Otilimab / 5d7s

A4DAB198_5d7s_H_L | Source PDB: 5d7s | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6985
Max Score
1.8692
Min Score
-3.3384
Total Score
-295.45
Score CDR
-0.5793
Score non-CDR
-0.7119

Calculation Parameters

Chain Sequence(s) QVQLVESGGGLVQPGGSLRLSCAASGFTFSSYWMNWVRQAPGKGLEWVSGIENKYAGGATYYAASVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARGFGTDFWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKELTQPPSVSVAPGQTARISCSGDSIGKKYAYWYQQKPGQAPVLVIYKKRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCSAWGDKGMVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.3384
Maximum Score 1.8692
Average Score -0.6985
Total Score -295.4507

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.5047 Soluble
2 V H -0.8306 Soluble
3 Q H -1.2044 Soluble
4 L H 0.0000 Buried
5 V H 0.1729 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.2339 Soluble
8 G H -0.5616 Soluble
9 G H 0.2151 Aggregation-prone
11 G H 0.7021 Aggregation-prone
12 L H 1.4321 Aggregation-prone
13 V H -0.0231 Soluble
14 Q H -1.3517 Soluble
15 P H -1.6476 Soluble
16 G H -1.4789 Soluble
17 G H -1.0072 Soluble
18 S H -1.2618 Soluble
19 L H -0.7956 Soluble
20 R H -1.9905 Soluble
21 L H 0.0000 Buried
22 S H -0.5099 Soluble
23 C H 0.0000 Buried
24 A H -0.4246 Soluble
25 A H 0.0000 Buried
26 S H -1.0306 Soluble
27 G H -1.0544 Soluble
28 F H -0.3682 Soluble
29 T H -0.1064 Soluble
30 F H 0.0000 Buried
35 S H -0.8987 Soluble
36 S H -0.2253 Soluble
37 Y H 0.3100 Aggregation-prone
38 W H 0.0000 Buried
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.6218 Soluble
45 A H -1.0248 Soluble
46 P H -0.7227 Soluble
47 G H -1.4069 Soluble
48 K H -2.2955 Soluble
49 G H -1.5210 Soluble
50 L H 0.0000 Buried
51 E H -1.0066 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 G H 0.0000 Buried
56 I H 0.0000 Buried
57 E H -0.3225 Soluble
58 N H -0.9111 Soluble
59 K H -1.4724 Soluble
60 Y H 0.2453 Aggregation-prone
61 A H -0.2287 Soluble
62 G H -0.7599 Soluble
63 G H -0.7075 Soluble
64 A H -0.1934 Soluble
65 T H 0.2520 Aggregation-prone
66 Y H 0.3852 Aggregation-prone
67 Y H -0.3106 Soluble
68 A H -0.7057 Soluble
69 A H -0.7799 Soluble
70 S H -0.8904 Soluble
71 V H 0.0000 Buried
72 K H -1.8520 Soluble
74 G H -1.5005 Soluble
75 R H -1.3285 Soluble
76 F H 0.0000 Buried
77 T H -0.7804 Soluble
78 I H 0.0000 Buried
79 S H -0.4798 Soluble
80 R H -1.2479 Soluble
81 D H -1.6152 Soluble
82 N H -1.8882 Soluble
83 S H -1.5613 Soluble
84 K H -2.3742 Soluble
85 N H -1.6969 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.6070 Soluble
89 L H 0.0000 Buried
90 Q H -1.3553 Soluble
91 M H 0.0000 Buried
92 N H -1.4958 Soluble
93 S H -1.3072 Soluble
94 L H 0.0000 Buried
95 R H -2.6398 Soluble
96 A H -2.0013 Soluble
97 E H -2.4241 Soluble
98 D H 0.0000 Buried
99 T H -0.4376 Soluble
100 A H 0.0000 Buried
101 V H 0.7900 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H 0.0000 Buried
107 G H 0.0000 Buried
108 F H 1.6668 Aggregation-prone
114 G H 0.9175 Aggregation-prone
115 T H 0.0000 Buried
116 D H 1.0327 Aggregation-prone
117 F H 1.2584 Aggregation-prone
118 W H 0.6978 Aggregation-prone
119 G H 0.0000 Buried
120 Q H -1.0632 Soluble
121 G H 0.0000 Buried
122 T H 0.5252 Aggregation-prone
123 L H 1.5712 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.3180 Aggregation-prone
126 V H 0.0000 Buried
127 S H -0.7610 Soluble
128 S H -0.4640 Soluble
129 A H -0.1854 Soluble
130 S H -0.6267 Soluble
131 T H -0.8275 Soluble
132 K H -1.1652 Soluble
133 G H -1.4543 Soluble
134 P H -0.6256 Soluble
135 S H -0.2284 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.9695 Soluble
139 L H 0.0000 Buried
140 A H -1.1993 Soluble
141 P H -0.9668 Soluble
142 S H -0.9529 Soluble
143 S H -1.3175 Soluble
144 K H -1.9968 Soluble
145 S H -1.1596 Soluble
146 T H -0.9217 Soluble
147 S H -0.8915 Soluble
148 G H -1.0752 Soluble
149 G H -0.9201 Soluble
150 T H -0.8613 Soluble
151 A H 0.0000 Buried
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.2537 Soluble
160 Y H 0.0000 Buried
161 F H -0.4211 Soluble
162 P H -0.8049 Soluble
163 E H -0.9716 Soluble
164 P H -1.0678 Soluble
165 V H -0.9759 Soluble
166 T H -0.8192 Soluble
167 V H -0.4307 Soluble
168 S H -0.4139 Soluble
169 W H 0.0000 Buried
170 N H -0.7426 Soluble
171 S H -0.6491 Soluble
172 G H -0.4985 Soluble
173 A H -0.2479 Soluble
174 L H -0.0217 Soluble
175 T H -0.1993 Soluble
176 S H -0.2719 Soluble
177 G H -0.3390 Soluble
178 V H 0.1841 Aggregation-prone
179 H H -0.1222 Soluble
180 T H -0.0290 Soluble
181 F H 0.0000 Buried
182 P H -0.0692 Soluble
183 A H 0.0762 Aggregation-prone
184 V H 0.0000 Buried
185 L H 0.5093 Aggregation-prone
186 Q H 0.0263 Aggregation-prone
187 S H -0.5479 Soluble
188 S H -0.4276 Soluble
189 G H -0.2219 Soluble
190 L H 0.0220 Aggregation-prone
191 Y H 0.1840 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.1458 Aggregation-prone
197 V H 0.0000 Buried
198 T H -0.2281 Soluble
199 V H 0.0000 Buried
200 P H -0.5984 Soluble
201 S H -0.6015 Soluble
202 S H -0.5136 Soluble
203 S H -0.5379 Soluble
204 L H -0.6694 Soluble
205 G H -1.2349 Soluble
206 T H -0.6559 Soluble
207 Q H -1.1693 Soluble
208 T H -1.0863 Soluble
209 Y H 0.0000 Buried
210 I H -1.1168 Soluble
211 C H 0.0000 Buried
212 N H -1.4284 Soluble
213 V H 0.0000 Buried
214 N H -2.4842 Soluble
215 H H 0.0000 Buried
216 K H -2.9690 Soluble
217 P H -1.7511 Soluble
218 S H -1.9135 Soluble
219 N H -2.6766 Soluble
220 T H -2.2452 Soluble
221 K H -2.8367 Soluble
222 V H -1.5438 Soluble
223 D H -2.1426 Soluble
224 K H -1.7827 Soluble
225 K H -2.1450 Soluble
226 V H 0.0000 Buried
227 E H -2.7513 Soluble
228 P H -1.8544 Soluble
229 K H -2.2643 Soluble
3 E L -2.1229 Soluble
4 L L -1.2230 Soluble
5 T L -0.8017 Soluble
6 Q L 0.0000 Buried
7 P L -0.3644 Soluble
8 P L -0.5226 Soluble
9 S L -0.4375 Soluble
11 V L -0.4936 Soluble
12 S L -0.5608 Soluble
13 V L 0.0000 Buried
14 A L -0.7387 Soluble
15 P L -1.1743 Soluble
16 G L -1.4118 Soluble
17 Q L -1.6078 Soluble
18 T L -1.3282 Soluble
19 A L 0.0000 Buried
20 R L -1.9358 Soluble
21 I L 0.0000 Buried
22 S L -0.6177 Soluble
23 C L 0.0000 Buried
24 S L -1.3810 Soluble
25 G L -2.0898 Soluble
26 D L -2.9801 Soluble
27 S L -2.5906 Soluble
28 I L 0.0000 Buried
29 G L -2.2516 Soluble
36 K L -2.6863 Soluble
37 K L -1.5919 Soluble
38 Y L 0.0678 Aggregation-prone
39 A L 0.0000 Buried
40 Y L 0.5460 Aggregation-prone
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0605 Aggregation-prone
44 Q L 0.0000 Buried
45 K L -1.6411 Soluble
46 P L -1.3203 Soluble
47 G L -1.4669 Soluble
48 Q L -1.7613 Soluble
49 A L -0.8326 Soluble
50 P L 0.0000 Buried
51 V L 1.7743 Aggregation-prone
52 L L 1.7085 Aggregation-prone
53 V L 0.0000 Buried
54 I L 1.8692 Aggregation-prone
55 Y L -0.1406 Soluble
56 K L -2.2467 Soluble
65 K L -3.0457 Soluble
67 R L -3.3384 Soluble
68 P L -1.7601 Soluble
69 S L -1.0755 Soluble
70 G L -0.1111 Soluble
71 I L 1.3166 Aggregation-prone
72 P L -0.1146 Soluble
74 E L -1.3439 Soluble
75 R L -0.9203 Soluble
76 F L -0.4874 Soluble
77 S L -1.4000 Soluble
78 G L 0.0000 Buried
79 S L -1.2814 Soluble
80 N L -1.3682 Soluble
83 S L -1.4646 Soluble
84 G L -1.9716 Soluble
85 N L -2.5471 Soluble
86 T L -1.3258 Soluble
87 A L 0.0000 Buried
88 T L -0.6938 Soluble
89 L L 0.0000 Buried
90 T L -1.1095 Soluble
91 I L 0.0000 Buried
92 S L -1.4136 Soluble
93 G L -1.4393 Soluble
94 T L 0.0000 Buried
95 Q L -2.1374 Soluble
96 A L -1.6558 Soluble
97 E L -2.3841 Soluble
98 D L 0.0000 Buried
99 E L -1.6967 Soluble
100 A L 0.0000 Buried
101 D L -1.2402 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 S L 0.0000 Buried
106 A L 0.0000 Buried
107 W L -0.2768 Soluble
108 G L -1.8183 Soluble
109 D L -2.6468 Soluble
114 K L -2.3999 Soluble
115 G L -1.2080 Soluble
116 M L 0.0000 Buried
117 V L -0.6494 Soluble
118 F L -0.2418 Soluble
119 G L 0.0000 Buried
120 G L -0.9306 Soluble
121 G L -0.8835 Soluble
122 T L 0.0000 Buried
123 K L -0.9463 Soluble
124 L L 0.0000 Buried
125 T L 0.0000 Buried
126 V L 0.0000 Buried
127 L L -0.6603 Soluble
128 G L -1.0239 Soluble
129 Q L -1.1463 Soluble
130 P L -1.2449 Soluble
131 K L -2.0423 Soluble
132 A L -1.2634 Soluble
133 A L -0.7770 Soluble
134 P L -0.5242 Soluble
135 S L -0.4069 Soluble
136 V L 0.0000 Buried
137 T L -0.6335 Soluble
138 L L 0.0000 Buried
139 F L 0.0000 Buried
140 P L -0.0971 Soluble
141 P L 0.0000 Buried
142 S L -1.0812 Soluble
143 S L -1.6348 Soluble
144 E L -2.2996 Soluble
145 E L 0.0000 Buried
146 L L -1.8821 Soluble
147 Q L -2.2617 Soluble
148 A L -1.7748 Soluble
149 N L -2.4841 Soluble
150 K L -2.1898 Soluble
151 A L 0.0000 Buried
152 T L 0.0000 Buried
153 L L 0.0000 Buried
154 V L 0.0000 Buried
155 C L 0.0000 Buried
156 L L 0.0000 Buried
157 I L 0.0000 Buried
158 S L -0.8166 Soluble
159 D L -1.6281 Soluble
160 F L 0.0000 Buried
161 Y L 0.0000 Buried
162 P L 0.0000 Buried
163 G L 0.0000 Buried
164 A L -0.1250 Soluble
165 V L 0.0596 Aggregation-prone
166 T L 0.0280 Aggregation-prone
167 V L 0.0668 Aggregation-prone
168 A L -0.4465 Soluble
169 W L 0.0000 Buried
170 K L -1.0326 Soluble
171 A L -1.2054 Soluble
172 D L -1.4187 Soluble
173 S L -0.9268 Soluble
174 S L -0.7996 Soluble
175 P L -0.8747 Soluble
176 V L -0.8677 Soluble
177 K L -1.6822 Soluble
178 A L -0.8163 Soluble
179 G L -0.6419 Soluble
180 V L -0.4835 Soluble
181 E L -0.4363 Soluble
182 T L -0.0946 Soluble
183 T L -0.1778 Soluble
184 T L -0.6485 Soluble
185 P L -0.5713 Soluble
186 S L -1.2175 Soluble
187 K L -2.6095 Soluble
188 Q L -2.1409 Soluble
189 S L -1.6385 Soluble
190 N L -2.3323 Soluble
191 N L -2.7557 Soluble
192 K L -2.3707 Soluble
193 Y L -1.6109 Soluble
194 A L 0.0000 Buried
195 A L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 Y L 0.0000 Buried
199 L L 0.0000 Buried
200 S L -0.3960 Soluble
201 L L -0.9950 Soluble
202 T L -1.7371 Soluble
203 P L -2.3662 Soluble
204 E L -3.1474 Soluble
205 Q L -2.3550 Soluble
206 W L 0.0000 Buried
207 K L -3.1226 Soluble
208 S L -2.4337 Soluble
209 H L -2.4022 Soluble
210 R L -2.5373 Soluble
211 S L 0.0000 Buried
212 Y L 0.0000 Buried
213 S L 0.0000 Buried
214 C L 0.0000 Buried
215 Q L -0.9237 Soluble
216 V L 0.0000 Buried
217 T L -0.4717 Soluble
218 H L 0.0000 Buried
219 E L -1.2531 Soluble
220 G L -0.9369 Soluble
221 S L -0.6336 Soluble
222 T L -0.6409 Soluble
223 V L -0.7212 Soluble
224 E L -1.8007 Soluble
225 K L -1.4478 Soluble
226 T L -0.7769 Soluble
227 V L 0.0000 Buried
228 A L -0.9233 Soluble
229 P L -1.0404 Soluble
230 T L -0.7131 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6563 3.9522 CSV PDB
4.5 -0.6876 3.9286 CSV PDB
5.0 -0.7235 3.9028 CSV PDB
5.5 -0.7544 3.8761 CSV PDB
6.0 -0.7683 3.8498 CSV PDB
6.5 -0.7561 3.825 CSV PDB
7.0 -0.7191 3.8028 CSV PDB
7.5 -0.6665 3.7837 CSV PDB
8.0 -0.6059 3.7687 CSV PDB
8.5 -0.5407 3.7592 CSV PDB
9.0 -0.4722 3.7547 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH