Atisnolerbart / 7n0v

A4DAB027_7n0v_H_L | Source PDB: 7n0v | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6593
Max Score
1.1538
Min Score
-3.6272
Total Score
-280.21
Score CDR
-0.2998
Score non-CDR
-0.7051

Calculation Parameters

Chain Sequence(s) QVQLVQSGAEVKKPGASVKVSCKASGYTFISYNIFWVRQATGQGLDWMGWMNPFRNNAGYAQKFQGRVTVTWDTSISTAYMELSSLSSEDTAIYYCAREHGSSWGFFDYWGQGTLVTVSSASTKGPSVFPLAPCSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSKTYTCNVDHKPSNTKVDKRVEEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6272
Maximum Score 1.1538
Average Score -0.6593
Total Score -280.207

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.4845 Soluble
2 V H -0.8043 Soluble
3 Q H -1.1200 Soluble
4 L H 0.0000 Buried
5 V H 0.2005 Aggregation-prone
6 Q H -0.3191 Soluble
7 S H -0.5595 Soluble
8 G H -0.4841 Soluble
9 A H 0.2906 Aggregation-prone
11 E H 0.1409 Aggregation-prone
12 V H 1.0196 Aggregation-prone
13 K H -0.7353 Soluble
14 K H -1.8789 Soluble
15 P H -1.4365 Soluble
16 G H -1.1885 Soluble
17 A H -0.7989 Soluble
18 S H -1.1905 Soluble
19 V H 0.0000 Buried
20 K H -1.9546 Soluble
21 V H 0.0000 Buried
22 S H -0.6009 Soluble
23 C H 0.0000 Buried
24 K H -0.5884 Soluble
25 A H 0.0000 Buried
26 S H -0.5786 Soluble
27 G H -1.0002 Soluble
28 Y H -0.2553 Soluble
29 T H 0.1735 Aggregation-prone
30 F H 0.0000 Buried
35 I H 1.0459 Aggregation-prone
36 S H 0.5700 Aggregation-prone
37 Y H 0.6007 Aggregation-prone
38 N H 0.1617 Aggregation-prone
39 I H 0.0000 Buried
40 F H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H -0.3746 Soluble
44 Q H -0.4279 Soluble
45 A H -0.7530 Soluble
46 T H -0.6674 Soluble
47 G H -1.3267 Soluble
48 Q H -1.8151 Soluble
49 G H -1.2462 Soluble
50 L H 0.0000 Buried
51 D H -0.7958 Soluble
52 W H -0.2033 Soluble
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 W H 0.2724 Aggregation-prone
56 M H 0.0000 Buried
57 N H 0.0000 Buried
58 P H 0.0000 Buried
59 F H 0.7036 Aggregation-prone
62 R H -1.8235 Soluble
63 N H -2.0621 Soluble
64 N H -2.1058 Soluble
65 A H -0.9373 Soluble
66 G H -0.0265 Soluble
67 Y H -0.1448 Soluble
68 A H 0.0000 Buried
69 Q H -2.3814 Soluble
70 K H -2.7089 Soluble
71 F H 0.0000 Buried
72 Q H -2.3969 Soluble
74 G H -1.6167 Soluble
75 R H -1.5102 Soluble
76 V H 0.0000 Buried
77 T H -0.5738 Soluble
78 V H 0.0000 Buried
79 T H -0.5733 Soluble
80 W H -0.1890 Soluble
81 D H -0.3025 Soluble
82 T H 0.3342 Aggregation-prone
83 S H 0.3750 Aggregation-prone
84 I H 1.1538 Aggregation-prone
85 S H 0.3357 Aggregation-prone
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.6100 Soluble
89 M H 0.0000 Buried
90 E H -1.5918 Soluble
91 L H 0.0000 Buried
92 S H -0.9964 Soluble
93 S H -0.8462 Soluble
94 L H 0.0000 Buried
95 S H -1.3815 Soluble
96 S H -1.5389 Soluble
97 E H -2.0163 Soluble
98 D H 0.0000 Buried
99 T H -0.3691 Soluble
100 A H 0.0000 Buried
101 I H 0.5640 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 E H 0.0000 Buried
108 H H -0.4544 Soluble
109 G H -0.3339 Soluble
110 S H -0.2938 Soluble
111 S H -0.1757 Soluble
112 W H 0.2364 Aggregation-prone
113 G H 0.0000 Buried
114 F H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.3928 Soluble
117 Y H -0.1748 Soluble
118 W H -0.4138 Soluble
119 G H 0.0000 Buried
120 Q H -1.2894 Soluble
121 G H -0.5669 Soluble
122 T H 0.0000 Buried
123 L H 0.9047 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.8309 Soluble
128 S H -0.7852 Soluble
129 A H -0.6813 Soluble
130 S H -0.7128 Soluble
131 T H -0.9070 Soluble
132 K H -1.3127 Soluble
133 G H -1.4029 Soluble
134 P H 0.0000 Buried
135 S H -0.4181 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2226 Soluble
139 L H 0.0000 Buried
140 A H -0.3548 Soluble
141 P H 0.1221 Aggregation-prone
142 C H 0.4753 Aggregation-prone
143 S H -0.4274 Soluble
144 T H -0.4023 Soluble
145 A H -0.2549 Soluble
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.5388 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H -0.6992 Soluble
157 E H -1.2146 Soluble
158 P H -1.1733 Soluble
159 V H 0.0000 Buried
160 T H -0.6747 Soluble
161 V H -0.1871 Soluble
162 S H -0.4523 Soluble
163 W H 0.0000 Buried
164 N H -1.0209 Soluble
165 S H -0.7836 Soluble
166 G H -0.5737 Soluble
167 A H -0.2505 Soluble
168 L H -0.0350 Soluble
169 T H -0.1486 Soluble
170 S H -0.1466 Soluble
171 G H -0.1431 Soluble
172 V H 0.2043 Aggregation-prone
173 H H -0.0962 Soluble
174 T H -0.0020 Soluble
175 F H 0.0000 Buried
176 P H -0.3997 Soluble
177 A H -0.0020 Soluble
178 V H 0.2566 Aggregation-prone
179 L H 0.8895 Aggregation-prone
180 Q H 0.0607 Aggregation-prone
181 S H -0.1068 Soluble
182 S H -0.2480 Soluble
183 G H -0.0129 Soluble
184 L H -0.0912 Soluble
185 Y H 0.0000 Buried
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1011 Soluble
193 V H 0.0000 Buried
194 P H -0.5532 Soluble
195 S H -0.5835 Soluble
196 S H -0.7261 Soluble
197 S H -0.9460 Soluble
198 K H -2.0258 Soluble
199 T H -1.6741 Soluble
200 Y H 0.0000 Buried
201 T H 0.0000 Buried
202 C H 0.0000 Buried
203 N H -1.5503 Soluble
204 V H 0.0000 Buried
205 D H -1.8630 Soluble
206 H H 0.0000 Buried
207 K H -2.8980 Soluble
208 P H -1.8392 Soluble
209 S H -1.9228 Soluble
210 N H -2.5903 Soluble
211 T H -2.0813 Soluble
212 K H -2.6523 Soluble
213 V H -1.6650 Soluble
214 D H -2.6102 Soluble
215 K H -2.1119 Soluble
216 R H -2.7980 Soluble
217 V H 0.0000 Buried
218 E H -2.4763 Soluble
1 E L -1.5710 Soluble
2 I L 0.0000 Buried
3 V L 0.5826 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.7402 Soluble
6 Q L 0.0000 Buried
7 S L -0.9010 Soluble
8 P L -0.5259 Soluble
9 G L -0.8642 Soluble
10 T L -0.4602 Soluble
11 L L -0.1617 Soluble
12 S L -0.3086 Soluble
13 L L -0.7615 Soluble
14 S L -1.4337 Soluble
15 P L -1.8683 Soluble
16 G L -2.1996 Soluble
17 E L -2.9105 Soluble
18 R L -3.1468 Soluble
19 A L 0.0000 Buried
20 T L -0.6697 Soluble
21 L L 0.0000 Buried
22 S L -0.9088 Soluble
23 C L 0.0000 Buried
24 R L -2.2602 Soluble
25 A L 0.0000 Buried
26 S L -1.0916 Soluble
27 Q L -1.7845 Soluble
28 S L -1.3868 Soluble
29 V L 0.0000 Buried
30 S L -0.5831 Soluble
36 S L -0.3777 Soluble
37 S L -0.4551 Soluble
38 Y L 0.0335 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.6899 Soluble
46 P L -1.3403 Soluble
47 G L -1.4280 Soluble
48 Q L -2.2246 Soluble
49 A L -1.5273 Soluble
50 P L 0.0000 Buried
51 R L -1.9751 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.1023 Soluble
56 G L -0.1747 Soluble
57 A L 0.0000 Buried
65 S L -0.6127 Soluble
66 S L -0.7000 Soluble
67 R L -1.4901 Soluble
68 A L -0.8726 Soluble
69 T L -0.5523 Soluble
70 G L -0.7904 Soluble
71 I L -1.0467 Soluble
72 P L -1.2797 Soluble
74 D L -2.2199 Soluble
75 R L -2.0477 Soluble
76 F L 0.0000 Buried
77 S L -0.9076 Soluble
78 G L 0.0000 Buried
79 S L -0.6295 Soluble
80 G L -1.0662 Soluble
83 S L -0.8600 Soluble
84 G L -1.1800 Soluble
85 T L -1.6688 Soluble
86 D L -2.0626 Soluble
87 F L 0.0000 Buried
88 T L -0.7632 Soluble
89 L L 0.0000 Buried
90 T L -0.8320 Soluble
91 I L 0.0000 Buried
92 S L -2.4979 Soluble
93 R L -3.3223 Soluble
94 L L 0.0000 Buried
95 E L -2.3204 Soluble
96 P L -1.5236 Soluble
97 E L -2.2883 Soluble
98 D L 0.0000 Buried
99 F L -0.6284 Soluble
100 A L 0.0000 Buried
101 V L -0.3199 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 G L -0.3612 Soluble
109 S L -0.7584 Soluble
114 S L -0.6396 Soluble
115 P L -0.6966 Soluble
116 W L -0.0879 Soluble
117 T L 0.0122 Aggregation-prone
118 F L 0.2695 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.2771 Soluble
121 G L -0.9336 Soluble
122 T L 0.0000 Buried
123 K L -0.7653 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.5493 Soluble
127 K L -1.2487 Soluble
128 R L -0.8269 Soluble
129 T L 0.1895 Aggregation-prone
130 V L 0.9502 Aggregation-prone
131 A L 0.4855 Aggregation-prone
132 A L 0.0798 Aggregation-prone
133 P L 0.0000 Buried
134 S L -0.1468 Soluble
135 V L 0.0000 Buried
136 F L 0.0728 Aggregation-prone
137 I L 0.1093 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.0949 Soluble
140 P L 0.0000 Buried
141 S L -1.5616 Soluble
142 D L -3.0023 Soluble
143 E L -3.1218 Soluble
144 Q L 0.0000 Buried
145 L L -2.3230 Soluble
146 K L -2.9133 Soluble
147 S L -1.7905 Soluble
148 G L -1.2655 Soluble
149 T L -1.0332 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.6911 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.5736 Soluble
162 R L -2.8208 Soluble
163 E L -3.1325 Soluble
164 A L -2.2453 Soluble
165 K L -2.2622 Soluble
166 V L -1.0387 Soluble
167 Q L -0.7242 Soluble
168 W L 0.0000 Buried
169 K L -0.8286 Soluble
170 V L 0.0000 Buried
171 D L -1.7993 Soluble
172 N L -1.5383 Soluble
173 A L -0.5612 Soluble
174 L L 0.1822 Aggregation-prone
175 Q L -0.5434 Soluble
176 S L -0.8444 Soluble
177 G L -1.3235 Soluble
178 N L -1.6188 Soluble
179 S L -1.6618 Soluble
180 Q L -1.9162 Soluble
181 E L -1.8006 Soluble
182 S L -0.7765 Soluble
183 V L -0.7551 Soluble
184 T L -1.1990 Soluble
185 E L -2.3221 Soluble
186 Q L -2.1136 Soluble
187 D L -2.1785 Soluble
188 S L -1.8752 Soluble
189 K L -2.3115 Soluble
190 D L -1.7951 Soluble
191 S L -1.8016 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8056 Soluble
199 L L 0.0000 Buried
200 T L -0.6947 Soluble
201 L L -0.8079 Soluble
202 S L -1.0751 Soluble
203 K L -2.1117 Soluble
204 A L -1.9139 Soluble
205 D L -2.5477 Soluble
206 Y L 0.0000 Buried
207 E L -3.6272 Soluble
208 K L -3.5573 Soluble
209 H L -2.9318 Soluble
210 K L -2.8550 Soluble
211 V L -1.1750 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8219 Soluble
216 V L 0.0000 Buried
217 T L -1.1435 Soluble
218 H L 0.0000 Buried
219 Q L -1.6167 Soluble
220 G L -0.2171 Soluble
221 L L -0.0900 Soluble
222 S L -0.4143 Soluble
223 S L -0.3938 Soluble
224 P L -0.5447 Soluble
225 V L -0.0118 Soluble
226 T L -0.4550 Soluble
227 K L -0.6895 Soluble
228 S L -0.4125 Soluble
229 F L 0.0000 Buried
230 N L -1.4648 Soluble
231 R L -2.1176 Soluble
232 G L -1.9895 Soluble
233 E L -1.9925 Soluble
234 C L -0.2532 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6936 2.5849 CSV PDB
4.5 -0.7348 2.5254 CSV PDB
5.0 -0.7831 2.4485 CSV PDB
5.5 -0.8284 2.367 CSV PDB
6.0 -0.8602 2.2934 CSV PDB
6.5 -0.8705 2.2401 CSV PDB
7.0 -0.859 2.2116 CSV PDB
7.5 -0.833 2.2 CSV PDB
8.0 -0.7992 2.1959 CSV PDB
8.5 -0.7599 2.1946 CSV PDB
9.0 -0.7153 2.1942 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH