Gremubamab / 6cyf

A4DAB136_6cyf_U_T | Source PDB: 6cyf | Chains: U, T | Done
98.7
Yes
10 Å
Yes
Avg A4D Score
-0.6767
Max Score
1.8384
Min Score
-3.4328
Total Score
-295.74
Score CDR
-0.1687
Score non-CDR
-0.7439

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYAMDWVRQAPGKGLEWVSAITMSGITAYYTDDVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKEEFLPGTHYFYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCAIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKLLIYSASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCLQDYNYPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) U (Heavy), T (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.4328
Maximum Score 1.8384
Average Score -0.6767
Total Score -295.7371

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E U -1.9959 Soluble
2 V U -1.0425 Soluble
3 Q U -1.0015 Soluble
4 L U 0.0000 Buried
5 V U 1.0960 Aggregation-prone
6 E U 0.1730 Aggregation-prone
7 S U -0.5893 Soluble
8 G U -1.2169 Soluble
9 G U -0.7469 Soluble
11 G U -0.3005 Soluble
12 L U 0.1369 Aggregation-prone
13 V U -0.5012 Soluble
14 Q U -1.6176 Soluble
15 P U -1.9240 Soluble
16 G U -1.5152 Soluble
17 G U -1.1235 Soluble
18 S U -1.2162 Soluble
19 L U -1.0861 Soluble
20 R U -2.1586 Soluble
21 L U 0.0000 Buried
22 S U -0.4553 Soluble
23 C U 0.0000 Buried
24 A U -0.0379 Soluble
25 A U 0.0000 Buried
26 S U -0.7174 Soluble
27 G U -1.0780 Soluble
28 F U -0.4161 Soluble
29 T U -0.1716 Soluble
30 F U 0.0000 Buried
35 S U -0.6558 Soluble
36 S U -0.2204 Soluble
37 Y U 0.0917 Aggregation-prone
38 A U 0.0062 Aggregation-prone
39 M U 0.0000 Buried
40 D U 0.0000 Buried
41 W U 0.0000 Buried
42 V U 0.0000 Buried
43 R U 0.0000 Buried
44 Q U -0.8113 Soluble
45 A U -1.1944 Soluble
46 P U -0.9811 Soluble
47 G U -1.5108 Soluble
48 K U -2.3756 Soluble
49 G U -1.6325 Soluble
50 L U 0.0000 Buried
51 E U -1.1341 Soluble
52 W U 0.0000 Buried
53 V U 0.0000 Buried
54 S U 0.0000 Buried
55 A U 0.0000 Buried
56 I U 0.0000 Buried
57 T U 0.3401 Aggregation-prone
58 M U 0.1407 Aggregation-prone
59 S U 0.2286 Aggregation-prone
62 G U 0.4653 Aggregation-prone
63 I U 1.8384 Aggregation-prone
64 T U 0.9452 Aggregation-prone
65 A U 0.6044 Aggregation-prone
66 Y U 0.0565 Aggregation-prone
67 Y U -0.8246 Soluble
68 T U 0.0000 Buried
69 D U -2.9063 Soluble
70 D U -2.9468 Soluble
71 V U 0.0000 Buried
72 K U -2.8532 Soluble
74 G U -1.9135 Soluble
75 R U -1.5417 Soluble
76 F U 0.0000 Buried
77 T U -0.7780 Soluble
78 I U 0.0000 Buried
79 S U -0.0943 Soluble
80 R U -0.7617 Soluble
81 D U -1.6878 Soluble
82 N U -1.7083 Soluble
83 S U -1.6011 Soluble
84 K U -2.4012 Soluble
85 N U -1.7436 Soluble
86 T U -1.0173 Soluble
87 L U 0.0000 Buried
88 Y U -0.5786 Soluble
89 L U 0.0000 Buried
90 Q U -1.1528 Soluble
91 M U 0.0000 Buried
92 N U -1.5476 Soluble
93 S U -1.4475 Soluble
94 L U 0.0000 Buried
95 R U -2.9018 Soluble
96 A U -1.9995 Soluble
97 E U -2.5359 Soluble
98 D U 0.0000 Buried
99 T U -0.8557 Soluble
100 A U 0.0000 Buried
101 V U 0.1450 Aggregation-prone
102 Y U 0.0000 Buried
103 Y U 0.0000 Buried
104 C U 0.0000 Buried
105 A U 0.0000 Buried
106 K U 0.0000 Buried
107 E U 0.0000 Buried
108 E U -0.0166 Soluble
109 F U 0.3567 Aggregation-prone
110 L U 0.4092 Aggregation-prone
111 P U -0.2962 Soluble
111A G U -0.5588 Soluble
111B T U -0.3411 Soluble
112B H U -0.4942 Soluble
112A Y U 0.5253 Aggregation-prone
112 F U 0.9087 Aggregation-prone
113 Y U 0.5060 Aggregation-prone
114 G U 0.0000 Buried
115 M U 0.0000 Buried
116 D U -0.8559 Soluble
117 V U -0.4456 Soluble
118 W U 0.0000 Buried
119 G U 0.0000 Buried
120 Q U -1.3272 Soluble
121 G U -0.6254 Soluble
122 T U -0.2544 Soluble
123 T U -0.1394 Soluble
124 V U 0.0000 Buried
125 T U 0.0000 Buried
126 V U 0.0000 Buried
127 S U -1.0530 Soluble
128 S U -0.7953 Soluble
129 A U -0.5030 Soluble
130 S U -0.6242 Soluble
131 T U -0.7251 Soluble
132 K U -1.4370 Soluble
133 G U -1.5723 Soluble
134 P U -0.7759 Soluble
135 S U -0.3447 Soluble
136 V U 0.0000 Buried
137 F U 0.0000 Buried
138 P U -1.0820 Soluble
139 L U 0.0000 Buried
140 A U -1.0837 Soluble
141 P U 0.0000 Buried
142 S U -0.9505 Soluble
143 S U -1.0964 Soluble
144 K U -1.8491 Soluble
145 G U -0.8334 Soluble
146 T U -0.6849 Soluble
147 A U 0.0000 Buried
148 A U 0.0000 Buried
149 L U 0.0000 Buried
150 G U 0.0000 Buried
151 C U 0.0000 Buried
152 L U 0.0000 Buried
153 V U 0.0000 Buried
154 K U 0.0000 Buried
155 D U -0.5412 Soluble
156 Y U 0.0000 Buried
157 F U 0.0000 Buried
158 P U 0.0000 Buried
159 E U -0.7068 Soluble
160 P U -0.9846 Soluble
161 V U -0.9263 Soluble
162 T U -0.8249 Soluble
163 V U -0.4921 Soluble
164 S U -0.4519 Soluble
165 W U 0.0000 Buried
166 N U -0.6629 Soluble
167 S U -0.6022 Soluble
168 G U -0.5878 Soluble
169 A U -0.2580 Soluble
170 L U -0.0487 Soluble
171 T U -0.2060 Soluble
172 S U -0.2109 Soluble
173 G U -0.2522 Soluble
174 V U 0.1176 Aggregation-prone
175 H U -0.2557 Soluble
176 T U 0.0474 Aggregation-prone
177 F U 0.0000 Buried
178 P U -0.3059 Soluble
179 A U 0.2091 Aggregation-prone
180 V U 0.4515 Aggregation-prone
181 L U 1.1059 Aggregation-prone
182 Q U 0.2522 Aggregation-prone
183 S U -0.0367 Soluble
184 S U -0.1983 Soluble
185 G U 0.0212 Aggregation-prone
186 L U -0.0189 Soluble
187 Y U 0.3092 Aggregation-prone
188 S U 0.0000 Buried
189 L U 0.0000 Buried
190 S U 0.0000 Buried
191 S U 0.0000 Buried
192 V U 0.0000 Buried
193 V U 0.0000 Buried
194 T U -0.1648 Soluble
195 V U 0.0000 Buried
196 P U -0.4725 Soluble
197 S U -0.7219 Soluble
198 S U -0.5896 Soluble
199 S U -0.5949 Soluble
200 L U -0.7655 Soluble
201 G U -0.9686 Soluble
202 T U -0.7024 Soluble
203 Q U -1.1742 Soluble
204 T U -1.1160 Soluble
205 Y U 0.0000 Buried
206 I U -1.0595 Soluble
207 C U 0.0000 Buried
208 N U -1.4319 Soluble
209 V U 0.0000 Buried
210 N U -2.4914 Soluble
211 H U 0.0000 Buried
212 K U -2.9136 Soluble
213 P U -1.7369 Soluble
214 S U -1.9704 Soluble
215 N U -2.6741 Soluble
216 T U -2.2385 Soluble
217 K U -2.7927 Soluble
218 V U -1.3508 Soluble
219 D U -1.9981 Soluble
220 K U -1.6726 Soluble
221 K U -2.4381 Soluble
222 V U 0.0000 Buried
223 E U -2.7837 Soluble
224 P U -1.8667 Soluble
225 K U -1.6823 Soluble
226 S U -0.6450 Soluble
227 C U 0.1357 Aggregation-prone
1 A T -0.7268 Soluble
2 I T 0.0000 Buried
3 Q T -1.8804 Soluble
4 M T 0.0000 Buried
5 T T -1.4850 Soluble
6 Q T 0.0000 Buried
7 S T -0.8997 Soluble
8 P T -0.4936 Soluble
9 S T -0.8431 Soluble
10 S T -0.6742 Soluble
11 L T -0.4287 Soluble
12 S T -0.7918 Soluble
13 A T 0.0000 Buried
14 S T -0.4619 Soluble
15 V T 0.4970 Aggregation-prone
16 G T -0.6182 Soluble
17 D T -1.5740 Soluble
18 R T -2.2473 Soluble
19 V T 0.0000 Buried
20 T T -0.6041 Soluble
21 I T 0.0000 Buried
22 T T -0.8170 Soluble
23 C T 0.0000 Buried
24 R T -2.7735 Soluble
25 A T -2.1146 Soluble
26 S T -1.8093 Soluble
27 Q T -2.2900 Soluble
28 G T -2.0470 Soluble
29 I T 0.0000 Buried
36 R T -2.5824 Soluble
37 N T -1.8697 Soluble
38 D T 0.0000 Buried
39 L T 0.0000 Buried
40 G T 0.0000 Buried
41 W T 0.0000 Buried
42 Y T 0.0000 Buried
43 Q T 0.0000 Buried
44 Q T 0.0000 Buried
45 K T -1.7456 Soluble
46 P T -1.5567 Soluble
47 G T -1.6550 Soluble
48 K T -2.5301 Soluble
49 A T -1.7196 Soluble
50 P T 0.0000 Buried
51 K T -1.9426 Soluble
52 L T 0.0000 Buried
53 L T 0.0000 Buried
54 I T 0.0000 Buried
55 Y T 0.0451 Aggregation-prone
56 S T -0.4190 Soluble
57 A T 0.0000 Buried
65 S T -0.3916 Soluble
66 T T 0.0195 Aggregation-prone
67 L T 0.1971 Aggregation-prone
68 Q T -0.3762 Soluble
69 S T -0.3692 Soluble
70 G T -0.4905 Soluble
71 V T -0.3399 Soluble
72 P T -0.3320 Soluble
74 S T -0.4231 Soluble
75 R T -0.7717 Soluble
76 F T 0.0000 Buried
77 S T -0.2846 Soluble
78 G T -0.2401 Soluble
79 S T -0.6506 Soluble
80 G T -1.3880 Soluble
83 S T -1.6308 Soluble
84 G T -2.1283 Soluble
85 T T -2.2271 Soluble
86 D T -2.3997 Soluble
87 F T 0.0000 Buried
88 T T -0.6827 Soluble
89 L T 0.0000 Buried
90 T T -0.5643 Soluble
91 I T 0.0000 Buried
92 S T -1.2861 Soluble
93 S T -1.0285 Soluble
94 L T 0.0000 Buried
95 Q T -0.7099 Soluble
96 P T -0.8226 Soluble
97 E T -1.8519 Soluble
98 D T 0.0000 Buried
99 F T -0.4706 Soluble
100 A T 0.0000 Buried
101 T T -0.7768 Soluble
102 Y T 0.0000 Buried
103 Y T 0.0000 Buried
104 C T 0.0000 Buried
105 L T 0.0000 Buried
106 Q T 0.0000 Buried
107 D T 0.0000 Buried
108 Y T 0.0934 Aggregation-prone
109 N T -0.2036 Soluble
114 Y T 0.0882 Aggregation-prone
115 P T -0.2700 Soluble
116 W T 0.0000 Buried
117 T T -0.5280 Soluble
118 F T 0.0000 Buried
119 G T 0.0000 Buried
120 Q T -1.7488 Soluble
121 G T 0.0000 Buried
122 T T 0.0000 Buried
123 K T -1.3689 Soluble
124 V T 0.0000 Buried
125 E T 0.0000 Buried
126 I T -0.4797 Soluble
127 K T -1.4450 Soluble
128 R T -1.2034 Soluble
129 T T -0.2045 Soluble
130 V T 0.3589 Aggregation-prone
131 A T 0.1376 Aggregation-prone
132 A T -0.1267 Soluble
133 P T 0.0000 Buried
134 S T -0.1792 Soluble
135 V T 0.0000 Buried
136 F T 0.0536 Aggregation-prone
137 I T 0.1294 Aggregation-prone
138 F T 0.0000 Buried
139 P T -0.4873 Soluble
140 P T 0.0000 Buried
141 S T -1.6286 Soluble
142 D T -2.7316 Soluble
143 E T -2.6327 Soluble
144 Q T 0.0000 Buried
145 L T -2.0899 Soluble
146 K T -2.6774 Soluble
147 S T -1.6881 Soluble
148 G T -1.2694 Soluble
149 T T -0.8507 Soluble
150 A T 0.0000 Buried
151 S T 0.0000 Buried
152 V T 0.0000 Buried
153 V T 0.0000 Buried
154 C T 0.0000 Buried
155 L T 0.0000 Buried
156 L T 0.0000 Buried
157 N T -0.3618 Soluble
158 N T -0.7428 Soluble
159 F T 0.0000 Buried
160 Y T 0.0000 Buried
161 P T -1.5181 Soluble
162 R T -2.1227 Soluble
163 E T -2.8937 Soluble
164 A T -2.1497 Soluble
165 K T -2.2229 Soluble
166 V T -1.0530 Soluble
167 Q T -0.5108 Soluble
168 W T 0.0000 Buried
169 K T -0.5249 Soluble
170 V T 0.0000 Buried
171 D T -1.9459 Soluble
172 N T -1.4130 Soluble
173 A T -0.3192 Soluble
174 L T 0.6903 Aggregation-prone
175 Q T -0.4145 Soluble
176 S T -0.6572 Soluble
177 G T -1.3367 Soluble
178 N T -1.6931 Soluble
179 S T -1.6243 Soluble
180 Q T -1.7420 Soluble
181 E T -1.9325 Soluble
182 S T -0.6212 Soluble
183 V T -0.2168 Soluble
184 T T -0.8469 Soluble
185 E T -2.0481 Soluble
186 Q T -1.6169 Soluble
187 D T -1.7445 Soluble
188 S T -1.5915 Soluble
189 K T -2.1439 Soluble
190 D T -1.5060 Soluble
191 S T -1.5802 Soluble
192 T T 0.0000 Buried
193 Y T 0.0000 Buried
194 S T 0.0000 Buried
195 L T 0.0000 Buried
196 S T 0.0000 Buried
197 S T 0.0000 Buried
198 T T -0.8096 Soluble
199 L T 0.0000 Buried
200 T T -0.6755 Soluble
201 L T -0.7855 Soluble
202 S T -1.1818 Soluble
203 K T -2.0269 Soluble
204 A T -1.7824 Soluble
205 D T -2.4057 Soluble
206 Y T 0.0000 Buried
207 E T -3.2625 Soluble
208 K T -3.4328 Soluble
209 H T -2.9808 Soluble
210 K T -3.1561 Soluble
211 V T -1.4620 Soluble
212 Y T 0.0000 Buried
213 A T 0.0000 Buried
214 C T 0.0000 Buried
215 E T -0.5638 Soluble
216 V T 0.0000 Buried
217 T T -1.1600 Soluble
218 H T 0.0000 Buried
219 Q T -1.7105 Soluble
220 G T -0.4858 Soluble
221 L T -0.2887 Soluble
222 S T -0.4864 Soluble
223 S T -0.4174 Soluble
224 P T -0.4763 Soluble
225 V T 0.1438 Aggregation-prone
226 T T -0.3157 Soluble
227 K T -0.5494 Soluble
228 S T -0.4929 Soluble
229 F T -0.8779 Soluble
230 N T -1.9435 Soluble
231 R T -2.5166 Soluble
232 G T -2.0382 Soluble
233 E T -2.2078 Soluble
234 C T -0.5952 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7388 2.5624 CSV PDB
4.5 -0.7782 2.5624 CSV PDB
5.0 -0.8224 2.5624 CSV PDB
5.5 -0.8619 2.5624 CSV PDB
6.0 -0.8862 2.5624 CSV PDB
6.5 -0.8868 2.5624 CSV PDB
7.0 -0.8649 2.5624 CSV PDB
7.5 -0.8288 2.5624 CSV PDB
8.0 -0.7851 2.5624 CSV PDB
8.5 -0.7356 2.5624 CSV PDB
9.0 -0.6806 2.5624 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH