Gremubamab / 6cyf

A4DAB136_6cyf_D_C | Source PDB: 6cyf | Chains: D, C | Done
98.7
Yes
10 Å
Yes
Avg A4D Score
-0.6604
Max Score
1.8358
Min Score
-3.3134
Total Score
-289.26
Score CDR
-0.1462
Score non-CDR
-0.7282

Calculation Parameters

Chain Sequence(s) VQLVESGGGLVQPGGSLRLSCAASGFTFSSYAMDWVRQAPGKGLEWVSAITMSGITAYYTDDVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKEEFLPGTHYFYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCAIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKLLIYSASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCLQDYNYPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) D (Heavy), C (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3134
Maximum Score 1.8358
Average Score -0.6604
Total Score -289.2625

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
2 V D 1.1147 Aggregation-prone
3 Q D -0.2225 Soluble
4 L D 0.0000 Buried
5 V D 0.5347 Aggregation-prone
6 E D 0.0000 Buried
7 S D -0.6023 Soluble
8 G D -1.0590 Soluble
9 G D -0.6136 Soluble
11 G D -0.1430 Soluble
12 L D 0.4457 Aggregation-prone
13 V D -0.3520 Soluble
14 Q D -1.4607 Soluble
15 P D -1.7244 Soluble
16 G D -1.4480 Soluble
17 G D -1.1389 Soluble
18 S D -1.4351 Soluble
19 L D -1.2851 Soluble
20 R D -2.3907 Soluble
21 L D 0.0000 Buried
22 S D -0.5377 Soluble
23 C D 0.0000 Buried
24 A D -0.2262 Soluble
25 A D 0.0000 Buried
26 S D -0.0760 Soluble
27 G D 0.0456 Aggregation-prone
28 F D 0.2536 Aggregation-prone
29 T D -0.0126 Soluble
30 F D 0.0000 Buried
35 S D -0.6763 Soluble
36 S D -0.2784 Soluble
37 Y D 0.0795 Aggregation-prone
38 A D -0.0016 Soluble
39 M D 0.0000 Buried
40 D D 0.0000 Buried
41 W D 0.0000 Buried
42 V D 0.0000 Buried
43 R D 0.0000 Buried
44 Q D -0.7646 Soluble
45 A D -1.1735 Soluble
46 P D -0.9383 Soluble
47 G D -1.5116 Soluble
48 K D -2.3615 Soluble
49 G D -1.6145 Soluble
50 L D 0.0000 Buried
51 E D -1.1454 Soluble
52 W D 0.0000 Buried
53 V D 0.0000 Buried
54 S D 0.0000 Buried
55 A D 0.0000 Buried
56 I D 0.0000 Buried
57 T D 0.3368 Aggregation-prone
58 M D 0.1312 Aggregation-prone
59 S D 0.2202 Aggregation-prone
62 G D 0.4594 Aggregation-prone
63 I D 1.8358 Aggregation-prone
64 T D 0.9423 Aggregation-prone
65 A D 0.6045 Aggregation-prone
66 Y D 0.0634 Aggregation-prone
67 Y D -0.6819 Soluble
68 T D 0.0000 Buried
69 D D -2.5215 Soluble
70 D D -2.1399 Soluble
71 V D 0.0000 Buried
72 K D -2.6103 Soluble
74 G D -1.8622 Soluble
75 R D -1.6269 Soluble
76 F D 0.0000 Buried
77 T D -0.9944 Soluble
78 I D 0.0000 Buried
79 S D -0.1686 Soluble
80 R D -0.7704 Soluble
81 D D -1.6987 Soluble
82 N D -1.7267 Soluble
83 S D -1.6082 Soluble
84 K D -2.4071 Soluble
85 N D -1.7312 Soluble
86 T D -1.0593 Soluble
87 L D 0.0000 Buried
88 Y D -0.6625 Soluble
89 L D 0.0000 Buried
90 Q D -1.6901 Soluble
91 M D 0.0000 Buried
92 N D -2.1279 Soluble
93 S D -1.4975 Soluble
94 L D 0.0000 Buried
95 R D -2.0479 Soluble
96 A D -1.5690 Soluble
97 E D -2.1519 Soluble
98 D D 0.0000 Buried
99 T D -0.6362 Soluble
100 A D 0.0000 Buried
101 V D 0.1997 Aggregation-prone
102 Y D 0.0000 Buried
103 Y D 0.0000 Buried
104 C D 0.0000 Buried
105 A D 0.0000 Buried
106 K D 0.0000 Buried
107 E D 0.0000 Buried
108 E D -0.0062 Soluble
109 F D 0.3530 Aggregation-prone
110 L D 0.3878 Aggregation-prone
111 P D -0.3048 Soluble
111A G D -0.5645 Soluble
111B T D -0.3543 Soluble
112B H D -0.5144 Soluble
112A Y D 0.4941 Aggregation-prone
112 F D 0.8673 Aggregation-prone
113 Y D 0.4727 Aggregation-prone
114 G D 0.0000 Buried
115 M D 0.0000 Buried
116 D D -0.7181 Soluble
117 V D -0.1038 Soluble
118 W D -0.4379 Soluble
119 G D 0.0000 Buried
120 Q D -1.4268 Soluble
121 G D 0.0000 Buried
122 T D -0.2095 Soluble
123 T D -0.0685 Soluble
124 V D 0.0000 Buried
125 T D -0.3678 Soluble
126 V D 0.0000 Buried
127 S D -0.8501 Soluble
128 S D -0.7021 Soluble
129 A D -0.4711 Soluble
130 S D -0.5718 Soluble
131 T D -0.8321 Soluble
132 K D -1.3466 Soluble
133 G D -1.4159 Soluble
134 P D -0.6364 Soluble
135 S D -0.4164 Soluble
136 V D 0.0000 Buried
137 F D 0.0000 Buried
138 P D -1.1168 Soluble
139 L D 0.0000 Buried
140 A D -1.0594 Soluble
141 P D 0.0000 Buried
142 S D -0.8298 Soluble
143 S D -1.2520 Soluble
144 K D -1.9690 Soluble
145 S D -1.3329 Soluble
146 G D -0.7512 Soluble
147 G D -0.8614 Soluble
148 T D -0.6892 Soluble
149 A D 0.0000 Buried
150 A D 0.0000 Buried
151 L D 0.0000 Buried
152 G D 0.0000 Buried
153 C D 0.0000 Buried
154 L D 0.0000 Buried
155 V D 0.0000 Buried
156 K D 0.0000 Buried
157 D D -0.4672 Soluble
158 Y D 0.0000 Buried
159 F D 0.0000 Buried
160 P D -0.8132 Soluble
161 E D -1.2356 Soluble
162 P D -1.1806 Soluble
163 V D -0.8787 Soluble
164 T D -0.6326 Soluble
165 V D -0.3174 Soluble
166 S D -0.3587 Soluble
167 W D 0.0000 Buried
168 N D -0.6671 Soluble
169 S D -0.6011 Soluble
170 G D -0.5703 Soluble
171 A D -0.2406 Soluble
172 L D -0.0147 Soluble
173 T D -0.1881 Soluble
174 S D -0.1922 Soluble
175 G D -0.2290 Soluble
176 V D 0.1283 Aggregation-prone
177 H D -0.2955 Soluble
178 T D 0.0193 Aggregation-prone
179 F D 0.0000 Buried
180 P D -0.3973 Soluble
181 A D 0.0057 Aggregation-prone
182 V D 0.2335 Aggregation-prone
183 L D 0.9265 Aggregation-prone
184 Q D 0.1134 Aggregation-prone
185 S D -0.0895 Soluble
186 S D -0.2418 Soluble
187 G D -0.0171 Soluble
188 L D -0.1152 Soluble
189 Y D 0.0000 Buried
190 S D 0.0000 Buried
191 L D 0.0000 Buried
192 S D 0.0000 Buried
193 S D 0.0000 Buried
194 V D 0.0000 Buried
195 V D 0.0000 Buried
196 T D -0.1718 Soluble
197 V D 0.0000 Buried
198 P D -0.5119 Soluble
199 S D -0.7549 Soluble
200 S D -0.6811 Soluble
201 S D -0.6392 Soluble
202 L D -0.7944 Soluble
203 G D -0.9897 Soluble
204 T D -0.7073 Soluble
205 Q D -1.1852 Soluble
206 T D -1.1225 Soluble
207 Y D 0.0000 Buried
208 I D -1.1152 Soluble
209 C D 0.0000 Buried
210 N D -1.2903 Soluble
211 V D 0.0000 Buried
212 N D -1.8046 Soluble
213 H D 0.0000 Buried
214 K D -2.7992 Soluble
215 P D -1.7967 Soluble
216 S D -1.8876 Soluble
217 N D -2.5682 Soluble
218 T D -2.0447 Soluble
219 K D -2.5290 Soluble
220 V D -1.2381 Soluble
221 D D -2.0249 Soluble
222 K D -1.7756 Soluble
223 K D -2.4894 Soluble
224 V D 0.0000 Buried
225 E D -2.7557 Soluble
226 P D -1.6932 Soluble
227 K D -1.8000 Soluble
228 S D -0.5364 Soluble
229 C D 0.2306 Aggregation-prone
1 A C -0.7232 Soluble
2 I C 0.0000 Buried
3 Q C -1.8716 Soluble
4 M C 0.0000 Buried
5 T C -1.4765 Soluble
6 Q C 0.0000 Buried
7 S C -0.8145 Soluble
8 P C -0.4267 Soluble
9 S C -0.7326 Soluble
10 S C -0.5310 Soluble
11 L C -0.3361 Soluble
12 S C -0.7127 Soluble
13 A C 0.0000 Buried
14 S C -0.3863 Soluble
15 V C 0.5315 Aggregation-prone
16 G C -0.6152 Soluble
17 D C -1.5891 Soluble
18 R C -2.2504 Soluble
19 V C 0.0000 Buried
20 T C -0.6041 Soluble
21 I C 0.0000 Buried
22 T C -0.8631 Soluble
23 C C 0.0000 Buried
24 R C -2.8577 Soluble
25 A C 0.0000 Buried
26 S C -1.8354 Soluble
27 Q C -2.3080 Soluble
28 G C -2.1155 Soluble
29 I C 0.0000 Buried
36 R C -2.7573 Soluble
37 N C -2.2048 Soluble
38 D C 0.0000 Buried
39 L C 0.0000 Buried
40 G C 0.0000 Buried
41 W C 0.0000 Buried
42 Y C 0.0000 Buried
43 Q C 0.0000 Buried
44 Q C 0.0000 Buried
45 K C -1.7729 Soluble
46 P C -1.5520 Soluble
47 G C -1.6508 Soluble
48 K C -2.6103 Soluble
49 A C -1.7181 Soluble
50 P C 0.0000 Buried
51 K C -1.8646 Soluble
52 L C 0.0000 Buried
53 L C 0.0000 Buried
54 I C 0.0000 Buried
55 Y C -0.0310 Soluble
56 S C -0.5234 Soluble
57 A C 0.0000 Buried
65 S C -0.4464 Soluble
66 T C 0.0411 Aggregation-prone
67 L C 0.2324 Aggregation-prone
68 Q C -0.3595 Soluble
69 S C -0.3726 Soluble
70 G C -0.5032 Soluble
71 V C -0.3697 Soluble
72 P C -0.3461 Soluble
74 S C -0.4245 Soluble
75 R C -0.7746 Soluble
76 F C 0.0000 Buried
77 S C -0.2918 Soluble
78 G C -0.2544 Soluble
79 S C -0.7588 Soluble
80 G C -1.5696 Soluble
83 S C -1.7982 Soluble
84 G C -2.2475 Soluble
85 T C -2.3655 Soluble
86 D C -2.6347 Soluble
87 F C 0.0000 Buried
88 T C -0.7494 Soluble
89 L C 0.0000 Buried
90 T C -0.5738 Soluble
91 I C 0.0000 Buried
92 S C -1.2901 Soluble
93 S C -1.0239 Soluble
94 L C 0.0000 Buried
95 Q C -0.7116 Soluble
96 P C -0.7605 Soluble
97 E C -1.8495 Soluble
98 D C 0.0000 Buried
99 F C -0.3451 Soluble
100 A C 0.0000 Buried
101 T C -0.5943 Soluble
102 Y C 0.0000 Buried
103 Y C 0.0000 Buried
104 C C 0.0000 Buried
105 L C 0.0000 Buried
106 Q C 0.0000 Buried
107 D C 0.0000 Buried
108 Y C -0.0200 Soluble
109 N C -0.2686 Soluble
114 Y C 0.0554 Aggregation-prone
115 P C -0.2823 Soluble
116 W C 0.0000 Buried
117 T C -0.5349 Soluble
118 F C 0.0000 Buried
119 G C 0.0000 Buried
120 Q C -1.6658 Soluble
121 G C 0.0000 Buried
122 T C 0.0000 Buried
123 K C -0.9782 Soluble
124 V C 0.0000 Buried
125 E C 0.0000 Buried
126 I C -0.1685 Soluble
127 K C -1.3737 Soluble
128 R C -1.1765 Soluble
129 T C -0.3335 Soluble
130 V C 0.0742 Aggregation-prone
131 A C -0.0237 Soluble
132 A C -0.2172 Soluble
133 P C 0.0000 Buried
134 S C -0.2080 Soluble
135 V C 0.0000 Buried
136 F C -0.0837 Soluble
137 I C 0.0000 Buried
138 F C 0.0000 Buried
139 P C -0.4447 Soluble
140 P C 0.0000 Buried
141 S C -1.6553 Soluble
142 D C -2.9605 Soluble
143 E C -2.7353 Soluble
144 Q C 0.0000 Buried
145 L C -2.1755 Soluble
146 K C -2.7309 Soluble
147 S C -1.7159 Soluble
148 G C -1.2849 Soluble
149 T C -0.8469 Soluble
150 A C 0.0000 Buried
151 S C 0.0000 Buried
152 V C 0.0000 Buried
153 V C 0.0000 Buried
154 C C 0.0000 Buried
155 L C 0.0000 Buried
156 L C 0.0000 Buried
157 N C -0.3752 Soluble
158 N C -0.7209 Soluble
159 F C 0.0000 Buried
160 Y C 0.0000 Buried
161 P C -1.4190 Soluble
162 R C -1.8287 Soluble
163 E C -2.7209 Soluble
164 A C -2.0631 Soluble
165 K C -2.1200 Soluble
166 V C -1.0874 Soluble
167 Q C -0.4443 Soluble
168 W C 0.0000 Buried
169 K C -0.4767 Soluble
170 V C 0.0000 Buried
171 D C -1.8138 Soluble
172 N C -1.3652 Soluble
173 A C -0.2829 Soluble
174 L C 0.7364 Aggregation-prone
175 Q C -0.3775 Soluble
176 S C -0.6567 Soluble
177 G C -1.4220 Soluble
178 N C -1.8338 Soluble
179 S C -1.8061 Soluble
180 Q C -2.0630 Soluble
181 E C -2.1497 Soluble
182 S C -0.8438 Soluble
183 V C -0.4550 Soluble
184 T C -0.9520 Soluble
185 E C -2.0705 Soluble
186 Q C -1.5675 Soluble
187 D C -1.7971 Soluble
188 S C -1.5840 Soluble
189 K C -2.1631 Soluble
190 D C -1.5615 Soluble
191 S C -1.5539 Soluble
192 T C 0.0000 Buried
193 Y C 0.0000 Buried
194 S C 0.0000 Buried
195 L C 0.0000 Buried
196 S C 0.0000 Buried
197 S C 0.0000 Buried
198 T C -0.9499 Soluble
199 L C 0.0000 Buried
200 T C -0.7325 Soluble
201 L C -0.7563 Soluble
202 S C -1.1329 Soluble
203 K C -1.9716 Soluble
204 A C -1.6969 Soluble
205 D C -2.2456 Soluble
206 Y C 0.0000 Buried
207 E C -3.1160 Soluble
208 K C -3.3134 Soluble
209 H C -2.8240 Soluble
210 K C -2.9196 Soluble
211 V C -1.3409 Soluble
212 Y C 0.0000 Buried
213 A C 0.0000 Buried
214 C C 0.0000 Buried
215 E C -0.7276 Soluble
216 V C 0.0000 Buried
217 T C -1.2320 Soluble
218 H C 0.0000 Buried
219 Q C -1.5639 Soluble
220 G C -0.5955 Soluble
221 L C -0.3623 Soluble
222 S C -0.5172 Soluble
223 S C -0.4546 Soluble
224 P C -0.5849 Soluble
225 V C -0.0599 Soluble
226 T C -0.4924 Soluble
227 K C -0.7486 Soluble
228 S C -0.5949 Soluble
229 F C -0.8921 Soluble
230 N C -1.7930 Soluble
231 R C -2.3820 Soluble
232 G C -2.1141 Soluble
233 E C -2.2142 Soluble
234 C C -0.6791 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6875 2.5558 CSV PDB
4.5 -0.7226 2.5558 CSV PDB
5.0 -0.7637 2.5558 CSV PDB
5.5 -0.802 2.5558 CSV PDB
6.0 -0.8264 2.5558 CSV PDB
6.5 -0.8282 2.5558 CSV PDB
7.0 -0.8078 2.5558 CSV PDB
7.5 -0.7727 2.5558 CSV PDB
8.0 -0.7297 2.5558 CSV PDB
8.5 -0.681 2.5558 CSV PDB
9.0 -0.6272 2.5558 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH