Volagidemab / 5xez

A4DAB298_5xez_C_D | Source PDB: 5xez | Chains: C, D | Done
99.15
Yes
10 Å
Yes
Avg A4D Score
-0.6864
Max Score
2.8735
Min Score
-3.6984
Total Score
-305.44
Score CDR
-0.2499
Score non-CDR
-0.7479

Calculation Parameters

Chain Sequence(s) QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAVMYYDGSNKDYVDSVKGRFTISRDNSKNTLYLQMNRLRAEDTAVYYCAREKDHYDILTGYNYYYGLDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKRLIYAASSLESGVPSRFSGSGSGTEFTLTISSVQPEDFVTYYCLQHNSNPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) C (Heavy), D (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6984
Maximum Score 2.8735
Average Score -0.6864
Total Score -305.4418

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q C -1.0583 Soluble
2 V C -0.0239 Soluble
3 Q C -0.5776 Soluble
4 L C 0.0000 Buried
5 V C 0.4629 Aggregation-prone
6 E C 0.0000 Buried
7 S C -0.5892 Soluble
8 G C -0.8130 Soluble
9 G C -0.4587 Soluble
11 G C 0.2849 Aggregation-prone
12 V C 1.0117 Aggregation-prone
13 V C 0.0000 Buried
14 Q C -1.4214 Soluble
15 P C -2.1515 Soluble
16 G C -2.6623 Soluble
17 R C -3.0442 Soluble
18 S C -2.3748 Soluble
19 L C -1.5230 Soluble
20 R C -2.1221 Soluble
21 L C 0.0000 Buried
22 S C -0.5577 Soluble
23 C C 0.0000 Buried
24 A C -0.3243 Soluble
25 A C 0.0000 Buried
26 S C -0.5762 Soluble
27 G C -0.5064 Soluble
28 F C -0.1153 Soluble
29 T C -0.1711 Soluble
30 F C 0.0000 Buried
35 S C -0.4907 Soluble
36 S C -0.6472 Soluble
37 Y C 0.0000 Buried
38 G C 0.0000 Buried
39 M C 0.0000 Buried
40 H C 0.0000 Buried
41 W C 0.0000 Buried
42 V C 0.0000 Buried
43 R C 0.0000 Buried
44 Q C -0.8235 Soluble
45 A C -1.2513 Soluble
46 P C -0.9702 Soluble
47 G C -1.4694 Soluble
48 K C -2.2795 Soluble
49 G C -1.4339 Soluble
50 L C 0.0000 Buried
51 E C -1.1134 Soluble
52 W C 0.0000 Buried
53 V C 0.0000 Buried
54 A C 0.0000 Buried
55 V C 0.0000 Buried
56 M C 0.0000 Buried
57 Y C -0.7072 Soluble
58 Y C -0.1624 Soluble
59 D C -1.4229 Soluble
62 G C -1.3064 Soluble
63 S C -1.4307 Soluble
64 N C -2.0301 Soluble
65 K C -1.7342 Soluble
66 D C -1.5818 Soluble
67 Y C -1.3308 Soluble
68 V C -1.4166 Soluble
69 D C -2.4626 Soluble
70 S C -1.7842 Soluble
71 V C 0.0000 Buried
72 K C -2.6280 Soluble
74 G C -1.9037 Soluble
75 R C -1.9548 Soluble
76 F C 0.0000 Buried
77 T C -1.0944 Soluble
78 I C 0.0000 Buried
79 S C -0.4311 Soluble
80 R C -0.8886 Soluble
81 D C -1.6302 Soluble
82 N C -1.6814 Soluble
83 S C -1.7201 Soluble
84 K C -2.4741 Soluble
85 N C -1.8859 Soluble
86 T C 0.0000 Buried
87 L C 0.0000 Buried
88 Y C -0.7009 Soluble
89 L C 0.0000 Buried
90 Q C -1.5007 Soluble
91 M C 0.0000 Buried
92 N C -2.5279 Soluble
93 R C -3.2285 Soluble
94 L C 0.0000 Buried
95 R C -2.6157 Soluble
96 A C -1.5168 Soluble
97 E C -2.1819 Soluble
98 D C 0.0000 Buried
99 T C -0.6389 Soluble
100 A C 0.0000 Buried
101 V C 0.1206 Aggregation-prone
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 A C 0.0000 Buried
106 R C -0.4934 Soluble
107 E C 0.0000 Buried
108 K C -1.3069 Soluble
109 D C -0.5218 Soluble
110 H C -0.0894 Soluble
111 Y C 1.5734 Aggregation-prone
111A D C 1.7681 Aggregation-prone
111B I C 2.8735 Aggregation-prone
111C L C 2.6654 Aggregation-prone
111D T C 1.5760 Aggregation-prone
112D G C 1.3836 Aggregation-prone
112C Y C 1.7202 Aggregation-prone
112B N C 0.8186 Aggregation-prone
112A Y C 0.8905 Aggregation-prone
112 Y C 0.8878 Aggregation-prone
113 Y C 1.0010 Aggregation-prone
114 G C 0.0000 Buried
115 L C 0.0000 Buried
116 D C -0.5493 Soluble
117 V C -0.2714 Soluble
118 W C -0.3062 Soluble
119 G C 0.0000 Buried
120 Q C -1.4231 Soluble
121 G C -0.7391 Soluble
122 T C 0.0000 Buried
123 T C 0.0517 Aggregation-prone
124 V C 0.0000 Buried
125 T C -0.1433 Soluble
126 V C 0.0000 Buried
127 S C -0.2210 Soluble
128 S C -0.3280 Soluble
129 A C -0.2690 Soluble
130 S C -0.3956 Soluble
131 T C -0.5384 Soluble
132 K C -1.1242 Soluble
133 G C -1.3363 Soluble
134 P C 0.0000 Buried
135 S C -0.3455 Soluble
136 V C 0.0000 Buried
137 F C 0.0000 Buried
138 P C -0.9321 Soluble
139 L C 0.0000 Buried
140 A C -1.1255 Soluble
141 P C 0.0000 Buried
142 S C 0.0000 Buried
143 S C -0.8286 Soluble
144 K C -1.2210 Soluble
145 S C 0.0000 Buried
146 T C -0.6905 Soluble
147 S C -0.7073 Soluble
148 G C -0.8067 Soluble
149 G C -0.8726 Soluble
150 T C -0.5711 Soluble
151 A C 0.0000 Buried
152 A C 0.0000 Buried
153 L C 0.0000 Buried
154 G C 0.0000 Buried
155 C C 0.0000 Buried
156 L C 0.0000 Buried
157 V C 0.0000 Buried
158 K C 0.0000 Buried
159 D C -0.4330 Soluble
160 Y C 0.0000 Buried
161 F C 0.0000 Buried
162 P C -0.6369 Soluble
163 E C -1.1486 Soluble
164 P C -1.1310 Soluble
165 V C -0.8758 Soluble
166 T C -0.6640 Soluble
167 V C -0.3591 Soluble
168 S C -0.3901 Soluble
169 W C 0.0000 Buried
170 N C -0.7402 Soluble
171 S C -0.6921 Soluble
172 G C -0.5483 Soluble
173 A C -0.2483 Soluble
174 L C -0.0167 Soluble
175 T C -0.1727 Soluble
176 S C -0.1759 Soluble
177 G C -0.2103 Soluble
178 V C 0.1822 Aggregation-prone
179 H C -0.2823 Soluble
180 T C -0.1520 Soluble
181 F C 0.0000 Buried
182 P C -0.1933 Soluble
183 A C 0.0732 Aggregation-prone
184 V C 0.4475 Aggregation-prone
185 L C 0.9643 Aggregation-prone
186 Q C 0.2126 Aggregation-prone
187 S C -0.0940 Soluble
188 S C -0.2033 Soluble
189 G C 0.0243 Aggregation-prone
190 L C 0.0456 Aggregation-prone
191 Y C 0.2580 Aggregation-prone
192 S C 0.0000 Buried
193 L C 0.0000 Buried
194 S C 0.0000 Buried
195 S C 0.0000 Buried
196 V C 0.0000 Buried
197 V C 0.0000 Buried
198 T C -0.1161 Soluble
199 V C 0.0000 Buried
200 P C -0.4711 Soluble
201 S C -0.5414 Soluble
202 S C -0.5477 Soluble
203 S C -0.5932 Soluble
204 L C -0.6695 Soluble
205 G C -1.0098 Soluble
206 T C -0.8000 Soluble
207 Q C -1.2266 Soluble
208 T C -1.1158 Soluble
209 Y C 0.0000 Buried
210 I C -1.0834 Soluble
211 C C 0.0000 Buried
212 N C -1.4352 Soluble
213 V C 0.0000 Buried
214 N C -1.9768 Soluble
215 H C 0.0000 Buried
216 K C -2.7785 Soluble
217 P C -1.6858 Soluble
218 S C -1.8019 Soluble
219 N C -2.5131 Soluble
220 T C -2.0581 Soluble
221 K C -2.6669 Soluble
222 V C -1.5264 Soluble
223 D C -2.4485 Soluble
224 K C -1.9744 Soluble
225 K C -2.1693 Soluble
226 V C 0.0000 Buried
227 E C -2.4725 Soluble
228 P C -1.6523 Soluble
229 K C -1.8385 Soluble
230 S C -0.8785 Soluble
231 C C -0.0030 Soluble
1 D D -2.4698 Soluble
2 I D 0.0000 Buried
3 Q D -2.0879 Soluble
4 M D 0.0000 Buried
5 T D -0.8840 Soluble
6 Q D -0.7584 Soluble
7 S D -0.5546 Soluble
8 P D -0.5459 Soluble
9 S D -0.7508 Soluble
10 S D -0.6904 Soluble
11 L D -0.4045 Soluble
12 S D -0.7532 Soluble
13 A D 0.0000 Buried
14 S D -0.6174 Soluble
15 V D -0.1364 Soluble
16 G D -0.9038 Soluble
17 D D -1.6667 Soluble
18 R D -2.2997 Soluble
19 V D 0.0000 Buried
20 T D -0.5892 Soluble
21 I D 0.0000 Buried
22 T D -0.7051 Soluble
23 C D 0.0000 Buried
24 R D -1.9724 Soluble
25 A D 0.0000 Buried
26 S D -1.8791 Soluble
27 Q D -2.5398 Soluble
28 G D -2.1811 Soluble
29 I D 0.0000 Buried
36 R D -2.9328 Soluble
37 N D -2.0124 Soluble
38 D D -0.8446 Soluble
39 L D 0.0000 Buried
40 G D 0.0000 Buried
41 W D 0.0000 Buried
42 Y D 0.0000 Buried
43 Q D 0.0000 Buried
44 Q D 0.0000 Buried
45 K D -1.6033 Soluble
46 P D -1.3989 Soluble
47 G D -1.6459 Soluble
48 K D -2.5681 Soluble
49 A D -1.6400 Soluble
50 P D 0.0000 Buried
51 K D -1.6515 Soluble
52 R D 0.0000 Buried
53 L D 0.0000 Buried
54 I D 0.0000 Buried
55 Y D 0.4200 Aggregation-prone
56 A D -0.0286 Soluble
57 A D 0.0000 Buried
65 S D -0.3202 Soluble
66 S D 0.0719 Aggregation-prone
67 L D 0.2451 Aggregation-prone
68 E D -0.4306 Soluble
69 S D -0.4401 Soluble
70 G D -0.5351 Soluble
71 V D -0.2320 Soluble
72 P D -0.3392 Soluble
74 S D -0.4278 Soluble
75 R D -0.8043 Soluble
76 F D 0.0000 Buried
77 S D -0.3140 Soluble
78 G D -0.2068 Soluble
79 S D -0.8200 Soluble
80 G D -1.4952 Soluble
83 S D -1.8580 Soluble
84 G D -2.2753 Soluble
85 T D -2.2289 Soluble
86 E D -2.7643 Soluble
87 F D 0.0000 Buried
88 T D -0.7933 Soluble
89 L D 0.0000 Buried
90 T D -0.6117 Soluble
91 I D 0.0000 Buried
92 S D -1.4127 Soluble
93 S D -1.1549 Soluble
94 V D 0.0000 Buried
95 Q D -0.9942 Soluble
96 P D -1.0157 Soluble
97 E D -1.9385 Soluble
98 D D 0.0000 Buried
99 F D -0.4366 Soluble
100 V D 0.0000 Buried
101 T D -0.5720 Soluble
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 L D 0.0000 Buried
106 Q D 0.0000 Buried
107 H D 0.0000 Buried
108 N D -1.0939 Soluble
109 S D -1.1265 Soluble
114 N D -1.3976 Soluble
115 P D -1.5358 Soluble
116 L D 0.0000 Buried
117 T D -0.9305 Soluble
118 F D -0.3850 Soluble
119 G D 0.0000 Buried
120 G D -1.1665 Soluble
121 G D -0.8729 Soluble
122 T D 0.0000 Buried
123 K D -1.0303 Soluble
124 V D 0.0000 Buried
125 E D 0.0000 Buried
126 I D -0.3995 Soluble
127 K D -1.1372 Soluble
128 R D -1.0650 Soluble
129 T D -0.0458 Soluble
130 V D 0.6349 Aggregation-prone
131 A D 0.1437 Aggregation-prone
132 A D -0.0628 Soluble
133 P D 0.0000 Buried
134 S D -0.1788 Soluble
135 V D 0.0000 Buried
136 F D 0.0000 Buried
137 I D 0.0000 Buried
138 F D 0.0000 Buried
139 P D -1.1199 Soluble
140 P D 0.0000 Buried
141 S D -1.5702 Soluble
142 D D -2.6831 Soluble
143 E D -2.0744 Soluble
144 Q D 0.0000 Buried
145 L D -1.8322 Soluble
146 K D -2.4791 Soluble
147 S D -1.5045 Soluble
148 G D -1.0060 Soluble
149 T D -0.9111 Soluble
150 A D 0.0000 Buried
151 S D 0.0000 Buried
152 V D 0.0000 Buried
153 V D 0.0000 Buried
154 C D 0.0000 Buried
155 L D 0.0000 Buried
156 L D 0.0000 Buried
157 N D 0.0000 Buried
158 N D -0.8853 Soluble
159 F D 0.0000 Buried
160 Y D 0.0000 Buried
161 P D -1.1309 Soluble
162 R D -1.7367 Soluble
163 E D -2.5474 Soluble
164 A D -1.9218 Soluble
165 K D -2.2343 Soluble
166 V D 0.0000 Buried
167 Q D -0.5203 Soluble
168 W D 0.0000 Buried
169 K D -0.5315 Soluble
170 V D 0.0000 Buried
171 D D -1.9591 Soluble
172 N D -1.5415 Soluble
173 A D -0.2378 Soluble
174 L D 0.7705 Aggregation-prone
175 Q D -0.3866 Soluble
176 S D -0.7791 Soluble
177 G D -1.3478 Soluble
178 N D -1.7549 Soluble
179 S D -1.5929 Soluble
180 Q D -1.7076 Soluble
181 E D -2.0882 Soluble
182 S D -0.7511 Soluble
183 V D -0.4428 Soluble
184 T D 0.0000 Buried
185 E D -2.0616 Soluble
186 Q D -1.6423 Soluble
187 D D -1.9158 Soluble
188 S D -1.7589 Soluble
189 K D -2.3611 Soluble
190 D D -1.9958 Soluble
191 S D -1.7468 Soluble
192 T D 0.0000 Buried
193 Y D 0.0000 Buried
194 S D 0.0000 Buried
195 L D 0.0000 Buried
196 S D 0.0000 Buried
197 S D 0.0000 Buried
198 T D -0.8420 Soluble
199 L D 0.0000 Buried
200 T D -0.6616 Soluble
201 L D -0.9957 Soluble
202 S D -1.1710 Soluble
203 K D -2.0668 Soluble
204 A D -2.0235 Soluble
205 D D -3.0079 Soluble
206 Y D 0.0000 Buried
207 E D -3.5927 Soluble
208 K D -3.6984 Soluble
209 H D -3.4038 Soluble
210 K D -3.4039 Soluble
211 V D -2.0577 Soluble
212 Y D 0.0000 Buried
213 A D -0.6898 Soluble
214 C D 0.0000 Buried
215 E D -0.6086 Soluble
216 V D 0.0000 Buried
217 T D -1.1217 Soluble
218 H D 0.0000 Buried
219 Q D -1.5394 Soluble
220 G D -0.4119 Soluble
221 L D -0.2546 Soluble
222 S D -0.4735 Soluble
223 S D -0.4095 Soluble
224 P D -0.5606 Soluble
225 V D 0.0055 Aggregation-prone
226 T D -0.3890 Soluble
227 K D -0.7653 Soluble
228 S D -0.8390 Soluble
229 F D 0.0000 Buried
230 N D -2.5173 Soluble
231 R D -2.6736 Soluble
232 G D -1.9391 Soluble
233 E D -2.5039 Soluble
234 C D -1.3799 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7418 4.0279 CSV PDB
4.5 -0.7842 3.9974 CSV PDB
5.0 -0.8337 3.9679 CSV PDB
5.5 -0.8809 3.9433 CSV PDB
6.0 -0.9145 3.9286 CSV PDB
6.5 -0.9251 3.9237 CSV PDB
7.0 -0.9129 3.9184 CSV PDB
7.5 -0.8859 3.9046 CSV PDB
8.0 -0.8507 3.8856 CSV PDB
8.5 -0.8091 3.8698 CSV PDB
9.0 -0.7608 3.8604 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH