Calculation Parameters
| Chain Sequence(s) |
QVQLQESGPGLVKPSETLSLTCSVSGGSITNYFWTWIRQSPGKGLEWIGYIYYSGGTNYNPSLKSRVTISIDTSKNQFSLNMNSVTAADTAVYYCAGSYYYGVDVWGQGTTVTVSSASTKGPSVFPLAPCSRLGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVEIVLTQSPATLSLSPGERATLSCRASQSIKSFLAWYRQKPGQAPRLLIYDASNRPTGIPARFSGSGSGTDFTLTINSLESEDFAVYFCQQRNNWPFTFGPGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFN
input.pdb
|
| Selected Chain(s) | A (Heavy), B (Light) |
| Distance of Aggregation | 10 Å |
| FoldX Energy Minimization | Yes |
| pH Calculations | Yes |
| Downloads | Download all data (.tar.gz) |
Aggrescan4D Profile
Per-Residue A4D Scores
Download CSVResidues with A4D score > 0.0 are highlighted as aggregation-prone.
| Index | Residue | Chain | A4D Score | Classification |
|---|---|---|---|---|
| 1 | Q | A | -1.7542 | Soluble |
| 2 | V | A | 0.0000 | Buried |
| 3 | Q | A | -2.1166 | Soluble |
| 4 | L | A | 0.0000 | Buried |
| 5 | Q | A | -1.9305 | Soluble |
| 6 | E | A | 0.0000 | Buried |
| 7 | S | A | -0.6479 | Soluble |
| 8 | G | A | -0.4213 | Soluble |
| 9 | P | A | -0.1505 | Soluble |
| 11 | G | A | 0.1655 | Aggregation-prone |
| 12 | L | A | 0.5363 | Aggregation-prone |
| 13 | V | A | 0.0000 | Buried |
| 14 | K | A | -1.7274 | Soluble |
| 15 | P | A | -1.4373 | Soluble |
| 16 | S | A | -1.6409 | Soluble |
| 17 | E | A | -2.3701 | Soluble |
| 18 | T | A | -1.8030 | Soluble |
| 19 | L | A | 0.0000 | Buried |
| 20 | S | A | -0.7979 | Soluble |
| 21 | L | A | 0.0000 | Buried |
| 22 | T | A | -0.5175 | Soluble |
| 23 | C | A | 0.0000 | Buried |
| 24 | S | A | -1.2797 | Soluble |
| 25 | V | A | 0.0000 | Buried |
| 26 | S | A | -1.5589 | Soluble |
| 27 | G | A | -1.3716 | Soluble |
| 28 | G | A | -1.1893 | Soluble |
| 29 | S | A | -0.5452 | Soluble |
| 30 | I | A | 0.0000 | Buried |
| 35 | T | A | -0.1138 | Soluble |
| 36 | N | A | -0.4867 | Soluble |
| 37 | Y | A | 0.0000 | Buried |
| 38 | F | A | 1.0038 | Aggregation-prone |
| 39 | W | A | 0.0000 | Buried |
| 40 | T | A | 0.0000 | Buried |
| 41 | W | A | 0.0000 | Buried |
| 42 | I | A | 0.0000 | Buried |
| 43 | R | A | 0.0000 | Buried |
| 44 | Q | A | -0.6189 | Soluble |
| 45 | S | A | 0.0000 | Buried |
| 46 | P | A | -1.0293 | Soluble |
| 47 | G | A | -1.4417 | Soluble |
| 48 | K | A | -2.0841 | Soluble |
| 49 | G | A | -1.3042 | Soluble |
| 50 | L | A | 0.0000 | Buried |
| 51 | E | A | -0.6455 | Soluble |
| 52 | W | A | 0.0000 | Buried |
| 53 | I | A | 0.0000 | Buried |
| 54 | G | A | 0.0000 | Buried |
| 55 | Y | A | 0.0000 | Buried |
| 56 | I | A | 0.0000 | Buried |
| 57 | Y | A | 0.9059 | Aggregation-prone |
| 58 | Y | A | 0.9246 | Aggregation-prone |
| 59 | S | A | 0.2182 | Aggregation-prone |
| 63 | G | A | -0.3189 | Soluble |
| 64 | G | A | -0.0653 | Soluble |
| 65 | T | A | -0.3927 | Soluble |
| 66 | N | A | -0.8538 | Soluble |
| 67 | Y | A | -0.8592 | Soluble |
| 68 | N | A | 0.0000 | Buried |
| 69 | P | A | -1.1206 | Soluble |
| 70 | S | A | -1.0069 | Soluble |
| 71 | L | A | 0.0000 | Buried |
| 72 | K | A | -2.0815 | Soluble |
| 74 | S | A | -1.4479 | Soluble |
| 75 | R | A | -1.6168 | Soluble |
| 76 | V | A | 0.0000 | Buried |
| 77 | T | A | -0.9267 | Soluble |
| 78 | I | A | 0.0000 | Buried |
| 79 | S | A | -0.2272 | Soluble |
| 80 | I | A | -0.3011 | Soluble |
| 81 | D | A | -1.2168 | Soluble |
| 82 | T | A | -1.0410 | Soluble |
| 83 | S | A | -1.3923 | Soluble |
| 84 | K | A | -2.2823 | Soluble |
| 85 | N | A | -1.5619 | Soluble |
| 86 | Q | A | -1.4656 | Soluble |
| 87 | F | A | 0.0000 | Buried |
| 88 | S | A | -0.3632 | Soluble |
| 89 | L | A | 0.0000 | Buried |
| 90 | N | A | -1.0736 | Soluble |
| 91 | M | A | 0.0000 | Buried |
| 92 | N | A | -2.1760 | Soluble |
| 93 | S | A | -1.7516 | Soluble |
| 94 | V | A | 0.0000 | Buried |
| 95 | T | A | -0.7585 | Soluble |
| 96 | A | A | -0.2847 | Soluble |
| 97 | A | A | -0.2264 | Soluble |
| 98 | D | A | 0.0000 | Buried |
| 99 | T | A | 0.0879 | Aggregation-prone |
| 100 | A | A | 0.0000 | Buried |
| 101 | V | A | 0.0964 | Aggregation-prone |
| 102 | Y | A | 0.0000 | Buried |
| 103 | Y | A | 0.0000 | Buried |
| 104 | C | A | 0.0000 | Buried |
| 105 | A | A | 0.0000 | Buried |
| 106 | G | A | 0.0000 | Buried |
| 107 | S | A | 0.0000 | Buried |
| 108 | Y | A | 1.1203 | Aggregation-prone |
| 109 | Y | A | 1.6182 | Aggregation-prone |
| 113 | Y | A | 0.7457 | Aggregation-prone |
| 114 | G | A | 0.0000 | Buried |
| 115 | V | A | 0.0000 | Buried |
| 116 | D | A | 0.0677 | Aggregation-prone |
| 117 | V | A | -0.2997 | Soluble |
| 118 | W | A | -0.7970 | Soluble |
| 119 | G | A | 0.0000 | Buried |
| 120 | Q | A | -1.9120 | Soluble |
| 121 | G | A | -1.0233 | Soluble |
| 122 | T | A | -0.4945 | Soluble |
| 123 | T | A | -0.1463 | Soluble |
| 124 | V | A | 0.0000 | Buried |
| 125 | T | A | -0.1318 | Soluble |
| 126 | V | A | 0.0000 | Buried |
| 127 | S | A | -0.6684 | Soluble |
| 128 | S | A | -0.4238 | Soluble |
| 129 | A | A | -0.4470 | Soluble |
| 130 | S | A | -0.5530 | Soluble |
| 131 | T | A | -0.7637 | Soluble |
| 132 | K | A | -1.3187 | Soluble |
| 133 | G | A | -0.8820 | Soluble |
| 134 | P | A | -0.7748 | Soluble |
| 135 | S | A | -0.6625 | Soluble |
| 136 | V | A | 0.0000 | Buried |
| 137 | F | A | 0.0000 | Buried |
| 138 | P | A | -0.6038 | Soluble |
| 139 | L | A | 0.0000 | Buried |
| 140 | A | A | 0.5018 | Aggregation-prone |
| 141 | P | A | 0.1006 | Aggregation-prone |
| 142 | C | A | 0.3707 | Aggregation-prone |
| 143 | S | A | -0.7332 | Soluble |
| 144 | R | A | -1.6729 | Soluble |
| 145 | L | A | 0.7165 | Aggregation-prone |
| 146 | G | A | 0.0000 | Buried |
| 147 | C | A | 0.0000 | Buried |
| 148 | L | A | 0.0000 | Buried |
| 149 | V | A | 0.0000 | Buried |
| 150 | K | A | -0.2568 | Soluble |
| 151 | D | A | -0.6850 | Soluble |
| 152 | Y | A | 0.0000 | Buried |
| 153 | F | A | 0.0000 | Buried |
| 154 | P | A | 0.0000 | Buried |
| 155 | E | A | -1.3834 | Soluble |
| 156 | P | A | -1.1755 | Soluble |
| 157 | V | A | -0.8906 | Soluble |
| 158 | T | A | -0.6056 | Soluble |
| 159 | V | A | -0.5022 | Soluble |
| 160 | S | A | -0.5105 | Soluble |
| 161 | W | A | 0.0000 | Buried |
| 162 | N | A | -0.9088 | Soluble |
| 163 | S | A | -0.7216 | Soluble |
| 164 | G | A | -0.4379 | Soluble |
| 165 | A | A | -0.1434 | Soluble |
| 166 | L | A | 0.0626 | Aggregation-prone |
| 167 | T | A | -0.0809 | Soluble |
| 168 | S | A | -0.1075 | Soluble |
| 169 | G | A | -0.0213 | Soluble |
| 170 | V | A | 0.3293 | Aggregation-prone |
| 171 | H | A | -0.0890 | Soluble |
| 172 | T | A | -0.1090 | Soluble |
| 173 | F | A | 0.0000 | Buried |
| 174 | P | A | -0.3058 | Soluble |
| 175 | A | A | 0.2899 | Aggregation-prone |
| 176 | V | A | 1.0158 | Aggregation-prone |
| 177 | L | A | 0.9582 | Aggregation-prone |
| 178 | Q | A | -0.2353 | Soluble |
| 179 | S | A | -0.2230 | Soluble |
| 180 | S | A | -0.3027 | Soluble |
| 181 | G | A | -0.0910 | Soluble |
| 182 | L | A | -0.2091 | Soluble |
| 183 | Y | A | 0.0798 | Aggregation-prone |
| 184 | S | A | -0.0226 | Soluble |
| 185 | L | A | 0.0000 | Buried |
| 186 | S | A | -0.0657 | Soluble |
| 187 | S | A | 0.0000 | Buried |
| 188 | V | A | 0.0000 | Buried |
| 189 | V | A | 0.0000 | Buried |
| 190 | T | A | 0.0313 | Aggregation-prone |
| 191 | V | A | 0.3458 | Aggregation-prone |
| 192 | P | A | -0.0254 | Soluble |
| 193 | S | A | -0.4486 | Soluble |
| 194 | S | A | -0.2354 | Soluble |
| 195 | S | A | -0.0379 | Soluble |
| 196 | L | A | 0.7378 | Aggregation-prone |
| 197 | G | A | -0.3237 | Soluble |
| 198 | T | A | -0.6677 | Soluble |
| 199 | K | A | -1.3298 | Soluble |
| 200 | T | A | -0.8496 | Soluble |
| 201 | Y | A | 0.0000 | Buried |
| 202 | T | A | -1.4630 | Soluble |
| 203 | C | A | 0.0000 | Buried |
| 204 | N | A | -1.8592 | Soluble |
| 205 | V | A | 0.0000 | Buried |
| 206 | D | A | -2.2062 | Soluble |
| 207 | H | A | 0.0000 | Buried |
| 208 | K | A | -2.9107 | Soluble |
| 209 | P | A | -1.8023 | Soluble |
| 210 | S | A | -1.8917 | Soluble |
| 211 | N | A | -2.5625 | Soluble |
| 212 | T | A | -2.2970 | Soluble |
| 213 | K | A | -2.7531 | Soluble |
| 214 | V | A | -1.7143 | Soluble |
| 215 | D | A | -2.7900 | Soluble |
| 216 | K | A | -1.9109 | Soluble |
| 217 | R | A | -2.1070 | Soluble |
| 218 | V | A | 0.0384 | Aggregation-prone |
| 1 | E | B | -1.6606 | Soluble |
| 2 | I | B | 0.0000 | Buried |
| 3 | V | B | 0.7151 | Aggregation-prone |
| 4 | L | B | 0.0000 | Buried |
| 5 | T | B | -0.5817 | Soluble |
| 6 | Q | B | 0.0000 | Buried |
| 7 | S | B | -0.6164 | Soluble |
| 8 | P | B | -0.2551 | Soluble |
| 9 | A | B | -0.1738 | Soluble |
| 10 | T | B | -0.0864 | Soluble |
| 11 | L | B | 0.0178 | Aggregation-prone |
| 12 | S | B | -0.2639 | Soluble |
| 13 | L | B | -0.7505 | Soluble |
| 14 | S | B | -1.3827 | Soluble |
| 15 | P | B | -1.8246 | Soluble |
| 16 | G | B | -2.0202 | Soluble |
| 17 | E | B | -2.5778 | Soluble |
| 18 | R | B | -2.9244 | Soluble |
| 19 | A | B | 0.0000 | Buried |
| 20 | T | B | -0.6876 | Soluble |
| 21 | L | B | 0.0000 | Buried |
| 22 | S | B | -0.9888 | Soluble |
| 23 | C | B | 0.0000 | Buried |
| 24 | R | B | -2.5192 | Soluble |
| 25 | A | B | 0.0000 | Buried |
| 26 | S | B | -1.1155 | Soluble |
| 27 | Q | B | -1.9306 | Soluble |
| 28 | S | B | -1.8300 | Soluble |
| 29 | I | B | 0.0000 | Buried |
| 36 | K | B | -2.2123 | Soluble |
| 37 | S | B | -1.2328 | Soluble |
| 38 | F | B | -0.4969 | Soluble |
| 39 | L | B | 0.0000 | Buried |
| 40 | A | B | 0.0000 | Buried |
| 41 | W | B | 0.0000 | Buried |
| 42 | Y | B | 0.0000 | Buried |
| 43 | R | B | -1.0259 | Soluble |
| 44 | Q | B | 0.0000 | Buried |
| 45 | K | B | -1.6760 | Soluble |
| 46 | P | B | -1.1821 | Soluble |
| 47 | G | B | -1.4789 | Soluble |
| 48 | Q | B | -2.0841 | Soluble |
| 49 | A | B | -1.3462 | Soluble |
| 50 | P | B | 0.0000 | Buried |
| 51 | R | B | -1.3541 | Soluble |
| 52 | L | B | 0.0000 | Buried |
| 53 | L | B | 0.0000 | Buried |
| 54 | I | B | 0.0000 | Buried |
| 55 | Y | B | -0.4077 | Soluble |
| 56 | D | B | -0.7531 | Soluble |
| 57 | A | B | 0.0000 | Buried |
| 65 | S | B | -1.0916 | Soluble |
| 66 | N | B | -1.6732 | Soluble |
| 67 | R | B | -1.3755 | Soluble |
| 68 | P | B | -0.7292 | Soluble |
| 69 | T | B | -0.5415 | Soluble |
| 70 | G | B | -0.5265 | Soluble |
| 71 | I | B | -0.4138 | Soluble |
| 72 | P | B | -0.3035 | Soluble |
| 74 | A | B | -0.4490 | Soluble |
| 75 | R | B | -1.1717 | Soluble |
| 76 | F | B | 0.0000 | Buried |
| 77 | S | B | -0.8053 | Soluble |
| 78 | G | B | 0.0000 | Buried |
| 79 | S | B | -0.8625 | Soluble |
| 80 | G | B | -1.3163 | Soluble |
| 83 | S | B | -1.5924 | Soluble |
| 84 | G | B | -1.8222 | Soluble |
| 85 | T | B | -2.2343 | Soluble |
| 86 | D | B | -2.6281 | Soluble |
| 87 | F | B | 0.0000 | Buried |
| 88 | T | B | -0.8349 | Soluble |
| 89 | L | B | 0.0000 | Buried |
| 90 | T | B | -0.9186 | Soluble |
| 91 | I | B | 0.0000 | Buried |
| 92 | N | B | -2.6852 | Soluble |
| 93 | S | B | -2.4924 | Soluble |
| 94 | L | B | 0.0000 | Buried |
| 95 | E | B | -2.9894 | Soluble |
| 96 | S | B | -1.9723 | Soluble |
| 97 | E | B | -2.6066 | Soluble |
| 98 | D | B | 0.0000 | Buried |
| 99 | F | B | -0.7197 | Soluble |
| 100 | A | B | 0.0000 | Buried |
| 101 | V | B | -0.0464 | Soluble |
| 102 | Y | B | 0.0000 | Buried |
| 103 | F | B | 0.0000 | Buried |
| 104 | C | B | 0.0000 | Buried |
| 105 | Q | B | 0.0000 | Buried |
| 106 | Q | B | 0.0000 | Buried |
| 107 | R | B | -0.4417 | Soluble |
| 108 | N | B | -1.3047 | Soluble |
| 109 | N | B | -1.4150 | Soluble |
| 114 | W | B | -0.6967 | Soluble |
| 115 | P | B | 0.0000 | Buried |
| 116 | F | B | -0.3931 | Soluble |
| 117 | T | B | -0.1948 | Soluble |
| 118 | F | B | 0.0000 | Buried |
| 119 | G | B | 0.0000 | Buried |
| 120 | P | B | -0.4679 | Soluble |
| 121 | G | B | -0.2362 | Soluble |
| 122 | T | B | 0.0000 | Buried |
| 123 | K | B | -0.2506 | Soluble |
| 124 | V | B | 0.0000 | Buried |
| 125 | D | B | 0.0000 | Buried |
| 126 | I | B | -0.5594 | Soluble |
| 127 | K | B | -1.3043 | Soluble |
| 128 | R | B | -1.4626 | Soluble |
| 129 | T | B | -0.2385 | Soluble |
| 130 | V | B | 0.6570 | Aggregation-prone |
| 131 | A | B | 0.1683 | Aggregation-prone |
| 132 | A | B | -0.0672 | Soluble |
| 133 | P | B | 0.0000 | Buried |
| 134 | S | B | -0.1381 | Soluble |
| 135 | V | B | 0.0000 | Buried |
| 136 | F | B | 0.6690 | Aggregation-prone |
| 137 | I | B | 0.4475 | Aggregation-prone |
| 138 | F | B | 0.0000 | Buried |
| 139 | P | B | -0.4057 | Soluble |
| 140 | P | B | -0.5508 | Soluble |
| 141 | S | B | -1.4000 | Soluble |
| 142 | D | B | -2.6164 | Soluble |
| 143 | E | B | -2.3916 | Soluble |
| 144 | Q | B | 0.0000 | Buried |
| 145 | L | B | -1.9517 | Soluble |
| 146 | K | B | -2.7367 | Soluble |
| 147 | S | B | -1.7852 | Soluble |
| 148 | G | B | -1.7342 | Soluble |
| 149 | T | B | -1.4026 | Soluble |
| 150 | A | B | 0.0000 | Buried |
| 151 | S | B | 0.0000 | Buried |
| 152 | V | B | 0.0000 | Buried |
| 153 | V | B | 0.0000 | Buried |
| 154 | C | B | 0.0000 | Buried |
| 155 | L | B | 0.0000 | Buried |
| 156 | L | B | 0.0000 | Buried |
| 157 | N | B | -0.4239 | Soluble |
| 158 | N | B | -0.6543 | Soluble |
| 159 | F | B | 0.0000 | Buried |
| 160 | Y | B | -1.0460 | Soluble |
| 161 | P | B | -2.0995 | Soluble |
| 162 | R | B | -3.0978 | Soluble |
| 163 | E | B | -3.2377 | Soluble |
| 164 | A | B | -1.9857 | Soluble |
| 165 | K | B | -2.0671 | Soluble |
| 166 | V | B | 0.0000 | Buried |
| 167 | Q | B | -0.6505 | Soluble |
| 168 | W | B | 0.8612 | Aggregation-prone |
| 169 | S | B | -0.7999 | Soluble |
| 170 | Q | B | -1.3453 | Soluble |
| 171 | E | B | -1.9301 | Soluble |
| 172 | S | B | 0.0362 | Aggregation-prone |
| 173 | V | B | 1.1351 | Aggregation-prone |
| 174 | T | B | 0.0000 | Buried |
| 175 | E | B | -1.5573 | Soluble |
| 176 | Q | B | -2.1010 | Soluble |
| 177 | D | B | -2.1503 | Soluble |
| 178 | S | B | -2.1037 | Soluble |
| 179 | K | B | -2.7898 | Soluble |
| 180 | D | B | -2.7682 | Soluble |
| 181 | S | B | -1.9434 | Soluble |
| 182 | T | B | 0.0000 | Buried |
| 183 | Y | B | 0.0000 | Buried |
| 184 | S | B | 0.0000 | Buried |
| 185 | L | B | -0.2223 | Soluble |
| 186 | S | B | 0.0000 | Buried |
| 187 | S | B | 0.0000 | Buried |
| 188 | T | B | -0.3033 | Soluble |
| 189 | L | B | 0.0000 | Buried |
| 190 | T | B | -0.5861 | Soluble |
| 191 | L | B | -0.5506 | Soluble |
| 192 | S | B | -1.1451 | Soluble |
| 193 | K | B | -2.3742 | Soluble |
| 194 | A | B | -1.8276 | Soluble |
| 195 | D | B | -2.1565 | Soluble |
| 196 | Y | B | 0.0000 | Buried |
| 197 | E | B | -3.7006 | Soluble |
| 198 | K | B | -3.3227 | Soluble |
| 199 | H | B | -2.5511 | Soluble |
| 200 | K | B | -2.5394 | Soluble |
| 201 | V | B | 0.1534 | Aggregation-prone |
| 202 | Y | B | 0.0000 | Buried |
| 203 | A | B | 0.0292 | Aggregation-prone |
| 204 | C | B | 0.0000 | Buried |
| 205 | E | B | -1.1958 | Soluble |
| 206 | V | B | 0.0000 | Buried |
| 207 | T | B | -1.5659 | Soluble |
| 208 | H | B | 0.0000 | Buried |
| 209 | Q | B | -1.7483 | Soluble |
| 210 | G | B | -0.5320 | Soluble |
| 211 | L | B | -0.1672 | Soluble |
| 212 | S | B | -0.4312 | Soluble |
| 213 | S | B | -0.4085 | Soluble |
| 214 | P | B | -0.5605 | Soluble |
| 215 | V | B | -0.0674 | Soluble |
| 216 | T | B | -0.5232 | Soluble |
| 217 | K | B | -0.4086 | Soluble |
| 218 | S | B | -0.2118 | Soluble |
| 219 | F | B | -0.2683 | Soluble |
| 220 | N | B | -1.6106 | Soluble |
3D Structure — colored by A4D score
pH-Dependent Aggregation Profiles
A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.
| pH | Avg A4D Score | Max A4D Score | Downloads |
|---|---|---|---|
| 4.0 | -0.6538 | 2.758 | CSV PDB |
| 4.5 | -0.6906 | 2.7462 | CSV PDB |
| 5.0 | -0.7341 | 2.7351 | CSV PDB |
| 5.5 | -0.7742 | 2.733 | CSV PDB |
| 6.0 | -0.7994 | 2.7517 | CSV PDB |
| 6.5 | -0.802 | 2.7956 | CSV PDB |
| 7.0 | -0.7828 | 2.852 | CSV PDB |
| 7.5 | -0.7497 | 2.905 | CSV PDB |
| 8.0 | -0.7093 | 2.9511 | CSV PDB |
| 8.5 | -0.6642 | 2.9933 | CSV PDB |
| 9.0 | -0.6146 | 3.0337 | CSV PDB |