Bafisontamab / 5sx5

A4DAB031_5sx5_J_K | Source PDB: 5sx5 | Chains: J, K | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.674
Max Score
1.4055
Min Score
-3.8872
Total Score
-283.09
Score CDR
-0.4715
Score non-CDR
-0.6989

Calculation Parameters

Chain Sequence(s) QVQLQESGPGLVKPSETLSLTCTVSGGSVSSGDYYWTWIRQSPGKGLEWIGHIYYSGNTNYNPSLKSRLTISIDTSKTQFSLKLSSVTAADTAIYYCVRDRVTGAFDIWGQGTMVTVSSASTKGPSVFPLAPCSSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPQTYTCNVDHKPSNTKVDKTVEDIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYDASNLETGVPSRFSGSGSGTDFTFTISSLQPEDIATYFCQHFDHLPLAFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) J (Heavy), K (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8872
Maximum Score 1.4055
Average Score -0.674
Total Score -283.0917

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q J -1.5719 Soluble
2 V J -1.2748 Soluble
3 Q J -1.9201 Soluble
4 L J 0.0000 Buried
5 Q J -1.7031 Soluble
6 E J 0.0000 Buried
7 S J -0.6441 Soluble
8 G J -0.6279 Soluble
9 P J -0.5997 Soluble
11 G J -0.3957 Soluble
12 L J -0.3178 Soluble
13 V J 0.0000 Buried
14 K J -2.0702 Soluble
15 P J -1.4787 Soluble
16 S J -1.4672 Soluble
17 E J -2.1606 Soluble
18 T J -1.4725 Soluble
19 L J 0.0000 Buried
20 S J -0.9278 Soluble
21 L J 0.0000 Buried
22 T J -0.6325 Soluble
23 C J 0.0000 Buried
24 T J -1.0849 Soluble
25 V J 0.0000 Buried
26 S J -1.4147 Soluble
27 G J -1.2809 Soluble
28 G J -1.0326 Soluble
29 S J -0.8166 Soluble
30 V J 0.0000 Buried
31 S J -0.3177 Soluble
34 S J -0.4977 Soluble
35 G J -0.5539 Soluble
36 D J -1.2908 Soluble
37 Y J -0.1070 Soluble
38 Y J 0.4627 Aggregation-prone
39 W J 0.0000 Buried
40 T J 0.0000 Buried
41 W J 0.0000 Buried
42 I J 0.0000 Buried
43 R J 0.0000 Buried
44 Q J -0.4578 Soluble
45 S J -0.6436 Soluble
46 P J -0.8863 Soluble
47 G J -1.2589 Soluble
48 K J -1.6658 Soluble
49 G J -1.2022 Soluble
50 L J 0.0000 Buried
51 E J -0.6112 Soluble
52 W J 0.0000 Buried
53 I J 0.0000 Buried
54 G J 0.0000 Buried
55 H J 0.0000 Buried
56 I J 0.0000 Buried
57 Y J 0.0608 Aggregation-prone
58 Y J 0.3469 Aggregation-prone
59 S J -0.2774 Soluble
63 G J -0.7419 Soluble
64 N J -1.4181 Soluble
65 T J -0.7902 Soluble
66 N J -0.7333 Soluble
67 Y J -0.6310 Soluble
68 N J -0.6604 Soluble
69 P J -0.9609 Soluble
70 S J -0.8706 Soluble
71 L J 0.0000 Buried
72 K J -1.8921 Soluble
74 S J -1.2255 Soluble
75 R J -1.2454 Soluble
76 L J 0.0000 Buried
77 T J -0.8331 Soluble
78 I J 0.0000 Buried
79 S J -0.4247 Soluble
80 I J -0.2720 Soluble
81 D J -1.1740 Soluble
82 T J -0.9600 Soluble
83 S J -1.3499 Soluble
84 K J -2.1151 Soluble
85 T J -1.4063 Soluble
86 Q J -1.2182 Soluble
87 F J 0.0000 Buried
88 S J -0.5292 Soluble
89 L J 0.0000 Buried
90 K J -1.1714 Soluble
91 L J 0.0000 Buried
92 S J -1.1239 Soluble
93 S J -1.1973 Soluble
94 V J 0.0000 Buried
95 T J -0.6472 Soluble
96 A J -0.2080 Soluble
97 A J -0.2025 Soluble
98 D J 0.0000 Buried
99 T J 0.1271 Aggregation-prone
100 A J 0.0000 Buried
101 I J 0.4154 Aggregation-prone
102 Y J 0.0000 Buried
103 Y J 0.0000 Buried
104 C J 0.0000 Buried
105 V J 0.0000 Buried
106 R J 0.0000 Buried
107 D J 0.0000 Buried
108 R J -0.0880 Soluble
109 V J 0.5519 Aggregation-prone
110 T J 0.1643 Aggregation-prone
113 G J 0.0000 Buried
114 A J 0.0000 Buried
115 F J 0.0000 Buried
116 D J -0.4717 Soluble
117 I J -0.5929 Soluble
118 W J -0.9148 Soluble
119 G J 0.0000 Buried
120 Q J -1.9611 Soluble
121 G J -0.9747 Soluble
122 T J -0.3246 Soluble
123 M J 0.2645 Aggregation-prone
124 V J 0.0000 Buried
125 T J 0.0000 Buried
126 V J 0.0000 Buried
127 S J -0.6472 Soluble
128 S J -0.8054 Soluble
129 A J -0.5136 Soluble
130 S J -0.6088 Soluble
131 T J -0.5667 Soluble
132 K J -1.0897 Soluble
133 G J -1.2998 Soluble
134 P J -0.5599 Soluble
135 S J -0.4305 Soluble
136 V J 0.0000 Buried
137 F J 0.0000 Buried
138 P J -0.8887 Soluble
139 L J 0.0000 Buried
140 A J -0.4010 Soluble
141 P J -0.2546 Soluble
142 C J -0.5334 Soluble
143 S J -0.4461 Soluble
144 S J -0.2825 Soluble
145 T J -0.2039 Soluble
146 A J -0.0350 Soluble
147 A J 0.0000 Buried
148 L J -0.0121 Soluble
149 G J 0.0000 Buried
150 C J 0.0000 Buried
151 L J 0.0000 Buried
152 V J 0.0000 Buried
153 K J 0.0000 Buried
154 D J -0.3294 Soluble
155 Y J 0.0000 Buried
156 F J 0.0000 Buried
157 P J 0.0000 Buried
158 E J -0.3337 Soluble
159 P J -0.5317 Soluble
160 V J -0.5614 Soluble
161 T J -0.5135 Soluble
162 V J -0.3160 Soluble
163 S J -0.4818 Soluble
164 W J 0.0000 Buried
165 N J -0.8431 Soluble
166 S J -0.8110 Soluble
167 G J -0.5881 Soluble
168 A J -0.2801 Soluble
169 L J -0.0671 Soluble
170 T J -0.1939 Soluble
171 S J -0.1694 Soluble
172 G J -0.1872 Soluble
173 V J 0.1762 Aggregation-prone
174 H J -0.1084 Soluble
175 T J 0.0446 Aggregation-prone
176 F J 0.0000 Buried
177 P J -0.2202 Soluble
178 A J 0.3526 Aggregation-prone
179 V J 0.6257 Aggregation-prone
180 L J 1.4055 Aggregation-prone
181 Q J 0.3668 Aggregation-prone
182 S J -0.0138 Soluble
183 S J -0.1788 Soluble
184 G J 0.0654 Aggregation-prone
185 L J 0.2316 Aggregation-prone
186 Y J 0.5459 Aggregation-prone
187 S J 0.0000 Buried
188 L J 0.0000 Buried
189 S J 0.0000 Buried
190 S J 0.0000 Buried
191 V J 0.0000 Buried
192 V J 0.0000 Buried
193 T J 0.0161 Aggregation-prone
194 V J 0.0288 Aggregation-prone
195 P J -0.2454 Soluble
196 Q J -1.4017 Soluble
197 T J -0.9781 Soluble
198 Y J -0.7915 Soluble
199 T J -0.9271 Soluble
200 C J 0.0000 Buried
201 N J -1.3394 Soluble
202 V J 0.0000 Buried
203 D J -1.7509 Soluble
204 H J 0.0000 Buried
205 K J -2.7009 Soluble
206 P J -1.5757 Soluble
207 S J -1.8068 Soluble
208 N J -2.5465 Soluble
209 T J -2.0210 Soluble
210 K J -2.6332 Soluble
211 V J -1.4641 Soluble
212 D J -2.2660 Soluble
213 K J -1.5518 Soluble
214 T J -1.0815 Soluble
215 V J 0.0000 Buried
216 E J -1.9863 Soluble
1 D K -2.2346 Soluble
2 I K 0.0000 Buried
3 Q K -2.0835 Soluble
4 M K 0.0000 Buried
5 T K -1.0189 Soluble
6 Q K 0.0000 Buried
7 S K -0.4872 Soluble
8 P K -0.6182 Soluble
9 S K -0.8842 Soluble
10 S K -0.9893 Soluble
11 L K -0.5989 Soluble
12 S K -0.7184 Soluble
13 A K -1.0160 Soluble
14 S K -1.0154 Soluble
15 V K -0.2603 Soluble
16 G K -0.8818 Soluble
17 D K -1.9558 Soluble
18 R K -2.4409 Soluble
19 V K 0.0000 Buried
20 T K -0.6653 Soluble
21 I K 0.0000 Buried
22 T K -0.7253 Soluble
23 C K 0.0000 Buried
24 Q K -2.0400 Soluble
25 A K -2.0342 Soluble
26 S K -2.1619 Soluble
27 Q K -2.9828 Soluble
28 D K -2.9100 Soluble
29 I K 0.0000 Buried
36 S K -1.3125 Soluble
37 N K -1.0760 Soluble
38 Y K -0.2559 Soluble
39 L K 0.0000 Buried
40 N K 0.0000 Buried
41 W K 0.0000 Buried
42 Y K 0.0000 Buried
43 Q K 0.0000 Buried
44 Q K -1.5391 Soluble
45 K K -2.0607 Soluble
46 P K -1.6315 Soluble
47 G K -1.8822 Soluble
48 K K -2.6266 Soluble
49 A K -1.6830 Soluble
50 P K 0.0000 Buried
51 K K -1.7002 Soluble
52 L K 0.0000 Buried
53 L K 0.0000 Buried
54 I K 0.0000 Buried
55 Y K -0.4836 Soluble
56 D K -0.9926 Soluble
57 A K 0.0000 Buried
65 S K -1.0345 Soluble
66 N K -1.0722 Soluble
67 L K -0.3900 Soluble
68 E K -0.6996 Soluble
69 T K -0.4581 Soluble
70 G K -0.5538 Soluble
71 V K 0.0000 Buried
72 P K -0.4208 Soluble
74 S K -0.4757 Soluble
75 R K -0.7242 Soluble
76 F K 0.0000 Buried
77 S K -0.4947 Soluble
78 G K -0.5546 Soluble
79 S K -1.0187 Soluble
80 G K -1.2368 Soluble
83 S K -1.3606 Soluble
84 G K -1.8509 Soluble
85 T K -2.3076 Soluble
86 D K -2.6753 Soluble
87 F K 0.0000 Buried
88 T K -0.7519 Soluble
89 F K 0.0000 Buried
90 T K -0.6093 Soluble
91 I K 0.0000 Buried
92 S K -1.3335 Soluble
93 S K -1.1715 Soluble
94 L K 0.0000 Buried
95 Q K -0.8011 Soluble
96 P K -1.4280 Soluble
97 E K -1.7104 Soluble
98 D K 0.0000 Buried
99 I K 0.0000 Buried
100 A K 0.0000 Buried
101 T K -1.1053 Soluble
102 Y K 0.0000 Buried
103 F K 0.0000 Buried
104 C K 0.0000 Buried
105 Q K 0.0000 Buried
106 H K 0.0000 Buried
107 F K -0.0448 Soluble
108 D K -0.9729 Soluble
109 H K -0.7734 Soluble
114 L K -0.0364 Soluble
115 P K 0.0000 Buried
116 L K 0.0000 Buried
117 A K -0.6054 Soluble
118 F K 0.0000 Buried
119 G K 0.0000 Buried
120 G K -1.1294 Soluble
121 G K -0.8663 Soluble
122 T K 0.0000 Buried
123 K K -1.9838 Soluble
124 V K 0.0000 Buried
125 E K -1.3063 Soluble
126 I K 0.0000 Buried
127 K K -1.3779 Soluble
128 R K -0.9884 Soluble
129 T K -0.1028 Soluble
130 V K 0.5188 Aggregation-prone
131 A K 0.1222 Aggregation-prone
132 A K -0.0544 Soluble
133 P K 0.0000 Buried
134 S K -0.1255 Soluble
135 V K 0.0000 Buried
136 F K 0.0676 Aggregation-prone
137 I K 0.0316 Aggregation-prone
138 F K 0.0000 Buried
139 P K -0.4190 Soluble
140 P K -0.8021 Soluble
141 S K -1.7219 Soluble
142 D K -3.0856 Soluble
143 E K -3.2454 Soluble
144 Q K 0.0000 Buried
145 L K -2.4574 Soluble
146 K K -2.9196 Soluble
147 S K -1.8305 Soluble
148 G K -1.4942 Soluble
149 T K -1.1693 Soluble
150 A K 0.0000 Buried
151 S K 0.0000 Buried
152 V K 0.0000 Buried
153 V K 0.0000 Buried
154 C K 0.0000 Buried
155 L K 0.0000 Buried
156 L K 0.0000 Buried
157 N K 0.0000 Buried
158 N K -0.7432 Soluble
159 F K 0.0000 Buried
160 Y K -0.8055 Soluble
161 P K -1.3056 Soluble
162 R K -2.1396 Soluble
163 E K -2.8282 Soluble
164 A K -2.1093 Soluble
165 K K -2.2917 Soluble
166 V K -0.9348 Soluble
167 Q K -0.4719 Soluble
168 W K 0.0000 Buried
169 K K -0.4504 Soluble
170 V K 0.0000 Buried
171 D K -1.6434 Soluble
172 N K -1.2433 Soluble
173 A K -0.2046 Soluble
174 L K 0.7695 Aggregation-prone
175 Q K -0.1470 Soluble
176 S K -0.4419 Soluble
177 G K -0.9034 Soluble
178 N K -0.8002 Soluble
179 S K -1.0734 Soluble
180 Q K -1.3406 Soluble
181 E K -1.4727 Soluble
182 S K -0.6415 Soluble
183 V K -0.2020 Soluble
184 T K -0.7829 Soluble
185 E K -1.8882 Soluble
186 Q K 0.0000 Buried
187 D K -1.9722 Soluble
188 S K -2.1666 Soluble
189 K K -2.4796 Soluble
190 D K -1.7584 Soluble
191 S K 0.0000 Buried
192 T K 0.0000 Buried
193 Y K 0.0000 Buried
194 S K 0.0000 Buried
195 L K 0.0000 Buried
196 S K 0.0000 Buried
197 S K 0.0000 Buried
198 T K -0.5781 Soluble
199 L K 0.0000 Buried
200 T K -0.3347 Soluble
201 L K -0.8065 Soluble
202 S K -1.3075 Soluble
203 K K -2.3598 Soluble
204 A K -2.2613 Soluble
205 D K -3.2660 Soluble
206 Y K 0.0000 Buried
207 E K -3.8636 Soluble
208 K K -3.8872 Soluble
209 H K -2.9866 Soluble
210 K K -2.6269 Soluble
211 V K -0.8873 Soluble
212 Y K 0.0000 Buried
213 A K 0.0000 Buried
214 C K 0.0000 Buried
215 E K -0.7297 Soluble
216 V K 0.0000 Buried
217 T K -1.1494 Soluble
218 H K 0.0000 Buried
219 Q K -1.6175 Soluble
220 G K -0.3733 Soluble
221 L K -0.2318 Soluble
222 S K -0.4526 Soluble
223 S K -0.3939 Soluble
224 P K -0.4768 Soluble
225 V K 0.0963 Aggregation-prone
226 T K -0.3850 Soluble
227 K K -0.6821 Soluble
228 S K -0.5321 Soluble
229 F K 0.0000 Buried
230 N K -1.7035 Soluble
231 R K -2.2310 Soluble
232 G K -2.0337 Soluble
233 E K -2.4619 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6704 1.8098 CSV PDB
4.5 -0.7162 1.764 CSV PDB
5.0 -0.7689 1.7435 CSV PDB
5.5 -0.8186 1.7355 CSV PDB
6.0 -0.8547 1.7355 CSV PDB
6.5 -0.8693 1.7355 CSV PDB
7.0 -0.8628 1.7355 CSV PDB
7.5 -0.8422 1.7355 CSV PDB
8.0 -0.8132 1.7355 CSV PDB
8.5 -0.7772 1.7355 CSV PDB
9.0 -0.733 1.7355 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH