Coltuximab / 6ani

A4DAB069_6ani_H_L | Source PDB: 6ani | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7036
Max Score
1.7331
Min Score
-3.8858
Total Score
-299.73
Score CDR
-0.3948
Score non-CDR
-0.7391

Calculation Parameters

Chain Sequence(s) QVQLVQPGAEVVKPGASVKLSCKTSGYTFTSNWMHWVKQAPGQGLEWIGEIDPSDSYTNYNQNFQGKAKLTVDKSTSTAYMEVSSLRSDDTAVYYCARGSNPYYYAMDYWGQGTSVTVSSASTKGPSVFPLAPSSKGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPEIVLTQSPAIMSASPGERVTMTCSASSGVNYMHWYQQKPGTSPRRWIYDTSKLASGVPARFSGSGSGTDYSLTISSMEPEDAATYYCHQRGSYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8858
Maximum Score 1.7331
Average Score -0.7036
Total Score -299.7255

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.4549 Soluble
2 V H -0.9917 Soluble
3 Q H -1.7108 Soluble
4 L H 0.0000 Buried
5 V H -0.8909 Soluble
6 Q H 0.0000 Buried
7 P H -0.8286 Soluble
8 G H -0.8320 Soluble
9 A H -0.4017 Soluble
11 E H -0.3872 Soluble
12 V H 0.2194 Aggregation-prone
13 V H -0.6837 Soluble
14 K H -1.9967 Soluble
15 P H -1.8447 Soluble
16 G H -1.5587 Soluble
17 A H -1.1088 Soluble
18 S H -1.2961 Soluble
19 V H 0.0000 Buried
20 K H -2.0464 Soluble
21 L H 0.0000 Buried
22 S H -0.8102 Soluble
23 C H 0.0000 Buried
24 K H -1.6612 Soluble
25 T H 0.0000 Buried
26 S H -1.1840 Soluble
27 G H -1.0238 Soluble
28 Y H -0.4393 Soluble
29 T H -0.3021 Soluble
30 F H 0.0000 Buried
35 T H -0.6961 Soluble
36 S H -0.4210 Soluble
37 N H 0.0000 Buried
38 W H -0.0473 Soluble
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 K H -0.4916 Soluble
44 Q H -0.7349 Soluble
45 A H -1.0650 Soluble
46 P H -0.9013 Soluble
47 G H -1.2381 Soluble
48 Q H -1.7843 Soluble
49 G H -1.2415 Soluble
50 L H 0.0000 Buried
51 E H -0.7040 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 E H 0.0000 Buried
56 I H 0.0000 Buried
57 D H -0.3704 Soluble
58 P H 0.0000 Buried
59 S H -1.1293 Soluble
62 D H -1.5778 Soluble
63 S H -0.7361 Soluble
64 Y H 0.4403 Aggregation-prone
65 T H -0.0478 Soluble
66 N H -0.2052 Soluble
67 Y H -0.9382 Soluble
68 N H 0.0000 Buried
69 Q H -2.1769 Soluble
70 N H -2.1529 Soluble
71 F H -1.8344 Soluble
72 Q H -2.4033 Soluble
74 G H -1.9368 Soluble
75 K H -1.7877 Soluble
76 A H 0.0000 Buried
77 K H -2.3856 Soluble
78 L H 0.0000 Buried
79 T H -0.6826 Soluble
80 V H -0.8924 Soluble
81 D H -1.4322 Soluble
82 K H -1.8167 Soluble
83 S H -1.0911 Soluble
84 T H -1.0853 Soluble
85 S H -1.2733 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.8667 Soluble
89 M H 0.0000 Buried
90 E H -2.1715 Soluble
91 V H 0.0000 Buried
92 S H -1.4261 Soluble
93 S H -1.2539 Soluble
94 L H 0.0000 Buried
95 R H -3.0234 Soluble
96 S H -2.2565 Soluble
97 D H -2.4986 Soluble
98 D H 0.0000 Buried
99 T H -0.9155 Soluble
100 A H 0.0000 Buried
101 V H -0.0611 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.2449 Soluble
107 G H 0.0000 Buried
108 S H 0.0000 Buried
109 N H -1.0527 Soluble
110 P H -0.3834 Soluble
111 Y H 0.0504 Aggregation-prone
112 Y H 0.5414 Aggregation-prone
113 Y H 0.3594 Aggregation-prone
114 A H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.4063 Soluble
117 Y H -0.3821 Soluble
118 W H -0.5209 Soluble
119 G H 0.0000 Buried
120 Q H -1.3211 Soluble
121 G H 0.0000 Buried
122 T H 0.0000 Buried
123 S H -0.2883 Soluble
124 V H 0.0000 Buried
125 T H -0.5796 Soluble
126 V H 0.0000 Buried
127 S H -0.8045 Soluble
128 S H -0.7264 Soluble
129 A H -0.4789 Soluble
130 S H -0.5103 Soluble
131 T H -0.7309 Soluble
132 K H -1.1894 Soluble
133 G H -1.3567 Soluble
134 P H 0.0000 Buried
135 S H -0.4046 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.0627 Soluble
139 L H 0.0000 Buried
140 A H -0.7377 Soluble
141 P H 0.0000 Buried
142 S H -0.9713 Soluble
143 S H -1.1922 Soluble
144 K H -1.9446 Soluble
145 G H -0.8543 Soluble
146 T H -0.6560 Soluble
147 A H -0.5435 Soluble
148 A H 0.0000 Buried
149 L H 0.0000 Buried
150 G H 0.0000 Buried
151 C H 0.0000 Buried
152 L H 0.0000 Buried
153 V H 0.0000 Buried
154 K H 0.0000 Buried
155 D H -0.3861 Soluble
156 Y H 0.0000 Buried
157 F H 0.0000 Buried
158 P H -0.8000 Soluble
159 E H -1.0019 Soluble
160 P H -1.0769 Soluble
161 V H -0.8033 Soluble
162 T H -0.6137 Soluble
163 V H -0.1184 Soluble
164 S H -0.3838 Soluble
165 W H 0.0000 Buried
166 N H -0.6697 Soluble
167 S H -0.6417 Soluble
168 G H -0.5067 Soluble
169 A H -0.2376 Soluble
170 L H 0.0251 Aggregation-prone
171 T H -0.1692 Soluble
172 S H -0.1917 Soluble
173 G H -0.2177 Soluble
174 V H 0.1808 Aggregation-prone
175 H H -0.1282 Soluble
176 T H 0.0150 Aggregation-prone
177 F H 0.0000 Buried
178 P H -0.4342 Soluble
179 A H 0.0025 Aggregation-prone
180 V H 0.2932 Aggregation-prone
181 L H 0.7381 Aggregation-prone
182 Q H 0.0991 Aggregation-prone
183 S H -0.1323 Soluble
184 S H -0.2440 Soluble
185 G H -0.0519 Soluble
186 L H -0.0427 Soluble
187 Y H 0.2458 Aggregation-prone
188 S H 0.0000 Buried
189 L H 0.0000 Buried
190 S H 0.0000 Buried
191 S H 0.0000 Buried
192 V H 0.0000 Buried
193 V H 0.0000 Buried
194 T H -0.1343 Soluble
195 V H 0.0000 Buried
196 P H -0.5577 Soluble
197 S H -0.7076 Soluble
198 S H -0.5842 Soluble
199 S H -0.5884 Soluble
200 L H -0.7127 Soluble
201 G H -0.9140 Soluble
202 T H -0.7183 Soluble
203 Q H -1.1805 Soluble
204 T H -0.9782 Soluble
205 Y H 0.0000 Buried
206 I H -1.0363 Soluble
207 C H 0.0000 Buried
208 N H -1.4008 Soluble
209 V H 0.0000 Buried
210 N H -1.9614 Soluble
211 H H 0.0000 Buried
212 K H -2.8051 Soluble
213 P H -1.6921 Soluble
214 S H -1.8449 Soluble
215 N H -2.5434 Soluble
216 T H -2.0624 Soluble
217 K H -2.6911 Soluble
218 V H -1.5801 Soluble
219 D H -2.4169 Soluble
220 K H -1.7681 Soluble
221 K H -2.0193 Soluble
222 V H 0.0000 Buried
223 E H -2.0548 Soluble
224 P H -1.0900 Soluble
1 E L -1.3184 Soluble
2 I L 0.0866 Aggregation-prone
3 V L 1.3451 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.1384 Soluble
6 Q L -0.4137 Soluble
7 S L -0.1189 Soluble
8 P L 0.2564 Aggregation-prone
9 A L 0.6580 Aggregation-prone
10 I L 1.7331 Aggregation-prone
11 M L 0.6773 Aggregation-prone
12 S L -0.3832 Soluble
13 A L 0.0000 Buried
14 S L -1.9144 Soluble
15 P L -1.9029 Soluble
16 G L -2.0401 Soluble
17 E L -2.9979 Soluble
18 R L -2.7947 Soluble
19 V L 0.0000 Buried
20 T L -0.6932 Soluble
21 M L 0.0000 Buried
22 T L -0.6528 Soluble
23 C L 0.0000 Buried
24 S L -0.7709 Soluble
25 A L 0.0000 Buried
26 S L -0.1540 Soluble
27 S L -0.5086 Soluble
28 G L -1.1231 Soluble
29 V L -1.1313 Soluble
37 N L -1.3137 Soluble
38 Y L -0.8522 Soluble
39 M L 0.0000 Buried
40 H L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.3548 Soluble
46 P L -1.1573 Soluble
47 G L -0.9176 Soluble
48 T L -1.0290 Soluble
49 S L -0.9658 Soluble
50 P L 0.0000 Buried
51 R L -1.6521 Soluble
52 R L -0.8147 Soluble
53 W L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.0705 Soluble
56 D L -0.3411 Soluble
57 T L -0.6217 Soluble
65 S L -0.8090 Soluble
66 K L -0.9476 Soluble
67 L L -0.2125 Soluble
68 A L 0.0000 Buried
69 S L -0.4543 Soluble
70 G L -0.4156 Soluble
71 V L -0.1975 Soluble
72 P L -0.1352 Soluble
74 A L -0.0874 Soluble
75 R L -0.6560 Soluble
76 F L 0.0000 Buried
77 S L -0.4565 Soluble
78 G L -0.5616 Soluble
79 S L -0.9416 Soluble
80 G L -1.3066 Soluble
83 S L -1.3572 Soluble
84 G L -1.4088 Soluble
85 T L -1.5308 Soluble
86 D L -2.1871 Soluble
87 Y L 0.0000 Buried
88 S L -0.7458 Soluble
89 L L 0.0000 Buried
90 T L -0.6097 Soluble
91 I L 0.0000 Buried
92 S L -1.4032 Soluble
93 S L -1.8255 Soluble
94 M L 0.0000 Buried
95 E L -2.0844 Soluble
96 P L -1.8627 Soluble
97 E L -2.3022 Soluble
98 D L 0.0000 Buried
99 A L -1.1176 Soluble
100 A L 0.0000 Buried
101 T L -0.2365 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 H L 0.0000 Buried
106 Q L -0.5138 Soluble
107 R L -0.9980 Soluble
108 G L -0.6551 Soluble
115 S L 0.0000 Buried
116 Y L -0.4387 Soluble
117 T L -0.1957 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -0.6944 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L 0.0983 Aggregation-prone
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -1.3177 Soluble
127 K L -1.7993 Soluble
128 R L -1.0807 Soluble
129 T L -0.0845 Soluble
130 V L 0.7007 Aggregation-prone
131 A L 0.2381 Aggregation-prone
132 A L -0.0107 Soluble
133 P L 0.0000 Buried
134 S L -0.1151 Soluble
135 V L 0.0000 Buried
136 F L -0.0444 Soluble
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -0.8236 Soluble
140 P L 0.0000 Buried
141 S L -1.6946 Soluble
142 D L -3.0895 Soluble
143 E L -3.2640 Soluble
144 Q L 0.0000 Buried
145 L L -2.4464 Soluble
146 K L -2.9582 Soluble
147 S L -1.8387 Soluble
148 G L -1.4615 Soluble
149 T L -1.1103 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.6713 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.2648 Soluble
162 R L -1.9136 Soluble
163 E L -2.7350 Soluble
164 A L -1.9262 Soluble
165 K L -1.8124 Soluble
166 V L -0.7757 Soluble
167 Q L -0.3468 Soluble
168 W L 0.0000 Buried
169 K L -0.5843 Soluble
170 V L 0.0000 Buried
171 D L -1.8097 Soluble
172 N L -1.3860 Soluble
173 A L -0.3384 Soluble
174 L L 0.7535 Aggregation-prone
175 Q L -0.3925 Soluble
176 S L -0.6645 Soluble
177 G L -1.3653 Soluble
178 N L -1.7493 Soluble
179 S L -1.6359 Soluble
180 Q L -1.6822 Soluble
181 E L -1.9724 Soluble
182 S L -0.8818 Soluble
183 V L -0.7662 Soluble
184 T L -1.0164 Soluble
185 E L -2.0809 Soluble
186 Q L -1.7727 Soluble
187 D L -1.8991 Soluble
188 S L -1.9392 Soluble
189 K L -2.2554 Soluble
190 D L -1.7339 Soluble
191 S L -1.7536 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8705 Soluble
199 L L 0.0000 Buried
200 T L -0.7001 Soluble
201 L L -0.9524 Soluble
202 S L -1.2249 Soluble
203 K L -2.3666 Soluble
204 A L -2.0883 Soluble
205 D L -2.7656 Soluble
206 Y L 0.0000 Buried
207 E L -3.8858 Soluble
208 K L -3.7535 Soluble
209 H L -3.1878 Soluble
210 K L -3.3917 Soluble
211 V L -1.8558 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7841 Soluble
216 V L 0.0000 Buried
217 T L -1.0308 Soluble
218 H L 0.0000 Buried
219 Q L -1.4871 Soluble
220 G L -0.4034 Soluble
221 L L -0.1901 Soluble
222 S L -0.4443 Soluble
223 S L -0.4172 Soluble
224 P L -0.4970 Soluble
225 V L -0.0728 Soluble
226 T L -0.5361 Soluble
227 K L -0.8036 Soluble
228 S L -0.7273 Soluble
229 F L -1.2679 Soluble
230 N L -2.3791 Soluble
231 R L -3.1200 Soluble
232 G L -2.3984 Soluble
233 E L -2.6751 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6735 2.7842 CSV PDB
4.5 -0.7158 2.7849 CSV PDB
5.0 -0.7655 2.7871 CSV PDB
5.5 -0.8131 2.7935 CSV PDB
6.0 -0.8478 2.8098 CSV PDB
6.5 -0.8602 2.8423 CSV PDB
7.0 -0.8487 2.8903 CSV PDB
7.5 -0.8199 2.947 CSV PDB
8.0 -0.781 3.0073 CSV PDB
8.5 -0.7348 3.0688 CSV PDB
9.0 -0.6822 3.1302 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH