Ansuvimab / 5fha

A4DAB020_5fha_H_L | Source PDB: 5fha | Chains: H, L | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6853
Max Score
1.1677
Min Score
-3.3335
Total Score
-292.62
Score CDR
-0.7115
Score non-CDR
-0.6821

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLIQPGGSLRLSCAASGFALRMYDMHWVRQTIDKRLEWVSAVGPSGDTYYADSVKGRFAVSRENAKNSLSLQMNSLTAGDTAIYYCVRSDRGVAGLFDSWGQGILVTVSSASTKGPSVFPLAPSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQMTQSPSSLSASVGDRITITCRASQAFDNYVAWYQQRPGKVPKLLISAASALHAGVPSRFSGSGSGTHFTLTISSLQPEDVATYYCQNYNSAPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3335
Maximum Score 1.1677
Average Score -0.6853
Total Score -292.6225

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.1830 Soluble
2 V H -1.2489 Soluble
3 Q H -1.4087 Soluble
4 L H 0.0000 Buried
5 V H 1.1677 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.3363 Soluble
8 G H -0.9918 Soluble
9 G H -0.4621 Soluble
11 G H -0.0075 Soluble
12 L H 0.1414 Aggregation-prone
13 I H -0.1769 Soluble
14 Q H -1.0872 Soluble
15 P H -0.9846 Soluble
16 G H -1.0022 Soluble
17 G H -0.7807 Soluble
18 S H -1.0878 Soluble
19 L H -1.2127 Soluble
20 R H -2.2962 Soluble
21 L H 0.0000 Buried
22 S H -0.5284 Soluble
23 C H 0.0000 Buried
24 A H 0.0222 Aggregation-prone
25 A H 0.0000 Buried
26 S H -1.0195 Soluble
27 G H -1.3845 Soluble
28 F H -0.7153 Soluble
29 A H -0.7935 Soluble
30 L H 0.0000 Buried
35 R H -2.1185 Soluble
36 M H -1.1418 Soluble
37 Y H -1.3325 Soluble
38 D H -1.6410 Soluble
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H 0.0000 Buried
45 T H -0.3484 Soluble
46 I H 0.4414 Aggregation-prone
47 D H -1.9438 Soluble
48 K H -2.3009 Soluble
49 R H -2.0053 Soluble
50 L H 0.0000 Buried
51 E H -0.7114 Soluble
52 W H -0.1924 Soluble
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 A H 0.0000 Buried
56 V H 0.0000 Buried
57 G H 0.0000 Buried
58 P H -1.5107 Soluble
59 S H -1.2611 Soluble
63 G H -1.3186 Soluble
64 D H -1.0068 Soluble
65 T H -0.0471 Soluble
66 Y H 0.7226 Aggregation-prone
67 Y H -0.3242 Soluble
68 A H -1.0925 Soluble
69 D H -2.3763 Soluble
70 S H -1.6353 Soluble
71 V H 0.0000 Buried
72 K H -2.4365 Soluble
74 G H -1.7764 Soluble
75 R H -1.5849 Soluble
76 F H 0.0000 Buried
77 A H -0.7808 Soluble
78 V H 0.0000 Buried
79 S H -0.9024 Soluble
80 R H -1.5992 Soluble
81 E H -2.1502 Soluble
82 N H -2.2372 Soluble
83 A H -1.6794 Soluble
84 K H -2.2758 Soluble
85 N H -1.8782 Soluble
86 S H -1.0434 Soluble
87 L H 0.0000 Buried
88 S H -0.9637 Soluble
89 L H 0.0000 Buried
90 Q H -1.6048 Soluble
91 M H 0.0000 Buried
92 N H -1.1761 Soluble
93 S H -0.8792 Soluble
94 L H 0.0000 Buried
95 T H -0.5846 Soluble
96 A H -0.4155 Soluble
97 G H -0.1853 Soluble
98 D H 0.0000 Buried
99 T H 0.5113 Aggregation-prone
100 A H 0.0000 Buried
101 I H 0.7333 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 V H 0.0000 Buried
106 R H -0.9269 Soluble
107 S H 0.0000 Buried
108 D H -1.6804 Soluble
109 R H -2.2925 Soluble
110 G H -1.3723 Soluble
111 V H -0.7453 Soluble
112 A H -0.6318 Soluble
113 G H 0.0000 Buried
114 L H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -1.2787 Soluble
117 S H -0.7649 Soluble
118 W H -0.5076 Soluble
119 G H 0.0000 Buried
120 Q H -0.9848 Soluble
121 G H -0.1322 Soluble
122 I H 0.4004 Aggregation-prone
123 L H 0.5061 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.5039 Soluble
128 S H -0.6225 Soluble
129 A H -0.4371 Soluble
130 S H -0.5136 Soluble
131 T H -0.6612 Soluble
132 K H -1.1317 Soluble
133 G H -1.3821 Soluble
134 P H 0.0000 Buried
135 S H -0.4400 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0944 Soluble
139 L H 0.0000 Buried
140 A H -1.2089 Soluble
141 P H 0.0000 Buried
142 S H -0.9067 Soluble
143 G H -0.8211 Soluble
144 G H -0.8656 Soluble
145 T H -0.6075 Soluble
146 A H 0.0000 Buried
147 A H 0.0000 Buried
148 L H 0.0000 Buried
149 G H 0.0000 Buried
150 C H 0.0000 Buried
151 L H 0.0000 Buried
152 V H 0.0000 Buried
153 K H 0.0000 Buried
154 D H -0.3331 Soluble
155 Y H 0.0000 Buried
156 F H 0.0000 Buried
157 P H 0.0000 Buried
158 E H -0.4730 Soluble
159 P H -0.6984 Soluble
160 V H -0.6941 Soluble
161 T H -0.6123 Soluble
162 V H -0.3041 Soluble
163 S H -0.4507 Soluble
164 W H 0.0000 Buried
165 N H -0.7153 Soluble
166 S H -0.7028 Soluble
167 G H -0.5969 Soluble
168 A H -0.2746 Soluble
169 L H -0.0399 Soluble
170 T H -0.1944 Soluble
171 S H -0.1945 Soluble
172 G H -0.2345 Soluble
173 V H 0.1518 Aggregation-prone
174 H H -0.2544 Soluble
175 T H -0.1198 Soluble
176 F H 0.0000 Buried
177 P H -0.1456 Soluble
178 A H 0.3314 Aggregation-prone
179 V H 0.6353 Aggregation-prone
180 L H 1.0973 Aggregation-prone
181 Q H 0.2270 Aggregation-prone
182 S H -0.1063 Soluble
183 S H -0.2044 Soluble
184 G H 0.0496 Aggregation-prone
185 L H 0.1557 Aggregation-prone
186 Y H 0.4260 Aggregation-prone
187 S H 0.0000 Buried
188 L H 0.0000 Buried
189 S H 0.0000 Buried
190 S H 0.0000 Buried
191 V H 0.0000 Buried
192 V H 0.0000 Buried
193 T H -0.1455 Soluble
194 V H 0.0000 Buried
195 P H -0.5468 Soluble
196 S H -0.5919 Soluble
197 S H -0.6325 Soluble
198 S H -0.5819 Soluble
199 L H -0.9023 Soluble
200 G H -1.2036 Soluble
201 T H -0.6959 Soluble
202 Q H -1.2164 Soluble
203 T H -1.0294 Soluble
204 Y H 0.0000 Buried
205 I H -1.0491 Soluble
206 C H 0.0000 Buried
207 N H -1.5597 Soluble
208 V H 0.0000 Buried
209 N H -2.1315 Soluble
210 H H 0.0000 Buried
211 K H -2.7634 Soluble
212 P H -1.5930 Soluble
213 S H -1.8313 Soluble
214 N H -2.6128 Soluble
215 T H -2.1223 Soluble
216 K H -2.8193 Soluble
217 V H -1.7197 Soluble
218 D H -2.5600 Soluble
219 K H -2.0799 Soluble
220 K H -2.2298 Soluble
221 V H 0.0000 Buried
222 E H -2.7403 Soluble
223 P H -1.9924 Soluble
224 K H -2.2530 Soluble
1 D L -0.8487 Soluble
2 I L 0.3572 Aggregation-prone
3 Q L -1.1641 Soluble
4 M L 0.0000 Buried
5 T L -1.2786 Soluble
6 Q L -0.9539 Soluble
7 S L -0.8415 Soluble
8 P L -0.5501 Soluble
9 S L -0.7050 Soluble
10 S L -0.5927 Soluble
11 L L -0.3860 Soluble
12 S L -0.5695 Soluble
13 A L 0.0000 Buried
14 S L -0.7815 Soluble
15 V L 0.2372 Aggregation-prone
16 G L -0.7649 Soluble
17 D L -1.9394 Soluble
18 R L -2.3980 Soluble
19 I L 0.0000 Buried
20 T L -0.5153 Soluble
21 I L 0.0000 Buried
22 T L -0.8348 Soluble
23 C L 0.0000 Buried
24 R L -2.6630 Soluble
25 A L -1.8549 Soluble
26 S L -1.3835 Soluble
27 Q L -1.7819 Soluble
28 A L -1.1726 Soluble
29 F L 0.0000 Buried
36 D L -2.1615 Soluble
37 N L -1.4744 Soluble
38 Y L -0.4837 Soluble
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.4860 Soluble
45 R L -2.1089 Soluble
46 P L -1.7453 Soluble
47 G L -1.7454 Soluble
48 K L -2.5045 Soluble
49 V L -1.4736 Soluble
50 P L 0.0000 Buried
51 K L -1.8708 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 S L 0.0000 Buried
56 A L -0.1125 Soluble
57 A L 0.0000 Buried
65 S L -0.2188 Soluble
66 A L 0.1103 Aggregation-prone
67 L L 0.3379 Aggregation-prone
68 H L -0.2598 Soluble
69 A L -0.1757 Soluble
70 G L -0.3671 Soluble
71 V L -0.2478 Soluble
72 P L -0.2809 Soluble
74 S L -0.3574 Soluble
75 R L -0.7597 Soluble
76 F L 0.0000 Buried
77 S L -0.2915 Soluble
78 G L -0.2762 Soluble
79 S L -0.6642 Soluble
80 G L -0.8695 Soluble
83 S L -1.1140 Soluble
84 G L -1.4873 Soluble
85 T L -1.8763 Soluble
86 H L -1.9686 Soluble
87 F L 0.0000 Buried
88 T L -0.7524 Soluble
89 L L 0.0000 Buried
90 T L -0.6168 Soluble
91 I L 0.0000 Buried
92 S L -1.4594 Soluble
93 S L -1.1270 Soluble
94 L L 0.0000 Buried
95 Q L -0.9409 Soluble
96 P L -1.2624 Soluble
97 E L -2.0037 Soluble
98 D L 0.0000 Buried
99 V L -1.0846 Soluble
100 A L 0.0000 Buried
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 N L 0.0000 Buried
107 Y L 0.0000 Buried
108 N L -0.7915 Soluble
109 S L -0.3609 Soluble
114 A L -0.0334 Soluble
115 P L -0.1904 Soluble
116 L L 0.0171 Aggregation-prone
117 T L 0.0000 Buried
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -1.4526 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.2779 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L 0.0000 Buried
127 K L -1.2331 Soluble
128 R L -0.8988 Soluble
129 T L -0.1738 Soluble
130 V L 0.3372 Aggregation-prone
131 A L 0.0002 Aggregation-prone
132 A L -0.1175 Soluble
133 P L 0.0000 Buried
134 S L -0.1598 Soluble
135 V L 0.0000 Buried
136 F L 0.1632 Aggregation-prone
137 I L 0.1733 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.7390 Soluble
140 P L -0.8332 Soluble
141 S L -1.8324 Soluble
142 D L -2.9670 Soluble
143 E L -3.0256 Soluble
144 Q L 0.0000 Buried
145 L L -2.2829 Soluble
146 K L -2.8681 Soluble
147 S L -1.8001 Soluble
148 G L -1.3983 Soluble
149 T L -1.1046 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.7593 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.2871 Soluble
162 R L -1.8915 Soluble
163 E L -2.6863 Soluble
164 A L -2.1417 Soluble
165 K L -2.4300 Soluble
166 V L 0.0000 Buried
167 Q L -0.8039 Soluble
168 W L 0.0000 Buried
169 K L -0.7389 Soluble
170 V L 0.0000 Buried
171 D L -1.7400 Soluble
172 N L -1.4289 Soluble
173 A L -0.3925 Soluble
174 L L 0.5101 Aggregation-prone
175 Q L -0.2257 Soluble
176 S L -0.5183 Soluble
177 G L -0.8836 Soluble
178 N L -0.8263 Soluble
179 S L -0.9991 Soluble
180 Q L -1.1000 Soluble
181 E L -1.2693 Soluble
182 S L -0.3179 Soluble
183 V L -0.1232 Soluble
184 T L -0.8449 Soluble
185 E L -2.0554 Soluble
186 Q L -1.6997 Soluble
187 D L -1.9857 Soluble
188 S L -1.9798 Soluble
189 K L -2.2604 Soluble
190 D L -1.7203 Soluble
191 S L -1.7603 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5084 Soluble
199 L L 0.0000 Buried
200 T L -0.4638 Soluble
201 L L -0.7082 Soluble
202 S L -1.2787 Soluble
203 K L -2.0959 Soluble
204 A L -1.9129 Soluble
205 D L -2.6715 Soluble
206 Y L 0.0000 Buried
207 E L -3.0982 Soluble
208 K L -3.3335 Soluble
209 H L -2.7028 Soluble
210 K L -2.6163 Soluble
211 V L -1.4325 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.9996 Soluble
216 V L 0.0000 Buried
217 T L -1.3043 Soluble
218 H L 0.0000 Buried
219 Q L -1.6428 Soluble
220 G L -0.4925 Soluble
221 L L -0.3093 Soluble
222 S L -0.5047 Soluble
223 S L -0.4510 Soluble
224 P L -0.6898 Soluble
225 V L -0.0889 Soluble
226 T L -0.4798 Soluble
227 K L -0.5830 Soluble
228 S L -0.5432 Soluble
229 F L -0.7966 Soluble
230 N L -1.8977 Soluble
231 R L -2.0337 Soluble
232 G L -1.2912 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7052 2.3594 CSV PDB
4.5 -0.7465 2.2986 CSV PDB
5.0 -0.7931 2.2341 CSV PDB
5.5 -0.836 2.1684 CSV PDB
6.0 -0.8656 2.1023 CSV PDB
6.5 -0.8746 2.0363 CSV PDB
7.0 -0.8634 1.971 CSV PDB
7.5 -0.8389 1.9081 CSV PDB
8.0 -0.8064 1.8513 CSV PDB
8.5 -0.7676 1.8076 CSV PDB
9.0 -0.7223 1.7817 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH