Anzurstobart / 7st5

A4DAB022_7st5_H_L | Source PDB: 7st5 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6865
Max Score
1.2334
Min Score
-3.6578
Total Score
-286.27
Score CDR
-0.2983
Score non-CDR
-0.7358

Calculation Parameters

Chain Sequence(s) VQLVQSGAEVKKPGASVKVSCKASGYTFRGYGISWVRQAPGQGLEWMGWISAYGGETNYAQKLQGRVTMTTDTSTSTAYMELRSLRSDDTAVYYCAREAGSSWYDFDLWGRGTLVTVSSASTKGPSVFPLAPTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSQTYICNVNHKPSNTKVDKKVEPDIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGASFPITFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6578
Maximum Score 1.2334
Average Score -0.6865
Total Score -286.2749

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V H 1.2334 Aggregation-prone
3 Q H -0.3091 Soluble
4 L H 0.0000 Buried
5 V H 0.7366 Aggregation-prone
6 Q H 0.0000 Buried
7 S H -0.4773 Soluble
8 G H -0.3169 Soluble
9 A H -0.0206 Soluble
11 E H -0.2186 Soluble
12 V H 0.3900 Aggregation-prone
13 K H -1.1090 Soluble
14 K H -2.2624 Soluble
15 P H -2.3514 Soluble
16 G H -1.7607 Soluble
17 A H -1.3246 Soluble
18 S H -1.5755 Soluble
19 V H 0.0000 Buried
20 K H -1.9418 Soluble
21 V H 0.0000 Buried
22 S H -0.4157 Soluble
23 C H 0.0000 Buried
24 K H -0.8999 Soluble
25 A H 0.0000 Buried
26 S H -0.1157 Soluble
27 G H 0.0992 Aggregation-prone
28 Y H 0.0843 Aggregation-prone
29 T H -0.2454 Soluble
30 F H 0.0000 Buried
35 R H -0.7340 Soluble
36 G H -0.4024 Soluble
37 Y H 0.2036 Aggregation-prone
38 G H 0.0000 Buried
39 I H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.5964 Soluble
45 A H -0.9638 Soluble
46 P H -0.9612 Soluble
47 G H -1.2686 Soluble
48 Q H -1.7389 Soluble
49 G H -1.1578 Soluble
50 L H 0.0000 Buried
51 E H -0.6420 Soluble
52 W H 0.0000 Buried
53 M H 0.0000 Buried
54 G H 0.0000 Buried
55 W H -0.2250 Soluble
56 I H 0.0000 Buried
57 S H -0.6037 Soluble
58 A H 0.0000 Buried
59 Y H 0.3096 Aggregation-prone
62 G H -0.7640 Soluble
63 G H -1.3188 Soluble
64 E H -2.0378 Soluble
65 T H -1.2198 Soluble
66 N H -0.9251 Soluble
67 Y H -0.8822 Soluble
68 A H 0.0000 Buried
69 Q H -2.6531 Soluble
70 K H -2.6498 Soluble
71 L H 0.0000 Buried
72 Q H -2.2251 Soluble
74 G H -1.6192 Soluble
75 R H -1.4281 Soluble
76 V H 0.0000 Buried
77 T H -0.7099 Soluble
78 M H 0.0000 Buried
79 T H -0.3969 Soluble
80 T H -0.5504 Soluble
81 D H -1.0933 Soluble
82 T H -0.6247 Soluble
83 S H -0.5228 Soluble
84 T H -0.6557 Soluble
85 S H -0.8939 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.3644 Soluble
89 M H 0.0000 Buried
90 E H -1.2103 Soluble
91 L H 0.0000 Buried
92 R H -1.6716 Soluble
93 S H -1.5821 Soluble
94 L H 0.0000 Buried
95 R H -3.1649 Soluble
96 S H -2.3660 Soluble
97 D H -2.5107 Soluble
98 D H 0.0000 Buried
99 T H -0.8739 Soluble
100 A H 0.0000 Buried
101 V H 0.1296 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.2597 Soluble
107 E H 0.0000 Buried
108 A H -0.0801 Soluble
109 G H -0.0411 Soluble
110 S H -0.1949 Soluble
111 S H -0.1659 Soluble
112 W H 0.1814 Aggregation-prone
113 Y H 0.2866 Aggregation-prone
114 D H 0.0000 Buried
115 F H 0.0000 Buried
116 D H -0.7241 Soluble
117 L H -0.4379 Soluble
118 W H -0.8065 Soluble
119 G H 0.0000 Buried
120 R H -1.9279 Soluble
121 G H 0.0000 Buried
122 T H 0.0000 Buried
123 L H 0.3063 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.3564 Soluble
128 S H -1.1873 Soluble
129 A H -0.6819 Soluble
130 S H -0.8028 Soluble
131 T H -0.8336 Soluble
132 K H -1.8387 Soluble
133 G H -1.6999 Soluble
134 P H 0.0000 Buried
135 S H -0.5536 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.9725 Soluble
139 L H 0.0000 Buried
140 A H -0.8892 Soluble
141 P H -0.6280 Soluble
142 T H -0.2586 Soluble
143 A H -0.2066 Soluble
144 A H 0.0000 Buried
145 L H 0.0000 Buried
146 G H 0.0000 Buried
147 C H 0.0000 Buried
148 L H 0.0000 Buried
149 V H 0.0000 Buried
150 K H 0.0000 Buried
151 D H -0.6152 Soluble
152 Y H 0.0000 Buried
153 F H 0.0000 Buried
154 P H 0.0000 Buried
155 E H -0.6364 Soluble
156 P H -0.9446 Soluble
157 V H -0.8196 Soluble
158 T H -0.6685 Soluble
159 V H -0.3759 Soluble
160 S H -0.4378 Soluble
161 W H 0.0000 Buried
162 N H -0.8136 Soluble
163 S H -0.6462 Soluble
164 G H -0.5507 Soluble
165 A H -0.2156 Soluble
166 L H 0.0718 Aggregation-prone
167 T H -0.1387 Soluble
168 S H -0.1685 Soluble
169 G H -0.4543 Soluble
170 V H 0.1952 Aggregation-prone
171 H H 0.0000 Buried
172 T H 0.0646 Aggregation-prone
173 F H 0.0000 Buried
174 P H -0.4307 Soluble
175 A H -0.0037 Soluble
176 V H 0.1707 Aggregation-prone
177 L H 0.6977 Aggregation-prone
178 Q H 0.0693 Aggregation-prone
179 S H -0.2641 Soluble
180 S H -0.2795 Soluble
181 G H -0.1059 Soluble
182 L H -0.1976 Soluble
183 Y H 0.0000 Buried
184 S H 0.0000 Buried
185 L H 0.0000 Buried
186 S H 0.0000 Buried
187 S H 0.0000 Buried
188 V H 0.0000 Buried
189 V H 0.0000 Buried
190 T H -0.1686 Soluble
191 V H 0.0000 Buried
192 P H -0.3843 Soluble
193 S H -0.3870 Soluble
194 Q H -1.1864 Soluble
195 T H -1.1646 Soluble
196 Y H -1.1961 Soluble
197 I H -1.3636 Soluble
198 C H 0.0000 Buried
199 N H -1.4882 Soluble
200 V H 0.0000 Buried
201 N H -2.0935 Soluble
202 H H 0.0000 Buried
203 K H -2.7726 Soluble
204 P H -1.6310 Soluble
205 S H -1.9899 Soluble
206 N H -2.6019 Soluble
207 T H -2.1799 Soluble
208 K H -2.7051 Soluble
209 V H -1.4725 Soluble
210 D H -2.5484 Soluble
211 K H -2.0682 Soluble
212 K H -2.6608 Soluble
213 V H 0.0000 Buried
214 E H -2.4471 Soluble
215 P H -1.3840 Soluble
1 D L -2.2601 Soluble
2 I L -1.6053 Soluble
3 Q L -2.2022 Soluble
4 M L 0.0000 Buried
5 T L -1.3584 Soluble
6 Q L -1.0282 Soluble
7 S L -0.7091 Soluble
8 P L -0.5821 Soluble
9 S L -0.7831 Soluble
10 S L -0.7364 Soluble
11 V L -0.4487 Soluble
12 S L -0.9075 Soluble
13 A L 0.0000 Buried
14 S L -0.7350 Soluble
15 V L 0.0747 Aggregation-prone
16 G L -0.9339 Soluble
17 D L -1.9914 Soluble
18 R L -2.4608 Soluble
19 V L 0.0000 Buried
20 T L -0.6324 Soluble
21 I L 0.0000 Buried
22 T L -0.8556 Soluble
23 C L 0.0000 Buried
24 R L -2.8247 Soluble
25 A L 0.0000 Buried
26 S L -2.0610 Soluble
27 Q L -2.2784 Soluble
28 G L -1.4344 Soluble
29 I L 0.0000 Buried
36 S L -0.5026 Soluble
37 S L -0.3196 Soluble
38 W L 0.2090 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.4091 Soluble
45 K L -1.8834 Soluble
46 P L -1.6474 Soluble
47 G L -1.8054 Soluble
48 K L -2.8076 Soluble
49 A L -1.9163 Soluble
50 P L 0.0000 Buried
51 K L -1.6966 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.0867 Soluble
56 A L -0.1196 Soluble
57 A L 0.0000 Buried
65 S L -0.6863 Soluble
66 N L -0.9803 Soluble
67 L L -0.3882 Soluble
68 Q L -0.5973 Soluble
69 S L -0.5668 Soluble
70 G L -0.5378 Soluble
71 V L -0.2436 Soluble
72 P L -0.3812 Soluble
74 S L -0.4370 Soluble
75 R L -0.8837 Soluble
76 F L 0.0000 Buried
77 S L -0.5017 Soluble
78 G L -0.4475 Soluble
79 S L -0.7360 Soluble
80 G L -0.9589 Soluble
83 S L -0.7925 Soluble
84 G L -1.2634 Soluble
85 T L -1.8511 Soluble
86 D L -2.1728 Soluble
87 F L 0.0000 Buried
88 T L -0.7374 Soluble
89 L L 0.0000 Buried
90 T L -0.6250 Soluble
91 I L 0.0000 Buried
92 S L -1.3899 Soluble
93 S L -1.2552 Soluble
94 L L 0.0000 Buried
95 Q L -0.9185 Soluble
96 P L -0.9292 Soluble
97 E L -1.9211 Soluble
98 D L 0.0000 Buried
99 F L -0.5520 Soluble
100 A L 0.0000 Buried
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 G L 0.0000 Buried
108 A L 0.1460 Aggregation-prone
109 S L -0.0172 Soluble
114 F L 0.0768 Aggregation-prone
115 P L -0.4207 Soluble
116 I L 0.0000 Buried
117 T L -0.6081 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -1.1061 Soluble
121 G L -0.9082 Soluble
122 T L 0.0000 Buried
123 K L -1.3160 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.4431 Soluble
127 K L -1.3888 Soluble
128 R L -0.8660 Soluble
129 T L -0.0744 Soluble
130 V L 0.5883 Aggregation-prone
131 A L 0.1893 Aggregation-prone
132 A L -0.0833 Soluble
133 P L 0.0000 Buried
134 S L -0.0986 Soluble
135 V L 0.0985 Aggregation-prone
136 F L 0.2352 Aggregation-prone
137 I L 0.1684 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6689 Soluble
140 P L -0.7379 Soluble
141 S L -1.6322 Soluble
142 D L -3.0211 Soluble
143 E L -3.0194 Soluble
144 Q L 0.0000 Buried
145 L L -2.3012 Soluble
146 K L -2.9409 Soluble
147 S L -1.8309 Soluble
148 G L -1.4762 Soluble
149 T L -0.9576 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8838 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3123 Soluble
162 R L -2.2120 Soluble
163 E L -2.7881 Soluble
164 A L -2.0851 Soluble
165 K L -2.2350 Soluble
166 V L -1.1607 Soluble
167 Q L -0.5265 Soluble
168 W L 0.0000 Buried
169 K L -0.4127 Soluble
170 V L 0.0000 Buried
171 D L -1.8952 Soluble
172 N L -1.5422 Soluble
173 A L -0.2549 Soluble
174 L L 0.7554 Aggregation-prone
175 Q L -0.3080 Soluble
176 S L -0.6577 Soluble
177 G L -1.2875 Soluble
178 N L -1.6234 Soluble
179 S L -1.5172 Soluble
180 Q L -1.5935 Soluble
181 E L -1.9366 Soluble
182 S L -0.7934 Soluble
183 V L -0.5811 Soluble
184 T L -1.0035 Soluble
185 E L -2.0948 Soluble
186 Q L -1.6373 Soluble
187 D L -1.7734 Soluble
188 S L -1.6431 Soluble
189 K L -2.1493 Soluble
190 D L -1.5110 Soluble
191 S L -1.5343 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7495 Soluble
199 L L 0.0000 Buried
200 T L -0.5166 Soluble
201 L L -0.7176 Soluble
202 S L -1.0873 Soluble
203 K L -1.8431 Soluble
204 A L -1.6064 Soluble
205 D L -2.2182 Soluble
206 Y L 0.0000 Buried
207 E L -3.5039 Soluble
208 K L -3.3856 Soluble
209 H L -3.2241 Soluble
210 K L -3.6578 Soluble
211 V L -2.0670 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.7250 Soluble
216 V L 0.0000 Buried
217 T L -1.2120 Soluble
218 H L 0.0000 Buried
219 Q L -1.4580 Soluble
220 G L -0.3801 Soluble
221 L L -0.1475 Soluble
222 S L -0.4013 Soluble
223 S L -0.3951 Soluble
224 P L -0.4915 Soluble
225 V L 0.0655 Aggregation-prone
226 T L -0.3894 Soluble
227 K L -0.6363 Soluble
228 S L -0.8512 Soluble
229 F L -1.2503 Soluble
230 N L -2.6519 Soluble
231 R L -3.1365 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7515 2.4312 CSV PDB
4.5 -0.7906 2.389 CSV PDB
5.0 -0.8354 2.3211 CSV PDB
5.5 -0.8769 2.2377 CSV PDB
6.0 -0.9046 2.1524 CSV PDB
6.5 -0.9095 2.0784 CSV PDB
7.0 -0.8912 2.0281 CSV PDB
7.5 -0.8574 2.0029 CSV PDB
8.0 -0.8149 1.993 CSV PDB
8.5 -0.7658 1.9897 CSV PDB
9.0 -0.7104 1.9886 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH