Muromonab / 1sy6

A4DAB173_1sy6_H_L | Source PDB: 1sy6 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7568
Max Score
1.421
Min Score
-3.578
Total Score
-326.93
Score CDR
-0.6718
Score non-CDR
-0.7667

Calculation Parameters

Chain Sequence(s) QVQLQQSGAELARPGASVKMSCKASGYTFTRYTMHWVKQRPGQGLEWIGYINPSRGYTNYNQKFKDKATLTTDKSSSTAYMQLSSLTSEDSAVYYCARYYDDHYCLDYWGQGTTLTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRQIVLTQSPAIMSASPGEKVTMTCSASSSVSYMNWYQQKSGTSPKRWIYDTSKLASGVPAHFRGSGSGTSYSLTISGMEAEDAATYYCQQWSSNPFTFGSGTKLEINRADTAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.578
Maximum Score 1.421
Average Score -0.7568
Total Score -326.9296

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.6031 Soluble
2 V H 0.0000 Buried
3 Q H -2.1599 Soluble
4 L H 0.0000 Buried
5 Q H -2.3745 Soluble
6 Q H 0.0000 Buried
7 S H -1.3047 Soluble
8 G H -1.0846 Soluble
9 A H -0.8343 Soluble
11 E H -1.4438 Soluble
12 L H -0.9168 Soluble
13 A H 0.0000 Buried
14 R H -2.2901 Soluble
15 P H -1.6403 Soluble
16 G H -1.2064 Soluble
17 A H -0.9441 Soluble
18 S H -1.0843 Soluble
19 V H -1.3245 Soluble
20 K H -2.0526 Soluble
21 M H 0.0000 Buried
22 S H -1.0274 Soluble
23 C H 0.0000 Buried
24 K H -2.1607 Soluble
25 A H 0.0000 Buried
26 S H -1.3755 Soluble
27 G H -0.9965 Soluble
28 Y H -0.6770 Soluble
29 T H -0.6404 Soluble
30 F H 0.0000 Buried
35 T H -1.7785 Soluble
36 R H -2.0779 Soluble
37 Y H 0.0000 Buried
38 T H -0.6698 Soluble
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 K H -0.7204 Soluble
44 Q H -1.1274 Soluble
45 R H -1.8336 Soluble
46 P H -1.2480 Soluble
47 G H -1.4448 Soluble
48 Q H -2.0118 Soluble
49 G H -1.2700 Soluble
50 L H 0.0000 Buried
51 E H -0.9931 Soluble
52 W H 0.0000 Buried
53 I H 0.0000 Buried
54 G H 0.0000 Buried
55 Y H 0.0759 Aggregation-prone
56 I H 0.0000 Buried
57 N H -0.9950 Soluble
58 P H 0.0000 Buried
59 S H -1.7462 Soluble
62 R H -2.0753 Soluble
63 G H -1.0464 Soluble
64 Y H -0.1035 Soluble
65 T H -0.1006 Soluble
66 N H -0.5981 Soluble
67 Y H -1.4680 Soluble
68 N H -1.9702 Soluble
69 Q H -3.0186 Soluble
70 K H -3.0750 Soluble
71 F H 0.0000 Buried
72 K H -3.5780 Soluble
74 D H -3.2327 Soluble
75 K H -2.2963 Soluble
76 A H 0.0000 Buried
77 T H -1.2290 Soluble
78 L H 0.0000 Buried
79 T H -0.4359 Soluble
80 T H -1.3423 Soluble
81 D H -1.9363 Soluble
82 K H -2.4891 Soluble
83 S H -1.3658 Soluble
84 S H -1.3240 Soluble
85 S H -1.4644 Soluble
86 T H 0.0000 Buried
87 A H 0.0000 Buried
88 Y H -0.6550 Soluble
89 M H 0.0000 Buried
90 Q H -1.6071 Soluble
91 L H 0.0000 Buried
92 S H -1.1210 Soluble
93 S H -0.9759 Soluble
94 L H 0.0000 Buried
95 T H -1.3257 Soluble
96 S H -1.4736 Soluble
97 E H -2.0750 Soluble
98 D H 0.0000 Buried
99 S H -0.9382 Soluble
100 A H 0.0000 Buried
101 V H -0.0780 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 Y H 0.0000 Buried
108 Y H -1.1083 Soluble
109 D H -2.4762 Soluble
110 D H -2.5255 Soluble
112 H H -1.5623 Soluble
113 Y H -0.5744 Soluble
114 C H 0.0000 Buried
115 L H 0.0000 Buried
116 D H 0.0000 Buried
117 Y H 0.1382 Aggregation-prone
118 W H -0.4247 Soluble
119 G H 0.0000 Buried
120 Q H -1.6200 Soluble
121 G H -0.8631 Soluble
122 T H 0.0000 Buried
123 T H -0.5767 Soluble
124 L H 0.0000 Buried
125 T H -0.8314 Soluble
126 V H 0.0000 Buried
127 S H -1.3811 Soluble
128 S H -1.5626 Soluble
129 A H -1.6187 Soluble
130 K H -2.1595 Soluble
131 T H -1.0655 Soluble
132 T H -0.6275 Soluble
133 A H -0.4304 Soluble
134 P H 0.0000 Buried
135 S H -0.3473 Soluble
136 V H 0.0000 Buried
137 Y H -0.8237 Soluble
138 P H -1.2278 Soluble
139 L H 0.0000 Buried
140 A H 0.0000 Buried
141 P H -0.2379 Soluble
142 V H -0.0227 Soluble
143 C H -0.4563 Soluble
144 G H -0.6469 Soluble
145 G H -0.5599 Soluble
146 T H -0.5051 Soluble
147 T H -0.4531 Soluble
148 G H -0.5193 Soluble
149 S H -0.4619 Soluble
150 S H -0.3216 Soluble
151 V H -0.0155 Soluble
152 T H 0.0680 Aggregation-prone
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.1677 Soluble
159 G H -0.3492 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.4367 Soluble
164 P H -0.4631 Soluble
165 V H -0.1596 Soluble
166 T H -0.2006 Soluble
167 L H -0.0640 Soluble
168 T H -0.3788 Soluble
169 W H 0.0000 Buried
170 N H -1.0177 Soluble
171 S H -0.7519 Soluble
172 G H -0.5155 Soluble
173 S H -0.4393 Soluble
174 L H -0.1678 Soluble
175 S H -0.3524 Soluble
176 S H -0.5042 Soluble
177 G H -0.4003 Soluble
178 V H 0.0964 Aggregation-prone
179 H H -0.1540 Soluble
180 T H 0.0327 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.1854 Soluble
183 A H 0.3020 Aggregation-prone
184 V H 0.7959 Aggregation-prone
185 L H 0.5490 Aggregation-prone
186 Q H -0.8509 Soluble
187 S H -1.1376 Soluble
188 D H -1.9982 Soluble
189 L H -0.7112 Soluble
190 Y H 0.0727 Aggregation-prone
191 T H 0.0000 Buried
192 L H 0.0000 Buried
193 S H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 T H 0.0028 Aggregation-prone
198 V H 0.0389 Aggregation-prone
199 T H -0.2561 Soluble
200 S H -0.4405 Soluble
201 S H -0.6264 Soluble
202 T H -0.6744 Soluble
203 W H 0.0000 Buried
204 P H -1.1776 Soluble
205 S H -1.0209 Soluble
206 Q H -1.4634 Soluble
207 S H -1.3901 Soluble
208 I H 0.0000 Buried
209 T H -1.7378 Soluble
210 C H 0.0000 Buried
211 N H -1.5084 Soluble
212 V H 0.0000 Buried
213 A H -0.8893 Soluble
214 H H 0.0000 Buried
215 P H -0.6804 Soluble
216 A H -0.3569 Soluble
217 S H -0.4399 Soluble
218 S H -0.6993 Soluble
219 T H -0.9041 Soluble
220 K H -2.0162 Soluble
221 V H -1.6355 Soluble
222 D H -2.6255 Soluble
223 K H -2.1639 Soluble
224 K H -2.4997 Soluble
225 I H 0.0000 Buried
226 E H -2.6715 Soluble
227 P H -1.7352 Soluble
228 R H -1.5088 Soluble
1 Q L -1.1395 Soluble
2 I L 0.0000 Buried
3 V L 1.3211 Aggregation-prone
4 L L 0.0000 Buried
5 T L 0.2113 Aggregation-prone
6 Q L 0.0000 Buried
7 S L -0.0611 Soluble
8 P L 0.2539 Aggregation-prone
9 A L 0.5386 Aggregation-prone
10 I L 1.4210 Aggregation-prone
11 M L 0.5842 Aggregation-prone
12 S L -0.3155 Soluble
13 A L 0.0000 Buried
14 S L -1.6087 Soluble
15 P L -1.9499 Soluble
16 G L -2.1898 Soluble
17 E L -2.9947 Soluble
18 K L -2.7458 Soluble
19 V L 0.0000 Buried
20 T L -0.8754 Soluble
21 M L 0.0000 Buried
22 T L -0.2669 Soluble
23 C L 0.0000 Buried
24 S L -0.1604 Soluble
25 A L 0.0000 Buried
26 S L -0.0003 Soluble
27 S L -0.3090 Soluble
28 S L -0.5834 Soluble
29 V L 0.0000 Buried
37 S L -0.5043 Soluble
38 Y L 0.0000 Buried
39 M L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -0.9239 Soluble
45 K L -1.6092 Soluble
46 S L -1.2219 Soluble
47 G L -1.1045 Soluble
48 T L -0.8814 Soluble
49 S L 0.0000 Buried
50 P L 0.0000 Buried
51 K L -0.7216 Soluble
52 R L -0.3330 Soluble
53 W L -0.5728 Soluble
54 I L 0.0000 Buried
55 Y L -0.5758 Soluble
56 D L -1.1847 Soluble
57 T L -1.0990 Soluble
65 S L -1.5828 Soluble
66 K L -1.9499 Soluble
67 L L -0.9327 Soluble
68 A L 0.0000 Buried
69 S L -0.5332 Soluble
70 G L -0.4991 Soluble
71 V L 0.0000 Buried
72 P L -0.6368 Soluble
74 A L -0.4960 Soluble
75 H L -1.0980 Soluble
76 F L 0.0000 Buried
77 R L -2.2082 Soluble
78 G L 0.0000 Buried
79 S L -1.1907 Soluble
80 G L -0.6460 Soluble
83 S L -0.6390 Soluble
84 G L -0.6203 Soluble
85 T L -0.5701 Soluble
86 S L -0.5977 Soluble
87 Y L 0.0000 Buried
88 S L -0.6102 Soluble
89 L L 0.0000 Buried
90 T L -1.1832 Soluble
91 I L 0.0000 Buried
92 S L -1.9085 Soluble
93 G L -2.0516 Soluble
94 M L 0.0000 Buried
95 E L -2.6215 Soluble
96 A L -2.3500 Soluble
97 E L -2.7313 Soluble
98 D L 0.0000 Buried
99 A L -1.4252 Soluble
100 A L -1.2688 Soluble
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 W L -0.0767 Soluble
108 S L -0.3391 Soluble
109 S L -0.8926 Soluble
114 N L -1.3762 Soluble
115 P L -1.1744 Soluble
116 F L 0.0000 Buried
117 T L -0.0949 Soluble
118 F L 0.2867 Aggregation-prone
119 G L 0.0000 Buried
120 S L -0.4350 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -0.8348 Soluble
124 L L 0.0000 Buried
125 E L -0.8287 Soluble
126 I L 0.0000 Buried
127 N L -1.1911 Soluble
128 R L -1.2003 Soluble
129 A L -0.8673 Soluble
130 D L -1.6576 Soluble
131 T L -0.9101 Soluble
132 A L -0.5247 Soluble
133 P L 0.0000 Buried
134 T L -0.0903 Soluble
135 V L 0.0000 Buried
136 S L -0.0283 Soluble
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L 0.0000 Buried
140 P L 0.0000 Buried
141 S L 0.0000 Buried
142 S L -1.1424 Soluble
143 E L -1.8350 Soluble
144 Q L 0.0000 Buried
145 L L -1.0683 Soluble
146 T L -0.8899 Soluble
147 S L -0.8966 Soluble
148 G L -1.2118 Soluble
149 G L -1.0435 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 F L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.6746 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.8775 Soluble
162 K L -2.5505 Soluble
163 D L -2.7741 Soluble
164 I L -1.8326 Soluble
165 N L -2.0091 Soluble
166 V L -0.8897 Soluble
167 K L -1.8775 Soluble
168 W L 0.0000 Buried
169 K L -2.4231 Soluble
170 I L 0.0000 Buried
171 D L -2.9556 Soluble
172 G L -2.1576 Soluble
173 S L -2.2986 Soluble
174 E L -3.3429 Soluble
175 R L -2.8299 Soluble
176 Q L -2.6498 Soluble
177 N L -2.1302 Soluble
178 G L -0.9229 Soluble
179 V L -0.5497 Soluble
180 L L -0.1558 Soluble
181 N L -0.1730 Soluble
182 S L 0.0000 Buried
183 W L -0.1914 Soluble
184 T L -0.9977 Soluble
185 D L -2.2416 Soluble
186 Q L -1.9137 Soluble
187 D L -2.0359 Soluble
188 S L -2.0975 Soluble
189 K L -2.4715 Soluble
190 D L -1.5811 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 M L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L 0.0000 Buried
199 L L 0.0000 Buried
200 T L -0.2134 Soluble
201 L L -0.7435 Soluble
202 T L -1.2451 Soluble
203 K L -2.2367 Soluble
204 D L -3.0845 Soluble
205 E L -2.3678 Soluble
206 Y L 0.0000 Buried
207 E L -3.0714 Soluble
208 R L -3.3016 Soluble
209 H L -2.8995 Soluble
210 N L -3.2774 Soluble
211 S L -2.0375 Soluble
212 Y L 0.0000 Buried
213 T L -1.1659 Soluble
214 C L 0.0000 Buried
215 E L -0.6456 Soluble
216 A L 0.0000 Buried
217 T L -1.0283 Soluble
218 H L 0.0000 Buried
219 K L -2.4986 Soluble
220 T L -1.2194 Soluble
221 S L -0.7974 Soluble
222 T L -0.6169 Soluble
223 S L -0.3273 Soluble
224 P L -0.3087 Soluble
225 I L 0.6581 Aggregation-prone
226 V L 0.7644 Aggregation-prone
227 K L -0.2596 Soluble
228 S L -0.4526 Soluble
229 F L -1.0648 Soluble
230 N L -2.4267 Soluble
231 R L -2.5007 Soluble
232 N L -2.5968 Soluble
233 E L -2.5058 Soluble
234 C L -0.7581 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7891 2.1149 CSV PDB
4.5 -0.8379 2.1105 CSV PDB
5.0 -0.8948 2.1068 CSV PDB
5.5 -0.9504 2.112 CSV PDB
6.0 -0.9934 2.1426 CSV PDB
6.5 -1.0149 2.2189 CSV PDB
7.0 -1.0141 2.3443 CSV PDB
7.5 -0.9976 2.5004 CSV PDB
8.0 -0.9716 2.6697 CSV PDB
8.5 -0.9378 2.8435 CSV PDB
9.0 -0.8952 3.018 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH