199 T B -0.5074 Soluble
200 Q B -0.6239 Soluble
201 T B -0.8952 Soluble
202 Y B 0.0000 Buried
203 I B -1.2500 Soluble
204 C B 0.0000 Buried
205 N B -1.4292 Soluble
206 V B 0.0000 Buried
207 N B -1.8000 Soluble
208 H B 0.0000 Buried
209 K B -2.6887 Soluble
210 P B -1.5753 Soluble
211 S B -1.9170 Soluble
212 N B -2.5692 Soluble
213 T B -2.0829 Soluble
214 K B -2.6070 Soluble
215 V B -1.3715 Soluble
216 D B -2.5095 Soluble
217 K B -2.0195 Soluble
218 K B -2.5862 Soluble
219 V B 0.0000 Buried
220 E B -2.3921 Soluble
221 P B -1.1079 Soluble
1 D A -2.1229 Soluble
2 I A 0.0000 Buried
3 Q A -2.1650 Soluble
4 M A 0.0000 Buried
5 T A -1.3829 Soluble
6 Q A 0.0000 Buried
7 S A -0.7779 Soluble
8 P A -0.7153 Soluble
9 S A -0.9981 Soluble
10 S A -1.0000 Soluble
11 L A -0.7424 Soluble
12 S A -0.9672 Soluble
13 A A -0.9229 Soluble
14 S A -0.7388 Soluble
15 V A 0.1321 Aggregation-prone
16 G A -0.7098 Soluble
17 D A -1.7977 Soluble
18 R A -2.3671 Soluble
19 V A 0.0000 Buried
20 T A -0.6561 Soluble
21 I A 0.0000 Buried
22 T A -0.8928 Soluble
23 C A 0.0000 Buried
24 R A -2.9731 Soluble
25 A A 0.0000 Buried
26 S A -2.2717 Soluble
27 Q A -2.9137 Soluble
28 D A -2.9697 Soluble
29 V A 0.0000 Buried
36 N A -2.0406 Soluble
37 T A -1.0107 Soluble
38 A A 0.1325 Aggregation-prone
39 V A 0.0000 Buried
40 A A 0.0000 Buried
41 W A 0.0000 Buried
42 Y A 0.0000 Buried
43 Q A 0.0000 Buried
44 Q A 0.0000 Buried
45 K A -1.4285 Soluble
46 P A -1.4176 Soluble
47 G A -1.6082 Soluble
48 K A -2.4146 Soluble
49 A A -1.4808 Soluble
50 P A 0.0000 Buried
51 K A -1.1706 Soluble
52 L A 0.0000 Buried
53 L A 0.0000 Buried
54 I A 0.0000 Buried
55 Y A 1.4685 Aggregation-prone
56 S A 0.6198 Aggregation-prone
57 A A 0.0000 Buried
65 S A 0.5726 Aggregation-prone
66 F A 2.2325 Aggregation-prone
67 L A 1.5414 Aggregation-prone
68 Y A 0.7314 Aggregation-prone
69 S A -0.0074 Soluble
70 G A -0.4544 Soluble
71 V A 0.0000 Buried
72 P A -0.3268 Soluble
74 S A -0.3677 Soluble
75 R A -0.7252 Soluble
76 F A 0.0000 Buried
77 S A 0.0507 Aggregation-prone
78 G A -0.0865 Soluble
79 S A -1.0856 Soluble
80 R A -2.7023 Soluble
83 S A -1.7648 Soluble
84 G A -2.1799 Soluble
85 T A -2.5443 Soluble
86 D A -2.2891 Soluble
87 F A 0.0000 Buried
88 T A -1.0386 Soluble
89 L A 0.0000 Buried
90 T A -0.6367 Soluble
91 I A 0.0000 Buried
92 S A -1.4402 Soluble
93 S A -1.1276 Soluble
94 L A 0.0000 Buried
95 Q A -0.7168 Soluble
96 P A -1.2741 Soluble
97 E A -2.0382 Soluble
98 D A 0.0000 Buried
99 F A -1.1946 Soluble
100 A A 0.0000 Buried
101 T A -1.0459 Soluble
102 Y A 0.0000 Buried
103 Y A 0.0000 Buried
104 C A 0.0000 Buried
105 Q A 0.0000 Buried
106 Q A 0.0000 Buried
107 H A 0.5880 Aggregation-prone
108 Y A 0.4092 Aggregation-prone
109 T A 0.1941 Aggregation-prone
114 T A -0.5426 Soluble
115 P A -1.0065 Soluble
116 P A 0.0000 Buried
117 T A -0.6614 Soluble
118 F A -0.5322 Soluble
119 G A 0.0000 Buried
120 Q A -1.8123 Soluble
121 G A 0.0000 Buried
122 T A 0.0000 Buried
123 K A -1.9706 Soluble
124 V A 0.0000 Buried
125 E A -1.3228 Soluble
126 I A 0.0000 Buried
127 K A -1.1183 Soluble
128 R A -0.6166 Soluble
129 T A 0.2318 Aggregation-prone
130 V A 1.2052 Aggregation-prone
131 A A 0.4349 Aggregation-prone
132 A A 0.0199 Aggregation-prone
133 P A 0.0000 Buried
134 S A -0.1729 Soluble
135 V A 0.0000 Buried
136 F A 0.1687 Aggregation-prone
137 I A 0.1421 Aggregation-prone
138 F A 0.0000 Buried
139 P A -0.5049 Soluble
140 P A -0.8445 Soluble
141 S A -1.7252 Soluble
142 D A -3.0485 Soluble
143 E A -3.1600 Soluble
144 Q A 0.0000 Buried
145 L A -2.1231 Soluble
146 K A -2.7774 Soluble
147 S A -1.7558 Soluble
148 G A -1.1983 Soluble
149 T A -0.8455 Soluble
150 A A 0.0000 Buried
151 S A 0.0000 Buried
152 V A 0.0000 Buried
153 V A 0.0000 Buried
154 C A 0.0000 Buried
155 L A 0.0000 Buried
156 L A 0.0000 Buried
157 N A 0.0000 Buried
158 N A -0.9210 Soluble
159 F A 0.0000 Buried
160 Y A 0.0000 Buried
161 P A -1.1269 Soluble
162 R A -1.9176 Soluble
163 E A -2.7212 Soluble
164 A A -2.1069 Soluble
165 K A -2.3558 Soluble
166 V A 0.0000 Buried
167 Q A -0.7127 Soluble
168 W A 0.0000 Buried
169 K A -1.0031 Soluble
170 V A 0.0000 Buried
171 D A -2.3105 Soluble
172 N A -1.8000 Soluble
173 A A -0.4645 Soluble
174 L A 0.6512 Aggregation-prone
175 Q A -0.1389 Soluble
176 S A -0.3819 Soluble
177 G A -0.8490 Soluble
178 N A -0.7021 Soluble
179 S A -1.0735 Soluble
180 Q A -1.2116 Soluble
181 E A -1.8355 Soluble
182 S A -0.6562 Soluble
183 V A -0.4608 Soluble
184 T A -0.9714 Soluble
185 E A -2.0734 Soluble
186 Q A 0.0000 Buried
187 D A -2.0277 Soluble
188 S A -2.1161 Soluble
189 K A -2.4652 Soluble
190 D A -1.6773 Soluble
191 S A 0.0000 Buried
192 T A 0.0000 Buried
193 Y A 0.0000 Buried
194 S A 0.0000 Buried
195 L A 0.0000 Buried
196 S A 0.0000 Buried
197 S A 0.0000 Buried
198 T A -0.6011 Soluble
199 L A 0.0000 Buried
200 T A -0.4090 Soluble
201 L A -0.7116 Soluble
202 S A -1.1533 Soluble
203 K A -2.0322 Soluble
204 A A -1.9266 Soluble
205 D A -2.8118 Soluble
206 Y A 0.0000 Buried
207 E A -3.0842 Soluble
208 K A -3.5245 Soluble
209 H A -3.0314 Soluble
210 K A -2.9174 Soluble
211 V A -1.4210 Soluble
212 Y A 0.0000 Buried
213 A A 0.0000 Buried
214 C A 0.0000 Buried
215 E A -0.8440 Soluble
216 V A 0.0000 Buried
217 T A -1.2235 Soluble
218 H A 0.0000 Buried
219 Q A -1.5510 Soluble
220 G A -0.2949 Soluble
221 L A -0.1218 Soluble
222 S A -0.4439 Soluble
223 S A -0.3846 Soluble
224 P A -0.5059 Soluble
225 V A 0.0248 Aggregation-prone
226 T A -0.5292 Soluble
227 K A -0.8069 Soluble
228 S A -0.6896 Soluble
229 F A -0.9467 Soluble
230 N A -2.0394 Soluble
231 R A -2.4036 Soluble
232 G A -2.1506 Soluble
233 E A -2.2157 Soluble
234 C A -0.4808 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6978 3.9072 CSV PDB
4.5 -0.7443 3.9072 CSV PDB
5.0 -0.7989 3.9072 CSV PDB
5.5 -0.8517 3.9072 CSV PDB
6.0 -0.8917 3.9072 CSV PDB
6.5 -0.9103 3.9072 CSV PDB
7.0 -0.9062 3.9072 CSV PDB
7.5 -0.8859 3.9072 CSV PDB
8.0 -0.8561 3.9071 CSV PDB
8.5 -0.8186 3.9069 CSV PDB
9.0 -0.7732 3.9064 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH