Amubarvimab / 7xnf

A4DAB014_7xnf_H_L | Source PDB: 7xnf | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.6964
Max Score
2.4496
Min Score
-3.6847
Total Score
-297.37
Score CDR
0.0013
Score non-CDR
-0.7766

Calculation Parameters

Chain Sequence(s) EVQLVESGGGLVQPGGSLRLSCAASGITVSSNYMNWVRQAPGKGLEWVSLIYSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYHCARDLVVYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6847
Maximum Score 2.4496
Average Score -0.6964
Total Score -297.3734

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.4823 Soluble
2 V H 0.1110 Aggregation-prone
3 Q H -0.8129 Soluble
4 L H 0.0000 Buried
5 V H 0.5000 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5100 Soluble
8 G H -0.9319 Soluble
9 G H -0.4318 Soluble
11 G H 0.1204 Aggregation-prone
12 L H 0.8330 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.5235 Soluble
15 P H -1.9200 Soluble
16 G H -1.7602 Soluble
17 G H -1.2652 Soluble
18 S H -1.3389 Soluble
19 L H -1.0734 Soluble
20 R H -2.0407 Soluble
21 L H 0.0000 Buried
22 S H -0.5595 Soluble
23 C H 0.0000 Buried
24 A H -0.3273 Soluble
25 A H -0.4483 Soluble
26 S H -0.5202 Soluble
27 G H -0.6739 Soluble
28 I H 0.0000 Buried
29 T H -0.3715 Soluble
30 V H 0.0000 Buried
35 S H -0.7982 Soluble
36 S H -0.4878 Soluble
37 N H -0.0920 Soluble
38 Y H 0.2416 Aggregation-prone
39 M H 0.0000 Buried
40 N H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.8954 Soluble
45 A H -1.3098 Soluble
46 P H -0.9718 Soluble
47 G H -1.4621 Soluble
48 K H -2.4272 Soluble
49 G H -1.6272 Soluble
50 L H 0.0000 Buried
51 E H -1.2048 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 L H 0.0000 Buried
56 I H 0.0000 Buried
57 Y H 0.0390 Aggregation-prone
58 S H -0.3586 Soluble
59 G H -0.6350 Soluble
63 G H -0.5749 Soluble
64 S H -0.2598 Soluble
65 T H 0.1004 Aggregation-prone
66 Y H 0.0981 Aggregation-prone
67 Y H -0.4963 Soluble
68 A H -1.2966 Soluble
69 D H -2.4273 Soluble
70 S H -1.8111 Soluble
71 V H 0.0000 Buried
72 K H -2.5382 Soluble
74 G H -1.8348 Soluble
75 R H -1.7864 Soluble
76 F H 0.0000 Buried
77 T H -0.9656 Soluble
78 I H 0.0000 Buried
79 S H -0.6503 Soluble
80 R H -1.4237 Soluble
81 D H -2.1167 Soluble
82 N H -2.4247 Soluble
83 S H -1.9645 Soluble
84 K H -2.5946 Soluble
85 N H -1.9533 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H 0.0000 Buried
89 L H 0.0000 Buried
90 Q H -1.6334 Soluble
91 M H 0.0000 Buried
92 N H -2.0850 Soluble
93 S H -1.6581 Soluble
94 L H 0.0000 Buried
95 R H -2.9795 Soluble
96 A H -2.0188 Soluble
97 E H -2.4529 Soluble
98 D H 0.0000 Buried
99 T H -0.7172 Soluble
100 A H 0.0000 Buried
101 V H 0.0491 Aggregation-prone
102 Y H 0.0000 Buried
103 H H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 R H -0.2866 Soluble
107 D H 0.0000 Buried
108 L H 1.4472 Aggregation-prone
109 V H 1.8065 Aggregation-prone
110 V H 2.4496 Aggregation-prone
113 Y H 2.1274 Aggregation-prone
114 G H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.6834 Soluble
117 V H -0.2972 Soluble
118 W H -0.6600 Soluble
119 G H 0.0000 Buried
120 Q H -1.3146 Soluble
121 G H 0.0000 Buried
122 T H -0.2495 Soluble
123 T H -0.0647 Soluble
124 V H 0.0000 Buried
125 T H -0.3901 Soluble
126 V H 0.0000 Buried
127 S H -0.7220 Soluble
128 S H -0.6700 Soluble
129 A H -0.5377 Soluble
130 S H -0.6111 Soluble
131 T H -1.1525 Soluble
132 K H -1.7639 Soluble
133 G H -1.7810 Soluble
134 P H 0.0000 Buried
135 S H -0.3983 Soluble
136 V H 0.0000 Buried
137 F H -0.7291 Soluble
138 P H -1.0657 Soluble
139 L H 0.0000 Buried
140 A H -0.8162 Soluble
141 P H 0.0000 Buried
142 S H -0.3678 Soluble
143 S H -0.2439 Soluble
144 G H -0.8382 Soluble
145 G H -0.8639 Soluble
146 T H -0.5293 Soluble
147 A H -0.2996 Soluble
148 A H 0.0000 Buried
149 L H 0.0000 Buried
150 G H 0.0000 Buried
151 C H 0.0000 Buried
152 L H 0.0000 Buried
153 V H 0.0000 Buried
154 K H 0.0000 Buried
155 D H -1.0683 Soluble
156 Y H 0.0000 Buried
157 F H 0.0000 Buried
158 P H -1.0806 Soluble
159 E H -1.5478 Soluble
160 P H -1.2772 Soluble
161 V H -0.8056 Soluble
162 T H -0.5800 Soluble
163 V H -0.1046 Soluble
164 S H -0.3456 Soluble
165 W H 0.0000 Buried
166 N H -0.6556 Soluble
167 S H -0.6216 Soluble
168 G H -0.5164 Soluble
169 A H -0.2064 Soluble
170 L H 0.0650 Aggregation-prone
171 T H -0.1432 Soluble
172 S H -0.1711 Soluble
173 G H -0.2141 Soluble
174 V H 0.2036 Aggregation-prone
175 H H -0.1407 Soluble
176 T H -0.0012 Soluble
177 F H 0.0000 Buried
178 P H -0.4620 Soluble
179 A H -0.0786 Soluble
180 V H 0.1576 Aggregation-prone
181 L H 0.3749 Aggregation-prone
182 Q H -0.2452 Soluble
183 S H -0.2762 Soluble
184 S H -0.4253 Soluble
185 G H -0.2786 Soluble
186 L H 0.0000 Buried
187 Y H 0.0000 Buried
188 S H 0.0000 Buried
189 L H 0.0000 Buried
190 S H 0.0000 Buried
191 S H 0.0000 Buried
192 V H 0.0000 Buried
193 V H 0.0000 Buried
194 T H -0.1198 Soluble
195 V H 0.0000 Buried
196 P H -0.6630 Soluble
197 S H -0.6702 Soluble
198 S H -0.7549 Soluble
199 S H -0.7804 Soluble
200 L H -0.9226 Soluble
201 G H -1.1170 Soluble
202 T H -0.8620 Soluble
203 Q H -1.4943 Soluble
204 T H -1.1751 Soluble
205 Y H 0.0000 Buried
206 I H -1.2108 Soluble
207 C H 0.0000 Buried
208 N H -1.4445 Soluble
209 V H 0.0000 Buried
210 N H -1.7566 Soluble
211 H H 0.0000 Buried
212 K H -2.8474 Soluble
213 P H -1.9154 Soluble
214 S H -2.0402 Soluble
215 N H -2.6007 Soluble
216 T H -2.1445 Soluble
217 K H -2.6985 Soluble
218 V H -1.7825 Soluble
219 D H -2.5194 Soluble
220 K H -2.1320 Soluble
221 R H -2.3352 Soluble
222 V H 0.0000 Buried
223 E H -2.4686 Soluble
224 P H -1.2481 Soluble
1 E L -1.4918 Soluble
2 I L 0.0069 Aggregation-prone
3 V L 1.0659 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.7875 Soluble
6 Q L 0.0000 Buried
7 S L -0.8321 Soluble
8 P L -0.4996 Soluble
9 G L -0.8088 Soluble
10 T L -0.4985 Soluble
11 L L -0.1321 Soluble
12 S L -0.4051 Soluble
13 L L -0.9500 Soluble
14 S L -1.4862 Soluble
15 P L -1.8547 Soluble
16 G L -2.3542 Soluble
17 E L -2.9785 Soluble
18 R L -3.1668 Soluble
19 A L 0.0000 Buried
20 T L -0.6584 Soluble
21 L L 0.0000 Buried
22 S L -0.8544 Soluble
23 C L 0.0000 Buried
24 R L -2.1286 Soluble
25 A L 0.0000 Buried
26 S L -0.7889 Soluble
27 Q L -1.0734 Soluble
28 S L -0.8882 Soluble
29 V L -0.2928 Soluble
30 S L -0.1970 Soluble
36 S L -0.0568 Soluble
37 S L 0.6710 Aggregation-prone
38 Y L 1.0166 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.4468 Soluble
46 P L -1.2151 Soluble
47 G L -1.3486 Soluble
48 Q L -1.7891 Soluble
49 A L -1.2575 Soluble
50 P L 0.0000 Buried
51 R L -1.3510 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.5640 Aggregation-prone
56 G L 0.5816 Aggregation-prone
57 A L -0.2189 Soluble
65 S L -0.6008 Soluble
66 S L -0.4684 Soluble
67 R L -1.3121 Soluble
68 A L -0.8269 Soluble
69 T L -0.5921 Soluble
70 G L -0.8001 Soluble
71 I L -0.9683 Soluble
72 P L -1.2444 Soluble
74 D L -2.1929 Soluble
75 R L -2.0249 Soluble
76 F L 0.0000 Buried
77 S L -0.8562 Soluble
78 G L -0.4806 Soluble
79 S L -0.5394 Soluble
80 G L -0.9932 Soluble
83 S L -0.8187 Soluble
84 G L -1.0288 Soluble
85 T L -1.4899 Soluble
86 D L -2.0213 Soluble
87 F L 0.0000 Buried
88 T L -0.6999 Soluble
89 L L 0.0000 Buried
90 T L -0.8295 Soluble
91 I L 0.0000 Buried
92 S L -2.3914 Soluble
93 R L -3.2246 Soluble
94 L L 0.0000 Buried
95 E L -1.8190 Soluble
96 P L -1.4683 Soluble
97 E L -2.0780 Soluble
98 D L 0.0000 Buried
99 F L -0.4438 Soluble
100 A L 0.0000 Buried
101 V L -0.0984 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 G L -0.6309 Soluble
114 S L -0.5946 Soluble
115 S L -0.3404 Soluble
116 P L 0.0000 Buried
117 T L -0.0113 Soluble
118 F L 0.0233 Aggregation-prone
119 G L 0.0000 Buried
120 Q L -1.7065 Soluble
121 G L -0.9804 Soluble
122 T L 0.0000 Buried
123 K L -0.4231 Soluble
124 L L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.4838 Soluble
127 K L -1.3781 Soluble
128 R L -1.0805 Soluble
129 T L -0.2612 Soluble
130 V L 0.4176 Aggregation-prone
131 A L 0.0892 Aggregation-prone
132 A L -0.1197 Soluble
133 P L 0.0000 Buried
134 S L -0.0776 Soluble
135 V L 0.0000 Buried
136 F L 0.1875 Aggregation-prone
137 I L 0.1493 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6236 Soluble
140 P L 0.0000 Buried
141 S L -1.6361 Soluble
142 D L -3.0233 Soluble
143 E L -3.2048 Soluble
144 Q L 0.0000 Buried
145 L L -2.1882 Soluble
146 K L -2.9010 Soluble
147 S L -1.7993 Soluble
148 G L -1.3133 Soluble
149 T L -1.0548 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9149 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.7755 Soluble
162 R L -3.0228 Soluble
163 E L -3.1493 Soluble
164 A L -2.3465 Soluble
165 K L -2.4296 Soluble
166 V L -1.1449 Soluble
167 Q L -0.4416 Soluble
168 W L 0.0000 Buried
169 K L -0.4341 Soluble
170 V L 0.0000 Buried
171 D L -1.6802 Soluble
172 N L -1.3170 Soluble
173 A L -0.2337 Soluble
174 L L 0.7658 Aggregation-prone
175 Q L -0.1581 Soluble
176 S L -0.3551 Soluble
177 G L -0.9518 Soluble
178 N L -0.9117 Soluble
179 S L -1.2151 Soluble
180 Q L -1.6007 Soluble
181 E L -1.5947 Soluble
182 S L -0.8685 Soluble
183 V L -0.7933 Soluble
184 T L -1.2113 Soluble
185 E L -2.2575 Soluble
186 Q L -1.8658 Soluble
187 D L -1.9874 Soluble
188 S L -1.6995 Soluble
189 K L -2.2816 Soluble
190 D L -1.7475 Soluble
191 S L -1.7239 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.6396 Soluble
199 L L 0.0000 Buried
200 T L -0.4693 Soluble
201 L L -0.6072 Soluble
202 S L -1.2161 Soluble
203 K L -2.1786 Soluble
204 A L -1.9030 Soluble
205 D L -2.4031 Soluble
206 Y L 0.0000 Buried
207 E L -3.6847 Soluble
208 K L -3.5764 Soluble
209 H L -2.9768 Soluble
210 K L -2.9353 Soluble
211 V L -1.2972 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6175 Soluble
216 V L 0.0000 Buried
217 T L -1.2022 Soluble
218 H L 0.0000 Buried
219 Q L -1.7603 Soluble
220 G L -0.4386 Soluble
221 L L -0.2291 Soluble
222 S L -0.4444 Soluble
223 S L -0.3885 Soluble
224 P L -0.5624 Soluble
225 V L 0.0536 Aggregation-prone
226 T L -0.3937 Soluble
227 K L -0.6563 Soluble
228 S L -0.4540 Soluble
229 F L -0.7672 Soluble
230 N L -1.7355 Soluble
231 R L -2.3435 Soluble
232 G L -1.9313 Soluble
233 E L -2.1317 Soluble
234 C L -0.4662 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
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pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.676 2.1749 CSV PDB
4.5 -0.7215 2.1749 CSV PDB
5.0 -0.7758 2.1749 CSV PDB
5.5 -0.829 2.1749 CSV PDB
6.0 -0.8701 2.1749 CSV PDB
6.5 -0.8896 2.1749 CSV PDB
7.0 -0.8866 2.1749 CSV PDB
7.5 -0.8686 2.1748 CSV PDB
8.0 -0.8422 2.1745 CSV PDB
8.5 -0.8098 2.1735 CSV PDB
9.0 -0.7713 2.1706 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH