Pamlectabart / 8qy9

A4DAB201_8qy9_H_L | Source PDB: 8qy9 | Chains: H, L | Done
98.24
Yes
10 Å
Yes
Avg A4D Score
-0.707
Max Score
1.5148
Min Score
-3.8951
Total Score
-300.47
Score CDR
-0.3856
Score non-CDR
-0.747

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSRYWMSWVRQAPGKGLVWVGEINPDSSTINYAPSLKDKFTISRDNAKNTLYLQMNSLRAEDTAVYYCASLYYDYGDAMDYWGQGTLVTVSSASTKGPSVFPLAPSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPAEIVMTQSPATLSVSPGERATLSCKASQSVDSNVAWYQQKPGQAPRALIYSASLRFSGIPARFSGSGGTEFTLTISSLQSEDFAVYYCQQYNNYPLTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8951
Maximum Score 1.5148
Average Score -0.707
Total Score -300.4734

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -2.0996 Soluble
2 V H -1.0578 Soluble
3 Q H -1.1675 Soluble
4 L H 0.0000 Buried
5 V H 1.0494 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.4299 Soluble
8 G H -0.9757 Soluble
9 G H -0.4663 Soluble
11 G H 0.0840 Aggregation-prone
12 L H 0.3004 Aggregation-prone
13 V H -0.5011 Soluble
14 Q H -1.5822 Soluble
15 P H -1.8308 Soluble
16 G H -1.5345 Soluble
17 G H -1.1863 Soluble
18 S H -1.4717 Soluble
19 L H -1.2346 Soluble
20 R H -2.2983 Soluble
21 L H 0.0000 Buried
22 S H -0.4244 Soluble
23 C H 0.0000 Buried
24 A H -0.2130 Soluble
25 A H 0.0000 Buried
26 S H -1.0236 Soluble
27 G H -1.2515 Soluble
28 F H -0.6773 Soluble
29 T H -0.6716 Soluble
30 F H 0.0000 Buried
35 S H -1.6990 Soluble
36 R H -1.9123 Soluble
37 Y H 0.0000 Buried
38 W H -0.0779 Soluble
39 M H 0.0000 Buried
40 S H 0.1456 Aggregation-prone
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0252 Aggregation-prone
44 Q H -0.3861 Soluble
45 A H -0.9144 Soluble
46 P H -0.9401 Soluble
47 G H -1.4874 Soluble
48 K H -1.9879 Soluble
49 G H -0.8970 Soluble
50 L H 0.0000 Buried
51 V H 0.6454 Aggregation-prone
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 G H 0.0000 Buried
55 E H 0.1102 Aggregation-prone
56 I H 0.0000 Buried
57 N H 0.0000 Buried
58 P H -1.4849 Soluble
59 D H -2.2219 Soluble
62 S H -1.0469 Soluble
63 S H -0.6970 Soluble
64 T H -0.1628 Soluble
65 I H 0.5003 Aggregation-prone
66 N H 0.4951 Aggregation-prone
67 Y H 0.1243 Aggregation-prone
68 A H 0.0000 Buried
69 P H -0.9555 Soluble
70 S H -1.1056 Soluble
71 L H -1.3845 Soluble
72 K H -2.6838 Soluble
74 D H -3.0305 Soluble
75 K H -2.3294 Soluble
76 F H 0.0000 Buried
77 T H -0.9787 Soluble
78 I H 0.0000 Buried
79 S H -0.2512 Soluble
80 R H 0.0000 Buried
81 D H -1.7965 Soluble
82 N H -2.0806 Soluble
83 A H -1.3893 Soluble
84 K H -2.3289 Soluble
85 N H -1.8146 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.5463 Soluble
89 L H 0.0000 Buried
90 Q H -1.7939 Soluble
91 M H 0.0000 Buried
92 N H -2.4917 Soluble
93 S H -2.0049 Soluble
94 L H 0.0000 Buried
95 R H -2.7141 Soluble
96 A H -1.8674 Soluble
97 E H -2.3383 Soluble
98 D H 0.0000 Buried
99 T H -0.7162 Soluble
100 A H 0.0000 Buried
101 V H 0.2732 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 S H 0.0000 Buried
107 L H 0.7456 Aggregation-prone
108 Y H 0.6242 Aggregation-prone
109 Y H 1.2070 Aggregation-prone
110 D H 0.6389 Aggregation-prone
111 Y H 0.9833 Aggregation-prone
112 G H 0.0058 Aggregation-prone
113 D H -0.4823 Soluble
114 A H 0.0000 Buried
115 M H 0.2255 Aggregation-prone
116 D H 0.2165 Aggregation-prone
117 Y H 0.1529 Aggregation-prone
118 W H -0.1773 Soluble
119 G H 0.0000 Buried
120 Q H -1.1429 Soluble
121 G H 0.0000 Buried
122 T H 0.0000 Buried
123 L H 0.3015 Aggregation-prone
124 V H 0.0000 Buried
125 T H -0.3319 Soluble
126 V H 0.0000 Buried
127 S H -1.0657 Soluble
128 S H -0.8664 Soluble
129 A H -0.5405 Soluble
130 S H -0.6868 Soluble
131 T H -0.8622 Soluble
132 K H -1.8080 Soluble
133 G H -1.8576 Soluble
134 P H -0.9883 Soluble
135 S H -0.6812 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2237 Soluble
139 L H 0.0000 Buried
140 A H -0.7616 Soluble
141 P H -0.4770 Soluble
142 S H -0.1547 Soluble
143 G H -0.6124 Soluble
144 T H -0.5149 Soluble
145 A H 0.0000 Buried
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H -0.4171 Soluble
153 D H -0.7716 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.6394 Soluble
158 P H -0.8860 Soluble
159 V H 0.0000 Buried
160 T H -0.7369 Soluble
161 V H -0.6137 Soluble
162 S H -0.6385 Soluble
163 W H 0.0000 Buried
164 N H -0.8967 Soluble
165 S H -0.7846 Soluble
166 G H -0.6411 Soluble
167 A H -0.2694 Soluble
168 L H -0.0091 Soluble
169 T H -0.1649 Soluble
170 S H -0.1459 Soluble
171 G H -0.2797 Soluble
172 V H 0.1505 Aggregation-prone
173 H H -0.3961 Soluble
174 T H 0.0136 Aggregation-prone
175 F H 0.0000 Buried
176 P H -0.3697 Soluble
177 A H 0.2900 Aggregation-prone
178 V H 0.7752 Aggregation-prone
179 L H 1.5148 Aggregation-prone
180 Q H 0.4070 Aggregation-prone
181 S H -0.0163 Soluble
182 S H -0.1978 Soluble
183 G H 0.1098 Aggregation-prone
184 L H 0.1004 Aggregation-prone
185 Y H 0.5431 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1333 Soluble
193 V H 0.0000 Buried
194 P H -0.4823 Soluble
195 S H -0.5611 Soluble
196 S H -0.6549 Soluble
197 S H -0.7103 Soluble
198 L H -1.0222 Soluble
199 G H -1.1042 Soluble
200 T H -0.8763 Soluble
201 Q H -1.5774 Soluble
202 T H -1.3572 Soluble
203 Y H 0.0000 Buried
204 I H -1.3893 Soluble
205 C H 0.0000 Buried
206 N H -2.2048 Soluble
207 V H 0.0000 Buried
208 N H -2.8505 Soluble
209 H H 0.0000 Buried
210 K H -2.9267 Soluble
211 P H -1.7939 Soluble
212 S H -2.0690 Soluble
213 N H -2.8004 Soluble
214 T H -2.3404 Soluble
215 K H -3.0310 Soluble
216 V H -1.9837 Soluble
217 D H -2.8971 Soluble
218 K H -2.3242 Soluble
219 R H -2.7593 Soluble
220 V H 0.0000 Buried
221 E H -2.4810 Soluble
222 P H -1.0570 Soluble
223 A H -0.4409 Soluble
1 E L -1.5164 Soluble
2 I L 0.0000 Buried
3 V L 0.6564 Aggregation-prone
4 M L 0.0000 Buried
5 T L -0.5609 Soluble
6 Q L 0.0000 Buried
7 S L -0.5839 Soluble
8 P L -0.2562 Soluble
9 A L -0.1482 Soluble
10 T L -0.1777 Soluble
11 L L -0.0701 Soluble
12 S L -0.4368 Soluble
13 V L 0.0000 Buried
14 S L -1.4566 Soluble
15 P L -1.7739 Soluble
16 G L -1.9155 Soluble
17 E L -2.7283 Soluble
18 R L -2.8040 Soluble
19 A L 0.0000 Buried
20 T L -0.5677 Soluble
21 L L 0.0000 Buried
22 S L -1.1173 Soluble
23 C L 0.0000 Buried
24 K L -2.5295 Soluble
25 A L 0.0000 Buried
26 S L -0.9352 Soluble
27 Q L -1.6899 Soluble
28 S L -1.9196 Soluble
29 V L 0.0000 Buried
36 D L -2.6394 Soluble
37 S L -1.4985 Soluble
38 N L -1.2384 Soluble
39 V L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -0.7005 Soluble
44 Q L 0.0000 Buried
45 K L -1.6519 Soluble
46 P L -1.4639 Soluble
47 G L -1.5263 Soluble
48 Q L -2.0764 Soluble
49 A L -1.2555 Soluble
50 P L 0.0000 Buried
51 R L -1.0325 Soluble
52 A L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0570 Aggregation-prone
56 S L -0.4526 Soluble
57 A L 0.0000 Buried
65 S L -0.5402 Soluble
66 L L -0.3967 Soluble
67 R L -1.4997 Soluble
68 F L 0.0000 Buried
69 S L -0.7428 Soluble
70 G L -0.8137 Soluble
71 I L 0.0000 Buried
72 P L -0.5732 Soluble
74 A L -0.5532 Soluble
75 R L -0.7195 Soluble
76 F L 0.0000 Buried
77 S L -0.6090 Soluble
78 G L 0.0000 Buried
79 S L -0.7232 Soluble
80 G L -1.7016 Soluble
84 G L -2.3227 Soluble
85 T L -2.4538 Soluble
86 E L -3.1463 Soluble
87 F L 0.0000 Buried
88 T L -0.9770 Soluble
89 L L 0.0000 Buried
90 T L -0.6836 Soluble
91 I L 0.0000 Buried
92 S L -1.5097 Soluble
93 S L -1.7945 Soluble
94 L L 0.0000 Buried
95 Q L -1.8405 Soluble
96 S L -1.7229 Soluble
97 E L -2.2449 Soluble
98 D L 0.0000 Buried
99 F L -0.7520 Soluble
100 A L 0.0000 Buried
101 V L -0.2782 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L -0.1910 Soluble
108 N L -1.1376 Soluble
109 N L -1.0296 Soluble
114 Y L 0.4139 Aggregation-prone
115 P L 0.2172 Aggregation-prone
116 L L 0.3010 Aggregation-prone
117 T L 0.3659 Aggregation-prone
118 F L 0.0000 Buried
119 G L 0.0854 Aggregation-prone
120 A L -0.1251 Soluble
121 G L -0.4582 Soluble
122 T L 0.0000 Buried
123 K L -0.4978 Soluble
124 L L 0.0000 Buried
125 E L -0.8022 Soluble
126 L L -0.8153 Soluble
127 K L -1.0724 Soluble
128 R L -0.8046 Soluble
129 T L 0.0125 Aggregation-prone
130 V L 0.7327 Aggregation-prone
131 A L 0.1216 Aggregation-prone
132 A L -0.1090 Soluble
133 P L -0.2091 Soluble
134 S L -0.2092 Soluble
135 V L 0.0000 Buried
136 F L 0.0682 Aggregation-prone
137 I L 0.0929 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6675 Soluble
140 P L 0.0000 Buried
141 S L -1.6795 Soluble
142 D L -3.0225 Soluble
143 E L -3.2594 Soluble
144 Q L 0.0000 Buried
145 L L -2.3637 Soluble
146 K L -2.8367 Soluble
147 S L -1.7733 Soluble
148 G L -1.2778 Soluble
149 T L -1.0081 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -1.1315 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.1857 Soluble
162 R L -1.9354 Soluble
163 E L -2.7827 Soluble
164 A L -2.1132 Soluble
165 K L -2.3954 Soluble
166 V L -1.1861 Soluble
167 Q L -0.7975 Soluble
168 W L 0.0000 Buried
169 K L -0.8160 Soluble
170 V L 0.0000 Buried
171 D L -1.4755 Soluble
172 N L -1.4029 Soluble
173 A L -0.5019 Soluble
174 L L 0.1308 Aggregation-prone
175 Q L -0.4737 Soluble
176 S L -0.5724 Soluble
177 G L -0.8709 Soluble
178 N L -0.7871 Soluble
179 S L -0.9699 Soluble
180 Q L -1.0302 Soluble
181 E L -1.5652 Soluble
182 S L -0.5469 Soluble
183 V L -0.3580 Soluble
184 T L -0.8575 Soluble
185 E L -2.1754 Soluble
186 Q L -2.0574 Soluble
187 D L -2.4799 Soluble
188 S L -2.2248 Soluble
189 K L -2.6019 Soluble
190 D L -2.2752 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.5432 Soluble
199 L L 0.0000 Buried
200 T L -0.0761 Soluble
201 L L -0.6583 Soluble
202 S L -1.1681 Soluble
203 K L -2.2096 Soluble
204 A L -2.2247 Soluble
205 D L -2.8985 Soluble
206 Y L 0.0000 Buried
207 E L -3.8951 Soluble
208 K L -3.8511 Soluble
209 H L -3.2532 Soluble
210 K L -3.3249 Soluble
211 V L -1.8006 Soluble
212 Y L 0.0000 Buried
213 A L -0.8634 Soluble
214 C L 0.0000 Buried
215 E L -0.8814 Soluble
216 V L 0.0000 Buried
217 T L -1.1418 Soluble
218 H L 0.0000 Buried
219 Q L -1.5370 Soluble
220 G L -0.4191 Soluble
221 L L -0.2447 Soluble
222 S L -0.4697 Soluble
223 S L -0.4311 Soluble
224 P L -0.5158 Soluble
225 V L -0.0932 Soluble
226 T L -0.7108 Soluble
227 K L -1.1783 Soluble
228 S L -0.8721 Soluble
229 F L -1.1459 Soluble
230 N L -2.3089 Soluble
231 R L -2.9752 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6812 1.8506 CSV PDB
4.5 -0.7279 1.7677 CSV PDB
5.0 -0.7843 1.6714 CSV PDB
5.5 -0.8402 1.6425 CSV PDB
6.0 -0.884 1.6267 CSV PDB
6.5 -0.9058 1.6102 CSV PDB
7.0 -0.9044 1.5936 CSV PDB
7.5 -0.8871 1.577 CSV PDB
8.0 -0.8609 1.5604 CSV PDB
8.5 -0.8283 1.6057 CSV PDB
9.0 -0.7893 1.6837 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH