Vilastobart / 8g8n

A4DAB294_8g8n_K_M | Source PDB: 8g8n | Chains: K, M | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7924
Max Score
1.2823
Min Score
-3.6859
Total Score
-338.38
Score CDR
-0.6165
Score non-CDR
-0.8132

Calculation Parameters

Chain Sequence(s) QVQLVQSGAEVKKPGSSVKVSCKASGYTFTNYFMNWVRQAPGQGLEWMGRVDPEQGRADYAEKFKKRVTITADKSTSTAYMELSSLRSEDTAVYYCARRAMDNYGFAYWGQGTLVTVSSASTKGPSVFPLAPSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPEIVLTQSPDFQSVTPKEKVTITCSANSALSYMYWYQQKPDQSPKLWVHGTSNLASGVPSRFSGSGSGTDFTLTINSLEAEDAATYYCHHWSNTQWTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) K (Heavy), M (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6859
Maximum Score 1.2823
Average Score -0.7924
Total Score -338.3758

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q K -1.4989 Soluble
2 V K -0.9255 Soluble
3 Q K -1.2800 Soluble
4 L K 0.0000 Buried
5 V K -0.2290 Soluble
6 Q K 0.0000 Buried
7 S K -0.7453 Soluble
8 G K -0.9796 Soluble
9 A K -0.8413 Soluble
11 E K -1.1875 Soluble
12 V K -0.6892 Soluble
13 K K -1.5292 Soluble
14 K K -2.3643 Soluble
15 P K -2.1053 Soluble
16 G K -1.5127 Soluble
17 S K -1.2234 Soluble
18 S K -1.3682 Soluble
19 V K 0.0000 Buried
20 K K -2.0262 Soluble
21 V K 0.0000 Buried
22 S K -0.6692 Soluble
23 C K 0.0000 Buried
24 K K -1.2392 Soluble
25 A K 0.0000 Buried
26 S K -0.9498 Soluble
27 G K -1.1179 Soluble
28 Y K -0.6609 Soluble
29 T K -0.5863 Soluble
30 F K 0.0000 Buried
35 T K -1.8821 Soluble
36 N K -1.4987 Soluble
37 Y K -0.5845 Soluble
38 F K -0.3897 Soluble
39 M K 0.0000 Buried
40 N K 0.0000 Buried
41 W K 0.0000 Buried
42 V K 0.0000 Buried
43 R K -0.4893 Soluble
44 Q K -0.8042 Soluble
45 A K -1.0745 Soluble
46 P K -1.0269 Soluble
47 G K -1.2331 Soluble
48 Q K -1.7214 Soluble
49 G K -1.1415 Soluble
50 L K 0.0000 Buried
51 E K -0.7213 Soluble
52 W K 0.0000 Buried
53 M K 0.0000 Buried
54 G K 0.0000 Buried
55 R K -0.5651 Soluble
56 V K 0.0000 Buried
57 D K -1.8449 Soluble
58 P K 0.0000 Buried
59 E K -3.3493 Soluble
62 Q K -2.7071 Soluble
63 G K -2.6763 Soluble
64 R K -2.7359 Soluble
65 A K -1.3673 Soluble
66 D K -1.4251 Soluble
67 Y K -1.5592 Soluble
68 A K 0.0000 Buried
69 E K -3.4812 Soluble
70 K K -3.3374 Soluble
71 F K -2.7437 Soluble
72 K K -3.6859 Soluble
74 K K -3.3488 Soluble
75 R K -2.2134 Soluble
76 V K 0.0000 Buried
77 T K -1.1118 Soluble
78 I K 0.0000 Buried
79 T K -0.9625 Soluble
80 A K -1.9607 Soluble
81 D K -2.6119 Soluble
82 K K -2.9560 Soluble
83 S K -1.5186 Soluble
84 T K -1.1408 Soluble
85 S K -1.4959 Soluble
86 T K -1.2774 Soluble
87 A K 0.0000 Buried
88 Y K -0.7303 Soluble
89 M K 0.0000 Buried
90 E K -1.3606 Soluble
91 L K 0.0000 Buried
92 S K -1.3029 Soluble
93 S K -1.1147 Soluble
94 L K 0.0000 Buried
95 R K -2.6595 Soluble
96 S K -2.1752 Soluble
97 E K -2.4094 Soluble
98 D K 0.0000 Buried
99 T K -0.9046 Soluble
100 A K 0.0000 Buried
101 V K -0.2540 Soluble
102 Y K 0.0000 Buried
103 Y K 0.0000 Buried
104 C K 0.0000 Buried
105 A K 0.0000 Buried
106 R K 0.0000 Buried
107 R K -0.1057 Soluble
108 A K -0.1551 Soluble
109 M K -0.2780 Soluble
110 D K -1.4603 Soluble
112 N K -0.4465 Soluble
113 Y K 0.9065 Aggregation-prone
114 G K 0.0000 Buried
115 F K 0.0000 Buried
116 A K -0.0857 Soluble
117 Y K -0.0502 Soluble
118 W K -0.3001 Soluble
119 G K 0.0000 Buried
120 Q K -1.3561 Soluble
121 G K -0.8634 Soluble
122 T K 0.0000 Buried
123 L K -0.1439 Soluble
124 V K 0.0000 Buried
125 T K 0.0000 Buried
126 V K 0.0000 Buried
127 S K -1.3961 Soluble
128 S K -1.1466 Soluble
129 A K -0.5987 Soluble
130 S K -0.7249 Soluble
131 T K -0.9964 Soluble
132 K K -1.9258 Soluble
133 G K -1.7010 Soluble
134 P K -0.8247 Soluble
135 S K -0.4125 Soluble
136 V K 0.0000 Buried
137 F K 0.0000 Buried
138 P K -1.0228 Soluble
139 L K 0.0000 Buried
140 A K -0.7805 Soluble
141 P K 0.0000 Buried
142 S K -0.2427 Soluble
143 G K -0.8249 Soluble
144 G K -0.8594 Soluble
145 T K -0.5649 Soluble
146 A K -0.2648 Soluble
147 A K 0.0000 Buried
148 L K 0.0000 Buried
149 G K 0.0000 Buried
150 C K 0.0000 Buried
151 L K 0.0000 Buried
152 V K 0.0000 Buried
153 K K 0.0000 Buried
154 D K -0.7203 Soluble
155 Y K 0.0000 Buried
156 F K -0.5128 Soluble
157 P K 0.0000 Buried
158 E K -0.3451 Soluble
159 P K -0.5910 Soluble
160 V K -0.6278 Soluble
161 T K -0.5766 Soluble
162 V K -0.3724 Soluble
163 S K -0.3532 Soluble
164 W K 0.0000 Buried
165 N K -0.6121 Soluble
166 S K -0.5655 Soluble
167 G K -0.4261 Soluble
168 A K -0.1386 Soluble
169 L K 0.1201 Aggregation-prone
170 T K -0.1224 Soluble
171 S K -0.4766 Soluble
172 G K -0.4590 Soluble
173 V K 0.1485 Aggregation-prone
174 H K 0.0000 Buried
175 T K 0.0862 Aggregation-prone
176 F K 0.0000 Buried
177 P K -0.2110 Soluble
178 A K 0.2222 Aggregation-prone
179 V K 0.3451 Aggregation-prone
180 L K 0.9016 Aggregation-prone
181 Q K 0.1339 Aggregation-prone
182 S K -0.1665 Soluble
183 S K -0.2699 Soluble
184 G K 0.0243 Aggregation-prone
185 L K -0.0390 Soluble
186 Y K 0.2494 Aggregation-prone
187 S K 0.0000 Buried
188 L K 0.0000 Buried
189 S K 0.0000 Buried
190 S K 0.0000 Buried
191 V K 0.0000 Buried
192 V K 0.0000 Buried
193 T K -0.0884 Soluble
194 V K 0.0000 Buried
195 P K -0.6298 Soluble
196 S K -0.6134 Soluble
197 S K -0.6505 Soluble
198 S K 0.0000 Buried
199 L K -0.7088 Soluble
200 G K -0.9081 Soluble
201 T K -0.6049 Soluble
202 Q K -0.9929 Soluble
203 T K -0.9148 Soluble
204 Y K 0.0000 Buried
205 I K -0.8233 Soluble
206 C K 0.0000 Buried
207 N K -1.3981 Soluble
208 V K 0.0000 Buried
209 N K -1.9851 Soluble
210 H K 0.0000 Buried
211 K K -2.8631 Soluble
212 P K -1.7766 Soluble
213 S K -1.9502 Soluble
214 N K -2.6586 Soluble
215 T K -2.1691 Soluble
216 K K -2.7380 Soluble
217 V K -1.5937 Soluble
218 D K -2.3795 Soluble
219 K K -1.8237 Soluble
220 K K -1.8320 Soluble
221 V K 0.0000 Buried
222 E K -2.2601 Soluble
223 P K -1.1147 Soluble
1 E M -1.7338 Soluble
2 I M -0.3113 Soluble
3 V M 1.0544 Aggregation-prone
4 L M 0.0000 Buried
5 T M -0.3425 Soluble
6 Q M 0.0000 Buried
7 S M -0.6182 Soluble
8 P M -0.6819 Soluble
9 D M -1.3350 Soluble
10 F M 0.4279 Aggregation-prone
11 Q M -0.4093 Soluble
12 S M -0.5042 Soluble
13 V M 0.0000 Buried
14 T M -1.9204 Soluble
15 P M -2.5004 Soluble
16 K M -3.2937 Soluble
17 E M -3.5041 Soluble
18 K M -3.1469 Soluble
19 V M 0.0000 Buried
20 T M -0.6885 Soluble
21 I M 0.0000 Buried
22 T M -0.5723 Soluble
23 C M 0.0000 Buried
24 S M -0.8748 Soluble
25 A M -0.7261 Soluble
26 N M -1.3149 Soluble
27 S M -0.8242 Soluble
28 A M -0.5926 Soluble
29 L M -0.0690 Soluble
37 S M 0.4265 Aggregation-prone
38 Y M 1.2823 Aggregation-prone
39 M M 0.0000 Buried
40 Y M 0.0000 Buried
41 W M 0.0000 Buried
42 Y M 0.0000 Buried
43 Q M 0.0000 Buried
44 Q M 0.0000 Buried
45 K M -1.8768 Soluble
46 P M -1.9447 Soluble
47 D M -2.5658 Soluble
48 Q M -2.5002 Soluble
49 S M -1.5966 Soluble
50 P M 0.0000 Buried
51 K M -1.7489 Soluble
52 L M 0.0000 Buried
53 W M 0.0000 Buried
54 V M 0.0000 Buried
55 H M -0.0871 Soluble
56 G M 0.1257 Aggregation-prone
57 T M -0.1619 Soluble
65 S M -0.6147 Soluble
66 N M -1.0127 Soluble
67 L M -0.3396 Soluble
68 A M -0.5031 Soluble
69 S M -0.3970 Soluble
70 G M -0.4587 Soluble
71 V M -0.4328 Soluble
72 P M -0.3085 Soluble
74 S M -0.5187 Soluble
75 R M -1.1429 Soluble
76 F M 0.0000 Buried
77 S M -0.6254 Soluble
78 G M -0.5573 Soluble
79 S M -0.7018 Soluble
80 G M -0.9342 Soluble
83 S M -0.8569 Soluble
84 G M -1.0330 Soluble
85 T M -1.4043 Soluble
86 D M -2.1857 Soluble
87 F M 0.0000 Buried
88 T M -0.6602 Soluble
89 L M 0.0000 Buried
90 T M -0.7831 Soluble
91 I M 0.0000 Buried
92 N M -2.6651 Soluble
93 S M -2.8354 Soluble
94 L M 0.0000 Buried
95 E M -2.9390 Soluble
96 A M -2.6689 Soluble
97 E M -2.8963 Soluble
98 D M 0.0000 Buried
99 A M -1.6198 Soluble
100 A M 0.0000 Buried
101 T M -0.5343 Soluble
102 Y M 0.0000 Buried
103 Y M 0.0000 Buried
104 C M 0.0000 Buried
105 H M 0.0000 Buried
106 H M 0.0000 Buried
107 W M 0.3282 Aggregation-prone
108 S M -0.7032 Soluble
109 N M -1.6441 Soluble
114 T M -1.0809 Soluble
115 Q M -1.2499 Soluble
116 W M 0.0000 Buried
117 T M 0.1108 Aggregation-prone
118 F M 0.2635 Aggregation-prone
119 G M 0.0000 Buried
120 G M -0.9588 Soluble
121 G M -0.8211 Soluble
122 T M 0.0000 Buried
123 K M -0.6911 Soluble
124 V M 0.0000 Buried
125 E M -1.1724 Soluble
126 I M 0.0000 Buried
127 K M -1.3675 Soluble
128 R M -0.9253 Soluble
129 T M 0.2128 Aggregation-prone
130 V M 0.9108 Aggregation-prone
131 A M 0.3870 Aggregation-prone
132 A M 0.0121 Aggregation-prone
133 P M -0.0569 Soluble
134 S M -0.0376 Soluble
135 V M 0.1457 Aggregation-prone
136 F M 0.3660 Aggregation-prone
137 I M 0.2241 Aggregation-prone
138 F M 0.0000 Buried
139 P M -0.8021 Soluble
140 P M -0.9883 Soluble
141 S M -1.6068 Soluble
142 D M -2.8888 Soluble
143 E M -2.8099 Soluble
144 Q M 0.0000 Buried
145 L M -2.2517 Soluble
146 K M -2.8018 Soluble
147 S M -1.7943 Soluble
148 G M -1.3094 Soluble
149 T M -1.2733 Soluble
150 A M 0.0000 Buried
151 S M 0.0000 Buried
152 V M 0.0000 Buried
153 V M 0.0000 Buried
154 C M 0.0000 Buried
155 L M 0.0000 Buried
156 L M 0.0000 Buried
157 N M 0.0000 Buried
158 N M -0.9555 Soluble
159 F M 0.0000 Buried
160 Y M 0.0000 Buried
161 P M -1.6653 Soluble
162 R M -2.9060 Soluble
163 E M -3.2430 Soluble
164 A M -2.3863 Soluble
165 K M -2.3357 Soluble
166 V M -1.2365 Soluble
167 Q M -0.8647 Soluble
168 W M 0.0000 Buried
169 K M -0.6711 Soluble
170 V M 0.0000 Buried
171 D M -1.9470 Soluble
172 N M -1.5247 Soluble
173 A M -0.2746 Soluble
174 L M 0.6651 Aggregation-prone
175 Q M -0.2137 Soluble
176 S M -0.4766 Soluble
177 G M -0.9795 Soluble
178 N M -0.9586 Soluble
179 S M -1.1269 Soluble
180 Q M -1.2802 Soluble
181 E M -1.4638 Soluble
182 S M 0.0000 Buried
183 V M -0.6132 Soluble
184 T M -0.9974 Soluble
185 E M -2.1253 Soluble
186 Q M -2.0954 Soluble
187 D M -2.1319 Soluble
188 S M -2.4446 Soluble
189 K M -2.8653 Soluble
190 D M -2.0360 Soluble
191 S M 0.0000 Buried
192 T M 0.0000 Buried
193 Y M 0.0000 Buried
194 S M 0.0000 Buried
195 L M 0.0000 Buried
196 S M 0.0000 Buried
197 S M 0.0000 Buried
198 T M -0.5419 Soluble
199 L M 0.0000 Buried
200 T M -0.5073 Soluble
201 L M -0.6991 Soluble
202 S M -1.1411 Soluble
203 K M -2.1036 Soluble
204 A M -1.9180 Soluble
205 D M -2.7958 Soluble
206 Y M 0.0000 Buried
207 E M -3.0083 Soluble
208 K M -3.3939 Soluble
209 H M -2.9489 Soluble
210 K M -2.9853 Soluble
211 V M -1.5988 Soluble
212 Y M 0.0000 Buried
213 A M 0.0000 Buried
214 C M 0.0000 Buried
215 E M -0.9577 Soluble
216 V M 0.0000 Buried
217 T M -1.2887 Soluble
218 H M 0.0000 Buried
219 Q M -1.7437 Soluble
220 G M -0.2970 Soluble
221 L M -0.1838 Soluble
222 S M -0.4565 Soluble
223 S M -0.4434 Soluble
224 P M -0.6300 Soluble
225 V M -0.0141 Soluble
226 T M -0.4833 Soluble
227 K M -0.6890 Soluble
228 S M -0.6389 Soluble
229 F M -1.1044 Soluble
230 N M -2.1060 Soluble
231 R M -2.3160 Soluble
232 G M -1.9234 Soluble
233 E M -2.0574 Soluble
234 C M -0.4732 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.774 3.3512 CSV PDB
4.5 -0.8295 3.1677 CSV PDB
5.0 -0.8945 2.9591 CSV PDB
5.5 -0.9554 2.7446 CSV PDB
6.0 -0.9969 2.5385 CSV PDB
6.5 -1.0075 2.3532 CSV PDB
7.0 -0.9878 2.1929 CSV PDB
7.5 -0.9489 2.0516 CSV PDB
8.0 -0.9001 1.9264 CSV PDB
8.5 -0.8445 1.8276 CSV PDB
9.0 -0.7818 1.7766 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH