Volagidemab / 4ers

A4DAB298_4ers_H_L | Source PDB: 4ers | Chains: H, L | Done
99.15
Yes
10 Å
Yes
Avg A4D Score
-0.7617
Max Score
2.8032
Min Score
-3.9147
Total Score
-338.98
Score CDR
-0.3933
Score non-CDR
-0.8137

Calculation Parameters

Chain Sequence(s) QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAVMYYDGSNKDYVDSVKGRFTISRDNSKNTLYLQMNRLRAEDTAVYYCAREKDHYDILTGYNYYYGLDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKRLIYAASSLESGVPSRFSGSGSGTEFTLTISSVQPEDFVTYYCLQHNSNPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.9147
Maximum Score 2.8032
Average Score -0.7617
Total Score -338.9772

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.1946 Soluble
2 V H -0.3261 Soluble
3 Q H -0.8501 Soluble
4 L H 0.0000 Buried
5 V H 0.2020 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.6882 Soluble
8 G H -0.9859 Soluble
9 G H -0.6640 Soluble
11 G H -0.0217 Soluble
12 V H 0.5328 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.3778 Soluble
15 P H -2.1147 Soluble
16 G H -2.6440 Soluble
17 R H -3.0367 Soluble
18 S H -2.4666 Soluble
19 L H -1.6517 Soluble
20 R H -2.1574 Soluble
21 L H 0.0000 Buried
22 S H -0.6053 Soluble
23 C H 0.0000 Buried
24 A H -0.4013 Soluble
25 A H 0.0000 Buried
26 S H -0.7450 Soluble
27 G H -0.6965 Soluble
28 F H -0.2151 Soluble
29 T H -0.1362 Soluble
30 F H 0.0000 Buried
35 S H -0.2622 Soluble
36 S H -0.1710 Soluble
37 Y H -0.1696 Soluble
38 G H 0.0000 Buried
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8205 Soluble
45 A H -1.2813 Soluble
46 P H -0.9722 Soluble
47 G H -1.4719 Soluble
48 K H -2.2422 Soluble
49 G H -1.4110 Soluble
50 L H 0.0000 Buried
51 E H -0.8681 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 V H 0.0000 Buried
56 M H 0.0000 Buried
57 Y H -0.5838 Soluble
58 Y H 0.0143 Aggregation-prone
59 D H -1.7567 Soluble
62 G H -1.6031 Soluble
63 S H -1.6500 Soluble
64 N H -2.1105 Soluble
65 K H -1.7555 Soluble
66 D H -1.6464 Soluble
67 Y H -1.3976 Soluble
68 V H -1.4272 Soluble
69 D H -2.4348 Soluble
70 S H -1.7655 Soluble
71 V H 0.0000 Buried
72 K H -2.6093 Soluble
74 G H -1.6895 Soluble
75 R H -1.8608 Soluble
76 F H 0.0000 Buried
77 T H -1.0452 Soluble
78 I H 0.0000 Buried
79 S H -0.4164 Soluble
80 R H -0.7689 Soluble
81 D H -1.5445 Soluble
82 N H -1.4727 Soluble
83 S H -1.5963 Soluble
84 K H -2.3684 Soluble
85 N H -1.7648 Soluble
86 T H -1.0795 Soluble
87 L H 0.0000 Buried
88 Y H -0.6764 Soluble
89 L H 0.0000 Buried
90 Q H -1.3809 Soluble
91 M H 0.0000 Buried
92 N H -2.4926 Soluble
93 R H -3.2483 Soluble
94 L H 0.0000 Buried
95 R H -3.1313 Soluble
96 A H -1.7246 Soluble
97 E H -2.3311 Soluble
98 D H 0.0000 Buried
99 T H -0.8222 Soluble
100 A H 0.0000 Buried
101 V H -0.0401 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.3115 Soluble
107 E H 0.0000 Buried
108 K H -1.0893 Soluble
109 D H -1.1000 Soluble
110 H H -0.2683 Soluble
111 Y H 1.3070 Aggregation-prone
111A D H 1.4999 Aggregation-prone
111B I H 2.8032 Aggregation-prone
111C L H 2.6416 Aggregation-prone
111D T H 1.5547 Aggregation-prone
112D G H 1.5038 Aggregation-prone
112C Y H 1.6869 Aggregation-prone
112B N H 0.3794 Aggregation-prone
112A Y H 0.4045 Aggregation-prone
112 Y H 0.1825 Aggregation-prone
113 Y H 0.1394 Aggregation-prone
114 G H 0.0000 Buried
115 L H 0.0000 Buried
116 D H -0.3600 Soluble
117 V H -0.0655 Soluble
118 W H -0.2971 Soluble
119 G H 0.0000 Buried
120 Q H -1.4781 Soluble
121 G H -0.6757 Soluble
122 T H -0.3796 Soluble
123 T H -0.1242 Soluble
124 V H 0.0000 Buried
125 T H -0.3514 Soluble
126 V H 0.0000 Buried
127 S H -0.4139 Soluble
128 S H -0.3110 Soluble
129 A H -0.2687 Soluble
130 S H -0.4086 Soluble
131 T H -0.6895 Soluble
132 K H -1.0630 Soluble
133 G H -1.3470 Soluble
134 P H 0.0000 Buried
135 S H -0.3477 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H 0.0000 Buried
139 L H 0.0000 Buried
140 A H -1.2064 Soluble
141 P H 0.0000 Buried
142 S H -1.0353 Soluble
143 S H -1.6138 Soluble
144 K H -2.1177 Soluble
145 S H -1.2746 Soluble
146 T H -0.8935 Soluble
147 S H -0.7146 Soluble
148 G H -0.9550 Soluble
149 G H -0.9212 Soluble
150 T H -0.6219 Soluble
151 A H -0.3521 Soluble
152 A H 0.0000 Buried
153 L H 0.0000 Buried
154 G H 0.0000 Buried
155 C H 0.0000 Buried
156 L H 0.0000 Buried
157 V H 0.0000 Buried
158 K H 0.0000 Buried
159 D H -0.4428 Soluble
160 Y H 0.0000 Buried
161 F H 0.0000 Buried
162 P H -0.7673 Soluble
163 E H -1.0945 Soluble
164 P H -1.0689 Soluble
165 V H -0.9195 Soluble
166 T H -0.7411 Soluble
167 V H -0.2676 Soluble
168 S H -0.4370 Soluble
169 W H 0.0000 Buried
170 N H -0.6751 Soluble
171 S H -0.6380 Soluble
172 G H -0.4921 Soluble
173 A H -0.2234 Soluble
174 L H 0.0176 Aggregation-prone
175 T H -0.1598 Soluble
176 S H -0.1634 Soluble
177 G H -0.1787 Soluble
178 V H 0.1397 Aggregation-prone
179 H H -0.2862 Soluble
180 T H 0.0212 Aggregation-prone
181 F H 0.0000 Buried
182 P H -0.3401 Soluble
183 A H 0.0805 Aggregation-prone
184 V H 0.3916 Aggregation-prone
185 L H 0.9481 Aggregation-prone
186 Q H 0.1819 Aggregation-prone
187 S H -0.2543 Soluble
188 S H -0.2171 Soluble
189 G H -0.0090 Soluble
190 L H 0.0355 Aggregation-prone
191 Y H 0.2478 Aggregation-prone
192 S H 0.0000 Buried
193 L H 0.0000 Buried
194 S H 0.0000 Buried
195 S H 0.0000 Buried
196 V H 0.0000 Buried
197 V H 0.0000 Buried
198 T H -0.1292 Soluble
199 V H 0.0000 Buried
200 P H -0.5403 Soluble
201 S H -0.6266 Soluble
202 S H -0.7048 Soluble
203 S H -0.5886 Soluble
204 L H -0.7613 Soluble
205 G H -0.9803 Soluble
206 T H -0.7407 Soluble
207 Q H -1.2542 Soluble
208 T H -1.0907 Soluble
209 Y H 0.0000 Buried
210 I H -1.1107 Soluble
211 C H 0.0000 Buried
212 N H -1.4001 Soluble
213 V H 0.0000 Buried
214 N H -2.1613 Soluble
215 H H 0.0000 Buried
216 K H -2.8769 Soluble
217 P H -1.7207 Soluble
218 S H -1.8356 Soluble
219 N H -2.6139 Soluble
220 T H -2.1348 Soluble
221 K H -2.7251 Soluble
222 V H -1.3745 Soluble
223 D H -2.3043 Soluble
224 K H -1.7649 Soluble
225 K H -2.1789 Soluble
226 V H 0.0000 Buried
227 E H -2.7450 Soluble
228 P H -2.1693 Soluble
229 K H -2.5347 Soluble
230 S H -1.1027 Soluble
231 C H -0.4550 Soluble
1 D L -2.5617 Soluble
2 I L 0.0000 Buried
3 Q L -2.3181 Soluble
4 M L 0.0000 Buried
5 T L -1.5024 Soluble
6 Q L -1.0268 Soluble
7 S L -0.6604 Soluble
8 P L -0.6294 Soluble
9 S L -0.8436 Soluble
10 S L -0.8957 Soluble
11 L L -0.6307 Soluble
12 S L -1.0074 Soluble
13 A L 0.0000 Buried
14 S L -0.7858 Soluble
15 V L 0.0135 Aggregation-prone
16 G L -0.8291 Soluble
17 D L -1.8686 Soluble
18 R L -2.3752 Soluble
19 V L 0.0000 Buried
20 T L -0.6456 Soluble
21 I L 0.0000 Buried
22 T L -0.9989 Soluble
23 C L 0.0000 Buried
24 R L -3.1295 Soluble
25 A L 0.0000 Buried
26 S L -2.2159 Soluble
27 Q L -2.7626 Soluble
28 G L -2.4264 Soluble
29 I L 0.0000 Buried
36 R L -3.1813 Soluble
37 N L -2.4552 Soluble
38 D L -1.3622 Soluble
39 L L 0.0000 Buried
40 G L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.3557 Soluble
45 K L -1.6993 Soluble
46 P L -1.4616 Soluble
47 G L -1.5590 Soluble
48 K L -2.2829 Soluble
49 A L -1.5227 Soluble
50 P L 0.0000 Buried
51 K L -1.4294 Soluble
52 R L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.3807 Aggregation-prone
56 A L -0.3102 Soluble
57 A L 0.0000 Buried
65 S L -0.4187 Soluble
66 S L 0.0666 Aggregation-prone
67 L L 0.3443 Aggregation-prone
68 E L -0.3120 Soluble
69 S L -0.4002 Soluble
70 G L -0.5041 Soluble
71 V L -0.1868 Soluble
72 P L -0.3107 Soluble
74 S L -0.3852 Soluble
75 R L -0.6867 Soluble
76 F L 0.0000 Buried
77 S L -0.2700 Soluble
78 G L -0.2360 Soluble
79 S L -0.9317 Soluble
80 G L -1.7222 Soluble
83 S L -2.2725 Soluble
84 G L -2.5593 Soluble
85 T L -2.6246 Soluble
86 E L -3.2600 Soluble
87 F L 0.0000 Buried
88 T L -0.9614 Soluble
89 L L 0.0000 Buried
90 T L -0.6151 Soluble
91 I L 0.0000 Buried
92 S L -1.3769 Soluble
93 S L -1.2155 Soluble
94 V L 0.0000 Buried
95 Q L -0.7233 Soluble
96 P L -0.7639 Soluble
97 E L -1.5714 Soluble
98 D L 0.0000 Buried
99 F L -0.6007 Soluble
100 V L 0.0000 Buried
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 L L 0.0000 Buried
106 Q L 0.0000 Buried
107 H L -0.7135 Soluble
108 N L -1.5579 Soluble
109 S L -1.5510 Soluble
114 N L -1.8612 Soluble
115 P L -1.8030 Soluble
116 L L 0.0000 Buried
117 T L -1.0409 Soluble
118 F L 0.0000 Buried
119 G L 0.0000 Buried
120 G L -1.2280 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.7954 Soluble
124 V L 0.0000 Buried
125 E L -1.0356 Soluble
126 I L -0.8355 Soluble
127 K L -1.6212 Soluble
128 R L -1.2845 Soluble
129 T L -0.2210 Soluble
130 V L 0.4194 Aggregation-prone
131 A L 0.0508 Aggregation-prone
132 A L -0.1323 Soluble
133 P L 0.0000 Buried
134 S L -0.1126 Soluble
135 V L 0.0000 Buried
136 F L 0.0167 Aggregation-prone
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L -1.0476 Soluble
140 P L -1.1647 Soluble
141 S L -1.8364 Soluble
142 D L -2.8823 Soluble
143 E L -2.2453 Soluble
144 Q L 0.0000 Buried
145 L L -1.8113 Soluble
146 K L -2.5477 Soluble
147 S L -1.5614 Soluble
148 G L -1.0987 Soluble
149 T L -0.7857 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L -0.3067 Soluble
158 N L -0.7172 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.3326 Soluble
162 R L -2.0672 Soluble
163 E L -2.7252 Soluble
164 A L -2.0671 Soluble
165 K L -2.3216 Soluble
166 V L -1.0448 Soluble
167 Q L -0.6620 Soluble
168 W L 0.0000 Buried
169 K L -0.6843 Soluble
170 V L 0.0000 Buried
171 D L -2.1761 Soluble
172 N L -1.6843 Soluble
173 A L -0.4491 Soluble
174 L L 0.5751 Aggregation-prone
175 Q L -0.5903 Soluble
176 S L -0.7905 Soluble
177 G L -1.3037 Soluble
178 N L -1.6129 Soluble
179 S L -1.5280 Soluble
180 Q L -1.5712 Soluble
181 E L -1.8469 Soluble
182 S L -0.6663 Soluble
183 V L -0.3229 Soluble
184 T L -0.9110 Soluble
185 E L -2.1190 Soluble
186 Q L -1.7698 Soluble
187 D L -2.0220 Soluble
188 S L -1.7791 Soluble
189 K L -2.3480 Soluble
190 D L -1.8656 Soluble
191 S L -1.8922 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7230 Soluble
199 L L 0.0000 Buried
200 T L -0.5247 Soluble
201 L L -0.5402 Soluble
202 S L -0.9511 Soluble
203 K L -1.9587 Soluble
204 A L -1.8051 Soluble
205 D L -2.4497 Soluble
206 Y L 0.0000 Buried
207 E L -3.9147 Soluble
208 K L -3.7749 Soluble
209 H L -3.4839 Soluble
210 K L -3.8238 Soluble
211 V L -2.2499 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.5838 Soluble
216 V L 0.0000 Buried
217 T L -1.2080 Soluble
218 H L 0.0000 Buried
219 Q L -1.7291 Soluble
220 G L -0.5123 Soluble
221 L L -0.3464 Soluble
222 S L -0.5056 Soluble
223 S L -0.4162 Soluble
224 P L -0.4533 Soluble
225 V L 0.1980 Aggregation-prone
226 T L -0.3688 Soluble
227 K L -0.9348 Soluble
228 S L -1.1428 Soluble
229 F L 0.0000 Buried
230 N L -2.5525 Soluble
231 R L -3.5952 Soluble
232 G L -2.3704 Soluble
233 E L -2.8751 Soluble
234 C L -1.6687 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.843 4.0604 CSV PDB
4.5 -0.8866 4.0096 CSV PDB
5.0 -0.9373 3.9588 CSV PDB
5.5 -0.9851 3.9135 CSV PDB
6.0 -1.018 3.8761 CSV PDB
6.5 -1.027 3.8414 CSV PDB
7.0 -1.0126 3.8012 CSV PDB
7.5 -0.9826 3.755 CSV PDB
8.0 -0.9439 3.7097 CSV PDB
8.5 -0.8985 3.674 CSV PDB
9.0 -0.8462 3.6525 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH