Otilimab / 5d71

A4DAB198_5d71_H_L | Source PDB: 5d71 | Chains: H, L | Done
96.86
Yes
10 Å
Yes
Avg A4D Score
-0.6799
Max Score
1.8062
Min Score
-3.1767
Total Score
-278.08
Score CDR
-0.3756
Score non-CDR
-0.7213

Calculation Parameters

Chain Sequence(s) QVQLVESGGGLVQPGGSLRLSCAASGFTFSSYWMNWVRQAPGKGLEWVSGISYSGSETYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARGFGTDFWGQGTLVTVSSASTKGPSVFPLAPSSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPDIELTQPPSVSVAPGQTARISCSGDSIGKKYAYWYQQKPGQAPVLVIERFSGSNSGNTATLTISGTQAEDEADYYCSAWGDKGMVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.1767
Maximum Score 1.8062
Average Score -0.6799
Total Score -278.0776

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.3284 Soluble
2 V H -0.5988 Soluble
3 Q H -0.5917 Soluble
4 L H 0.0000 Buried
5 V H 0.7854 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.2105 Soluble
8 G H -0.6892 Soluble
9 G H 0.1854 Aggregation-prone
11 G H 0.8771 Aggregation-prone
12 L H 1.4588 Aggregation-prone
13 V H 0.0291 Aggregation-prone
14 Q H -1.2259 Soluble
15 P H -1.4628 Soluble
16 G H -1.3333 Soluble
17 G H -0.9379 Soluble
18 S H -1.2551 Soluble
19 L H -0.9957 Soluble
20 R H -2.0742 Soluble
21 L H 0.0000 Buried
22 S H -0.4161 Soluble
23 C H 0.0000 Buried
24 A H -0.1222 Soluble
25 A H 0.0000 Buried
26 S H -0.7579 Soluble
27 G H -0.8662 Soluble
28 F H -0.2837 Soluble
29 T H -0.1760 Soluble
30 F H 0.0000 Buried
35 S H -0.3078 Soluble
36 S H 0.3032 Aggregation-prone
37 Y H 0.9999 Aggregation-prone
38 W H 0.9454 Aggregation-prone
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.6522 Soluble
45 A H -1.0288 Soluble
46 P H -0.7675 Soluble
47 G H -1.4853 Soluble
48 K H -2.2802 Soluble
49 G H -1.4295 Soluble
50 L H 0.0000 Buried
51 E H -0.9384 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 G H 0.0000 Buried
56 I H 0.0000 Buried
57 S H 0.2726 Aggregation-prone
58 Y H 0.8568 Aggregation-prone
59 S H -0.1627 Soluble
62 G H -0.6943 Soluble
63 S H -1.0213 Soluble
64 E H -1.5572 Soluble
65 T H -0.2903 Soluble
66 Y H 0.0808 Aggregation-prone
67 Y H -0.6783 Soluble
68 A H -1.5341 Soluble
69 D H -2.6082 Soluble
70 S H -1.7186 Soluble
71 V H 0.0000 Buried
72 K H -2.4214 Soluble
74 G H -1.7583 Soluble
75 R H -1.5226 Soluble
76 F H 0.0000 Buried
77 T H -0.7152 Soluble
78 I H 0.0000 Buried
79 S H -0.6098 Soluble
80 R H -0.7582 Soluble
81 D H -1.4817 Soluble
82 N H -1.3871 Soluble
83 S H -1.5690 Soluble
84 K H -2.3296 Soluble
85 N H -1.6893 Soluble
86 T H -0.9486 Soluble
87 L H 0.0000 Buried
88 Y H -0.5167 Soluble
89 L H 0.0000 Buried
90 Q H -1.2451 Soluble
91 M H 0.0000 Buried
92 N H -1.4543 Soluble
93 S H -1.1643 Soluble
94 L H 0.0000 Buried
95 R H -2.1250 Soluble
96 A H -1.6609 Soluble
97 E H -2.1799 Soluble
98 D H 0.0000 Buried
99 T H -0.3283 Soluble
100 A H 0.0000 Buried
101 V H 0.7247 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.1865 Aggregation-prone
107 G H 0.0000 Buried
108 F H 1.7507 Aggregation-prone
114 G H 0.4677 Aggregation-prone
115 T H 0.0000 Buried
116 D H 0.0584 Aggregation-prone
117 F H 0.2052 Aggregation-prone
118 W H 0.0000 Buried
119 G H 0.0000 Buried
120 Q H -0.9616 Soluble
121 G H 0.0000 Buried
122 T H 0.5731 Aggregation-prone
123 L H 1.5668 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.4449 Aggregation-prone
126 V H 0.0000 Buried
127 S H -0.6526 Soluble
128 S H -0.6467 Soluble
129 A H -0.4281 Soluble
130 S H -0.6002 Soluble
131 T H -0.9846 Soluble
132 K H -1.6742 Soluble
133 G H -1.5498 Soluble
134 P H 0.0000 Buried
135 S H -0.2817 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.8437 Soluble
139 L H 0.0000 Buried
140 A H -0.8010 Soluble
141 P H 0.0000 Buried
142 S H -0.5839 Soluble
143 S H -0.3708 Soluble
144 G H -0.7678 Soluble
145 G H -0.8736 Soluble
146 T H -0.6497 Soluble
147 A H 0.0000 Buried
148 A H -0.0870 Soluble
149 L H 0.0000 Buried
150 G H 0.0000 Buried
151 C H 0.0000 Buried
152 L H 0.0000 Buried
153 V H 0.0000 Buried
154 K H 0.0000 Buried
155 D H -0.4864 Soluble
156 Y H 0.0000 Buried
157 F H 0.0000 Buried
158 P H -1.5017 Soluble
159 E H -2.1812 Soluble
160 P H -1.6550 Soluble
161 V H -1.0834 Soluble
162 T H -0.7414 Soluble
163 V H -0.2327 Soluble
164 S H -0.3736 Soluble
165 W H 0.0000 Buried
166 N H -0.6213 Soluble
167 S H -0.6382 Soluble
168 G H -0.5563 Soluble
169 A H -0.1999 Soluble
170 L H 0.0880 Aggregation-prone
171 T H -0.1304 Soluble
172 S H -0.2717 Soluble
173 G H -0.3261 Soluble
174 V H 0.1516 Aggregation-prone
175 H H -0.1054 Soluble
176 T H 0.0213 Aggregation-prone
177 F H 0.0000 Buried
178 P H -0.1134 Soluble
179 A H -0.1295 Soluble
180 V H 0.0000 Buried
181 L H 0.2554 Aggregation-prone
182 Q H -0.1622 Soluble
183 S H -0.3277 Soluble
184 S H -0.2831 Soluble
185 G H -0.1194 Soluble
186 L H -0.3249 Soluble
187 Y H -0.2270 Soluble
188 S H 0.0000 Buried
189 L H 0.0000 Buried
190 S H 0.0000 Buried
191 S H 0.0000 Buried
192 V H 0.1753 Aggregation-prone
193 V H 0.0000 Buried
194 T H -0.1888 Soluble
195 V H 0.0000 Buried
196 P H -0.6200 Soluble
197 S H -0.6550 Soluble
198 S H -0.5823 Soluble
199 S H -0.5310 Soluble
200 L H -0.7883 Soluble
201 G H -1.1069 Soluble
202 T H -0.6375 Soluble
203 Q H -1.1409 Soluble
204 T H -0.9202 Soluble
205 Y H 0.0000 Buried
206 I H -0.8290 Soluble
207 C H 0.0000 Buried
208 N H -1.4718 Soluble
209 V H 0.0000 Buried
210 N H -1.9572 Soluble
211 H H 0.0000 Buried
212 K H -2.9319 Soluble
213 P H -1.9209 Soluble
214 S H -1.9095 Soluble
215 N H -2.6405 Soluble
216 T H -2.1151 Soluble
217 K H -2.6586 Soluble
218 V H -1.5548 Soluble
219 D H -2.4213 Soluble
220 K H -1.8039 Soluble
221 K H -1.9280 Soluble
222 V H 0.0000 Buried
223 E H -2.3468 Soluble
224 P H -1.2434 Soluble
1 D L -3.0744 Soluble
2 I L -2.4490 Soluble
3 E L -2.4958 Soluble
4 L L 0.0000 Buried
5 T L -0.6655 Soluble
6 Q L 0.0000 Buried
7 P L -0.5310 Soluble
8 P L -0.7848 Soluble
9 S L -1.0035 Soluble
11 V L -0.7933 Soluble
12 S L -0.6000 Soluble
13 V L 0.0000 Buried
14 A L -0.7214 Soluble
15 P L -1.3036 Soluble
16 G L -1.4168 Soluble
17 Q L -1.8901 Soluble
18 T L -1.4315 Soluble
19 A L 0.0000 Buried
20 R L -2.0398 Soluble
21 I L 0.0000 Buried
22 S L -0.6945 Soluble
23 C L 0.0000 Buried
24 S L -1.4017 Soluble
25 G L -2.2246 Soluble
26 D L -3.1277 Soluble
27 S L -2.3075 Soluble
28 I L 0.0000 Buried
29 G L -2.1140 Soluble
30 K L -2.4088 Soluble
31 K L -0.9276 Soluble
32 Y L 0.5695 Aggregation-prone
33 A L 0.0855 Aggregation-prone
34 Y L 0.0000 Buried
35 W L 0.0000 Buried
36 Y L 0.0000 Buried
37 Q L -0.0733 Soluble
38 Q L -0.7868 Soluble
39 K L -1.5937 Soluble
40 P L -1.1111 Soluble
41 G L -1.2550 Soluble
42 Q L -1.3813 Soluble
43 A L 0.0000 Buried
44 P L 0.0000 Buried
45 V L 1.1612 Aggregation-prone
46 L L 1.0803 Aggregation-prone
47 V L 1.3884 Aggregation-prone
48 I L 1.8062 Aggregation-prone
60 E L -1.7655 Soluble
61 R L -1.2443 Soluble
62 F L -0.5478 Soluble
63 S L -0.7412 Soluble
64 G L -0.6644 Soluble
65 S L -0.8033 Soluble
66 N L -1.3116 Soluble
67 S L -1.5590 Soluble
68 G L -2.0298 Soluble
69 N L -2.6018 Soluble
70 T L -1.4172 Soluble
71 A L 0.0000 Buried
72 T L -0.9115 Soluble
73 L L 0.0000 Buried
74 T L -1.0760 Soluble
75 I L 0.0000 Buried
76 S L -1.4284 Soluble
77 G L -1.1177 Soluble
78 T L 0.0000 Buried
79 Q L -1.7728 Soluble
80 A L -1.5274 Soluble
81 E L -2.3767 Soluble
82 D L 0.0000 Buried
83 E L -1.9642 Soluble
84 A L 0.0000 Buried
85 D L 0.0000 Buried
86 Y L 0.0000 Buried
87 Y L 0.0000 Buried
88 C L 0.0000 Buried
89 S L 0.0000 Buried
90 A L 0.0000 Buried
91 W L -0.2435 Soluble
92 G L -2.0876 Soluble
93 D L -2.9968 Soluble
94 K L -2.8891 Soluble
95 G L -1.3717 Soluble
96 M L 0.0000 Buried
97 V L -0.7647 Soluble
98 F L -0.2006 Soluble
99 G L 0.0000 Buried
100 G L -1.0415 Soluble
101 G L 0.0000 Buried
102 T L 0.0000 Buried
103 K L -1.9833 Soluble
104 L L 0.0000 Buried
105 T L -0.6926 Soluble
106 V L 0.0000 Buried
106A L L -0.4229 Soluble
107 G L -0.7653 Soluble
108 Q L -0.9575 Soluble
109 P L -0.9287 Soluble
110 K L -1.5132 Soluble
111 A L -0.9665 Soluble
112 A L -0.5815 Soluble
113 P L 0.0000 Buried
114 S L -0.6197 Soluble
115 V L 0.0000 Buried
116 T L -0.5375 Soluble
117 L L 0.0000 Buried
118 F L 0.0000 Buried
119 P L -0.1026 Soluble
120 P L 0.0000 Buried
121 S L -0.7268 Soluble
122 S L -1.2159 Soluble
123 E L -1.8148 Soluble
124 E L 0.0000 Buried
125 L L -1.6090 Soluble
126 Q L -2.0539 Soluble
127 A L -1.6402 Soluble
128 N L -2.3596 Soluble
129 K L -2.1179 Soluble
130 A L 0.0000 Buried
131 T L 0.0000 Buried
132 L L 0.0000 Buried
133 V L 0.0000 Buried
134 C L 0.0000 Buried
135 L L 0.0000 Buried
136 I L 0.0000 Buried
137 S L -0.6440 Soluble
138 D L -1.4530 Soluble
139 F L 0.0000 Buried
140 Y L 0.0000 Buried
141 P L 0.0000 Buried
142 G L -0.9015 Soluble
143 A L -0.5161 Soluble
144 V L -0.0762 Soluble
145 T L -0.0690 Soluble
146 V L 0.0563 Aggregation-prone
147 A L -0.4511 Soluble
148 W L 0.0000 Buried
149 K L -0.8268 Soluble
150 A L 0.0000 Buried
151 D L -1.1579 Soluble
152 S L -0.6061 Soluble
153 S L -0.6826 Soluble
154 P L -0.7816 Soluble
155 V L -0.8259 Soluble
156 K L -1.7381 Soluble
157 A L -0.9092 Soluble
158 G L -0.8318 Soluble
159 V L -0.6907 Soluble
160 E L -0.7791 Soluble
161 T L -0.2015 Soluble
162 T L 0.0000 Buried
163 T L -0.4375 Soluble
164 P L -0.4675 Soluble
165 S L -1.0394 Soluble
166 K L -2.2829 Soluble
167 Q L -1.9132 Soluble
168 S L -1.7299 Soluble
169 N L -2.4094 Soluble
170 N L -2.6761 Soluble
171 K L -2.1666 Soluble
172 Y L -1.2947 Soluble
173 A L 0.0000 Buried
174 A L 0.0000 Buried
175 S L 0.0000 Buried
176 S L 0.0000 Buried
177 Y L 0.0000 Buried
178 L L 0.0000 Buried
179 S L -0.4572 Soluble
180 L L -1.0284 Soluble
181 T L -1.6669 Soluble
182 P L 0.0000 Buried
183 E L -3.1531 Soluble
184 Q L -2.5153 Soluble
185 W L 0.0000 Buried
186 K L -3.1767 Soluble
187 S L -2.4545 Soluble
188 H L -2.3828 Soluble
189 R L -2.4581 Soluble
190 S L -1.4571 Soluble
191 Y L 0.0000 Buried
192 S L -1.0625 Soluble
193 C L 0.0000 Buried
194 Q L -1.0787 Soluble
195 V L 0.0000 Buried
196 T L -0.6627 Soluble
197 H L 0.0000 Buried
198 E L -2.1795 Soluble
199 G L -1.4391 Soluble
200 S L -0.8875 Soluble
201 T L -0.7319 Soluble
202 V L -0.7677 Soluble
203 E L -2.2501 Soluble
204 K L -2.0005 Soluble
205 T L -1.0735 Soluble
206 V L -0.3965 Soluble
207 A L -0.8024 Soluble
208 P L -1.0329 Soluble
209 T L -0.5424 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.5841 4.3062 CSV PDB
4.5 -0.6266 4.2699 CSV PDB
5.0 -0.6769 4.2327 CSV PDB
5.5 -0.7248 4.1952 CSV PDB
6.0 -0.7584 4.1577 CSV PDB
6.5 -0.7681 4.1205 CSV PDB
7.0 -0.7536 4.0843 CSV PDB
7.5 -0.7233 4.0508 CSV PDB
8.0 -0.6845 4.0237 CSV PDB
8.5 -0.6401 4.0065 CSV PDB
9.0 -0.5909 3.9984 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH