Sabestomig / 8tft

A4DAB233_8tft_H_L | Source PDB: 8tft | Chains: H, L | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.6867
Max Score
1.4776
Min Score
-3.2835
Total Score
-300.09
Score CDR
-0.3943
Score non-CDR
-0.7253

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARGSYGTYYGNYFEYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSSYVLTQPPSVSVAPGKTARITCGGDNIGGKSVHWYQQKPGQAPVLVIYYDSDRPSGIPERFSGSNSGNTATLTISRVEAGDEADYYCQVLDRRSDHWLFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.2835
Maximum Score 1.4776
Average Score -0.6867
Total Score -300.0934

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0383 Soluble
2 V H -1.0734 Soluble
3 Q H -1.0569 Soluble
4 L H 0.0000 Buried
5 L H 0.4804 Aggregation-prone
6 E H -0.0187 Soluble
7 S H -0.5698 Soluble
8 G H -0.9612 Soluble
9 G H -0.4238 Soluble
11 G H -0.3867 Soluble
12 L H 0.0951 Aggregation-prone
13 V H -0.5996 Soluble
14 Q H -1.6260 Soluble
15 P H -1.8036 Soluble
16 G H -1.3934 Soluble
17 G H -1.0490 Soluble
18 S H -1.1807 Soluble
19 L H -0.9580 Soluble
20 R H -2.0305 Soluble
21 L H 0.0000 Buried
22 S H -0.4756 Soluble
23 C H 0.0000 Buried
24 A H -0.1785 Soluble
25 A H 0.0000 Buried
26 S H -0.8763 Soluble
27 G H -1.1383 Soluble
28 F H -0.4389 Soluble
29 T H -0.2086 Soluble
30 F H 0.0000 Buried
35 S H -0.5819 Soluble
36 S H 0.1263 Aggregation-prone
37 Y H 0.4861 Aggregation-prone
38 A H 0.0000 Buried
39 M H 0.0000 Buried
40 S H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8892 Soluble
45 A H -1.2158 Soluble
46 P H -0.9703 Soluble
47 G H -1.4869 Soluble
48 K H -2.3102 Soluble
49 G H -1.5528 Soluble
50 L H 0.0000 Buried
51 E H -1.2143 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 A H 0.0000 Buried
56 I H 0.0000 Buried
57 S H 0.0000 Buried
58 G H -0.0848 Soluble
59 S H -0.5608 Soluble
62 G H -0.7953 Soluble
63 G H -0.5473 Soluble
64 S H -0.1743 Soluble
65 T H -0.1547 Soluble
66 Y H -0.3520 Soluble
67 Y H -0.9399 Soluble
68 A H 0.0000 Buried
69 D H -2.5032 Soluble
70 S H -1.5937 Soluble
71 V H 0.0000 Buried
72 K H -2.5168 Soluble
74 G H -1.7222 Soluble
75 R H -1.2914 Soluble
76 F H 0.0000 Buried
77 T H -0.7473 Soluble
78 I H 0.0000 Buried
79 S H -0.4418 Soluble
80 R H -1.0084 Soluble
81 D H -1.6558 Soluble
82 N H -1.7740 Soluble
83 S H -1.6137 Soluble
84 K H -2.3795 Soluble
85 N H -1.7177 Soluble
86 T H -1.0279 Soluble
87 L H 0.0000 Buried
88 Y H -0.4064 Soluble
89 L H 0.0000 Buried
90 Q H -1.0797 Soluble
91 M H 0.0000 Buried
92 N H -1.3706 Soluble
93 S H -1.2246 Soluble
94 L H 0.0000 Buried
95 R H -2.4179 Soluble
96 A H -1.8038 Soluble
97 E H -2.2490 Soluble
98 D H 0.0000 Buried
99 T H -0.7320 Soluble
100 A H 0.0000 Buried
101 V H 0.2394 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 G H 0.0000 Buried
108 S H 0.6627 Aggregation-prone
109 Y H 1.1966 Aggregation-prone
110 G H 0.4941 Aggregation-prone
111 T H 0.8092 Aggregation-prone
111A Y H 1.3570 Aggregation-prone
112A Y H 0.4586 Aggregation-prone
112 G H 0.0000 Buried
113 N H 0.1456 Aggregation-prone
114 Y H 0.0000 Buried
115 F H 0.0000 Buried
116 E H 0.1878 Aggregation-prone
117 Y H 0.4939 Aggregation-prone
118 W H 0.2135 Aggregation-prone
119 G H 0.0000 Buried
120 Q H -1.3407 Soluble
121 G H -0.4862 Soluble
122 T H -0.0393 Soluble
123 L H 0.3888 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.3673 Soluble
126 V H 0.0000 Buried
127 S H -0.9786 Soluble
128 S H -0.6236 Soluble
129 A H -0.4258 Soluble
130 S H -0.5687 Soluble
131 T H -0.7002 Soluble
132 K H -1.1667 Soluble
133 G H -1.3888 Soluble
134 P H -0.6410 Soluble
135 S H -0.3402 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.9805 Soluble
139 L H 0.0000 Buried
140 A H -0.9926 Soluble
141 P H -0.6995 Soluble
142 S H -0.8398 Soluble
143 S H -0.8841 Soluble
144 K H -1.1493 Soluble
145 S H -0.8204 Soluble
146 T H -0.7124 Soluble
147 S H -0.6668 Soluble
148 G H -0.9030 Soluble
149 G H -0.8732 Soluble
150 T H -0.6759 Soluble
151 A H 0.0000 Buried
152 A H -0.1377 Soluble
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H -0.3579 Soluble
159 D H -0.4723 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H 0.0000 Buried
163 E H -0.4487 Soluble
164 P H -0.7198 Soluble
165 V H -0.6391 Soluble
166 T H -0.5240 Soluble
167 V H -0.3148 Soluble
168 S H -0.4109 Soluble
169 W H 0.0000 Buried
170 N H -0.8604 Soluble
171 S H -0.7184 Soluble
172 G H -0.5748 Soluble
173 A H -0.2466 Soluble
174 L H -0.0161 Soluble
175 T H -0.1999 Soluble
176 S H -0.2671 Soluble
177 G H -0.3314 Soluble
178 V H 0.2015 Aggregation-prone
179 H H -0.1007 Soluble
180 T H 0.0403 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.0013 Soluble
183 A H 0.2822 Aggregation-prone
184 V H 0.0000 Buried
185 L H 1.2763 Aggregation-prone
186 Q H 0.3324 Aggregation-prone
187 S H -0.0895 Soluble
188 S H -0.1757 Soluble
189 G H 0.0622 Aggregation-prone
190 L H 0.1792 Aggregation-prone
191 Y H 0.3806 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.1935 Aggregation-prone
197 V H 0.0000 Buried
198 T H -0.2189 Soluble
199 V H 0.0000 Buried
200 P H -0.6937 Soluble
201 S H -0.7177 Soluble
202 S H -0.6272 Soluble
203 S H -0.6314 Soluble
204 L H -0.7875 Soluble
205 G H -1.0532 Soluble
206 T H -0.7242 Soluble
207 Q H -1.2631 Soluble
208 T H -1.1912 Soluble
209 Y H 0.0000 Buried
210 I H -1.4183 Soluble
211 C H 0.0000 Buried
212 N H -1.5419 Soluble
213 V H 0.0000 Buried
214 N H -1.7825 Soluble
215 H H 0.0000 Buried
216 K H -2.7415 Soluble
217 P H -1.5521 Soluble
218 S H -1.7755 Soluble
219 N H -2.5407 Soluble
220 T H -2.0320 Soluble
221 K H -2.6500 Soluble
222 V H -1.6085 Soluble
223 D H -2.5928 Soluble
224 K H -2.2823 Soluble
225 R H -2.7344 Soluble
226 V H 0.0000 Buried
227 E H -2.6653 Soluble
228 P H -1.4405 Soluble
229 K H -1.3896 Soluble
230 S H -0.9706 Soluble
1 S L 0.1483 Aggregation-prone
2 Y L 0.3889 Aggregation-prone
3 V L 1.4776 Aggregation-prone
4 L L 0.0000 Buried
5 T L 0.1228 Aggregation-prone
6 Q L -0.4478 Soluble
7 P L -0.6209 Soluble
8 P L -0.9941 Soluble
9 S L -1.0195 Soluble
11 V L -0.7256 Soluble
12 S L -0.2688 Soluble
13 V L -0.4876 Soluble
14 A L -0.8114 Soluble
15 P L -1.7072 Soluble
16 G L -2.6865 Soluble
17 K L -2.8554 Soluble
18 T L -1.9627 Soluble
19 A L 0.0000 Buried
20 R L -1.8450 Soluble
21 I L 0.0000 Buried
22 T L -0.7298 Soluble
23 C L 0.0000 Buried
24 G L -0.9367 Soluble
25 G L -1.4731 Soluble
26 D L -2.7837 Soluble
27 N L -3.1622 Soluble
28 I L 0.0000 Buried
29 G L -2.0584 Soluble
36 G L -1.7093 Soluble
37 K L -1.0865 Soluble
38 S L -0.2932 Soluble
39 V L 0.0000 Buried
40 H L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -0.3606 Soluble
44 Q L -1.0924 Soluble
45 K L -1.6771 Soluble
46 P L -1.4126 Soluble
47 G L -1.4848 Soluble
48 Q L -1.7645 Soluble
49 A L -0.9857 Soluble
50 P L 0.0000 Buried
51 V L 0.5781 Aggregation-prone
52 L L 0.0000 Buried
53 V L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.4719 Soluble
56 Y L -0.1748 Soluble
57 D L -0.8181 Soluble
65 S L -1.1468 Soluble
66 D L -1.7638 Soluble
67 R L -1.8455 Soluble
68 P L -0.8453 Soluble
69 S L -0.7190 Soluble
70 G L -0.8026 Soluble
71 I L -0.6429 Soluble
72 P L -1.2477 Soluble
74 E L -2.2047 Soluble
75 R L -1.7173 Soluble
76 F L 0.0000 Buried
77 S L -1.3637 Soluble
78 G L -0.9461 Soluble
79 S L -0.6682 Soluble
80 N L -1.0878 Soluble
83 S L -1.4097 Soluble
84 G L -2.1757 Soluble
85 N L -2.8344 Soluble
86 T L -1.5430 Soluble
87 A L 0.0000 Buried
88 T L -0.7623 Soluble
89 L L 0.0000 Buried
90 T L -0.8994 Soluble
91 I L 0.0000 Buried
92 S L -2.4593 Soluble
93 R L -3.2835 Soluble
94 V L 0.0000 Buried
95 E L -2.6603 Soluble
96 A L -1.2893 Soluble
97 G L -1.1201 Soluble
98 D L 0.0000 Buried
99 E L 0.0000 Buried
100 A L 0.0000 Buried
101 D L -1.5985 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 V L 0.0000 Buried
107 L L -0.7373 Soluble
108 D L 0.0000 Buried
109 R L -2.9524 Soluble
110 R L -2.9333 Soluble
113 S L -2.0095 Soluble
114 D L -1.9568 Soluble
115 H L -1.1850 Soluble
116 W L 0.0000 Buried
117 L L 0.3804 Aggregation-prone
118 F L 0.2379 Aggregation-prone
119 G L 0.0000 Buried
120 G L -0.7764 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -2.1142 Soluble
124 L L 0.0000 Buried
125 T L -0.2844 Soluble
126 V L 0.0000 Buried
127 L L 0.5999 Aggregation-prone
128 G L -0.2715 Soluble
129 Q L 0.0000 Buried
130 P L -1.1181 Soluble
131 K L -2.2814 Soluble
132 A L -1.3866 Soluble
133 A L -0.8476 Soluble
134 P L -0.4815 Soluble
135 S L -0.3098 Soluble
136 V L -0.2991 Soluble
137 T L -0.2520 Soluble
138 L L 0.0000 Buried
139 F L 0.0000 Buried
140 P L 0.0000 Buried
141 P L 0.0000 Buried
142 S L -0.9210 Soluble
143 S L -1.3037 Soluble
144 E L -1.6245 Soluble
145 E L 0.0000 Buried
146 L L -1.9409 Soluble
147 Q L -2.1425 Soluble
148 A L -1.8123 Soluble
149 N L -2.7269 Soluble
150 K L -2.8568 Soluble
151 A L 0.0000 Buried
152 T L 0.0000 Buried
153 L L 0.0000 Buried
154 V L 0.0000 Buried
155 C L 0.0000 Buried
156 L L 0.0000 Buried
157 I L 0.0000 Buried
158 S L -0.6956 Soluble
159 D L -1.6352 Soluble
160 F L 0.0000 Buried
161 Y L 0.0000 Buried
162 P L -0.9058 Soluble
163 G L -0.7487 Soluble
164 A L -0.1937 Soluble
165 V L -0.0283 Soluble
166 T L -0.0613 Soluble
167 V L 0.1841 Aggregation-prone
168 A L -0.3415 Soluble
169 W L 0.0000 Buried
170 K L -0.9665 Soluble
171 A L 0.0000 Buried
172 D L -1.2190 Soluble
173 S L -0.8165 Soluble
174 S L -0.7006 Soluble
175 P L -0.8369 Soluble
176 V L -0.8170 Soluble
177 K L -1.5920 Soluble
178 A L -0.7842 Soluble
179 G L -0.5570 Soluble
180 V L -0.0820 Soluble
181 E L 0.0074 Aggregation-prone
182 T L 0.1359 Aggregation-prone
183 T L 0.0552 Aggregation-prone
184 T L -0.1091 Soluble
185 P L -0.2675 Soluble
186 S L -0.5946 Soluble
187 K L -1.3024 Soluble
188 Q L -1.2674 Soluble
189 S L -1.1684 Soluble
190 N L -1.6669 Soluble
191 N L -1.4449 Soluble
192 K L -1.6656 Soluble
193 Y L -0.8927 Soluble
194 A L 0.0000 Buried
195 A L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 Y L 0.0000 Buried
199 L L 0.0000 Buried
200 S L -0.6213 Soluble
201 L L 0.0000 Buried
202 T L -1.9680 Soluble
203 P L -2.5857 Soluble
204 E L -3.2175 Soluble
205 Q L -2.5174 Soluble
206 W L 0.0000 Buried
207 K L -3.1308 Soluble
208 S L -2.4126 Soluble
209 H L -2.3587 Soluble
210 R L -2.5967 Soluble
211 S L -1.5192 Soluble
212 Y L 0.0000 Buried
213 S L 0.0000 Buried
214 C L 0.0000 Buried
215 Q L -0.7214 Soluble
216 V L 0.0000 Buried
217 T L -0.5935 Soluble
218 H L 0.0000 Buried
219 E L -2.2792 Soluble
220 G L -1.4450 Soluble
221 S L -0.8974 Soluble
222 T L -0.6261 Soluble
223 V L -0.3602 Soluble
224 E L -1.5221 Soluble
225 K L -1.1666 Soluble
226 T L -0.8495 Soluble
227 V L 0.0000 Buried
228 A L -1.1941 Soluble
229 P L -1.2269 Soluble
230 T L -0.8952 Soluble
231 E L -1.4216 Soluble
232 C L -0.1429 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6769 1.6436 CSV PDB
4.5 -0.7128 1.6127 CSV PDB
5.0 -0.755 1.5741 CSV PDB
5.5 -0.7947 1.5336 CSV PDB
6.0 -0.8226 1.496 CSV PDB
6.5 -0.8313 1.4772 CSV PDB
7.0 -0.8201 1.4772 CSV PDB
7.5 -0.7955 1.4771 CSV PDB
8.0 -0.7638 1.4767 CSV PDB
8.5 -0.7272 1.5091 CSV PDB
9.0 -0.6861 1.6706 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH