Osocimab / 6hhc

A4DAB197_6hhc_H_L | Source PDB: 6hhc | Chains: H, L | Done
99.56
Yes
10 Å
Yes
Avg A4D Score
-0.7098
Max Score
1.9355
Min Score
-3.5586
Total Score
-303.1
Score CDR
-0.3014
Score non-CDR
-0.7604

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAASGFTFSQYGMDWVRQAPGKGLEWVSGIGPSGGSTVYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCTRGGPYYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKDIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYDASNLETGVPSRFSGSGSGTDFTFTISSLQPEDIATYYCQQANSFPVTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYSREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.5586
Maximum Score 1.9355
Average Score -0.7098
Total Score -303.1013

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.8136 Soluble
2 V H -0.6748 Soluble
3 Q H -0.8812 Soluble
4 L H 0.0000 Buried
5 L H 0.5983 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5479 Soluble
8 G H -0.8723 Soluble
9 G H -0.7583 Soluble
11 G H -0.4749 Soluble
12 L H -0.2670 Soluble
13 V H 0.0000 Buried
14 Q H -1.6384 Soluble
15 P H -1.5163 Soluble
16 G H -1.4465 Soluble
17 G H 0.0000 Buried
18 S H -1.1802 Soluble
19 L H -1.0608 Soluble
20 R H -2.1246 Soluble
21 L H 0.0000 Buried
22 S H -0.4552 Soluble
23 C H 0.0000 Buried
24 A H -0.1400 Soluble
25 A H 0.0000 Buried
26 S H -0.5907 Soluble
27 G H -0.9511 Soluble
28 F H -0.4685 Soluble
29 T H -0.4210 Soluble
30 F H 0.0000 Buried
35 S H -1.1790 Soluble
36 Q H -1.1865 Soluble
37 Y H -0.3825 Soluble
38 G H -0.4728 Soluble
39 M H 0.0000 Buried
40 D H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H 0.0000 Buried
45 A H -1.2467 Soluble
46 P H -1.3191 Soluble
47 G H -1.5548 Soluble
48 K H -2.2738 Soluble
49 G H -1.4822 Soluble
50 L H 0.0000 Buried
51 E H -1.3088 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 G H 0.0000 Buried
56 I H 0.0000 Buried
57 G H -0.6216 Soluble
58 P H -1.0137 Soluble
59 S H -1.0003 Soluble
62 G H -0.8661 Soluble
63 G H -0.7335 Soluble
64 S H -0.5126 Soluble
65 T H -0.1593 Soluble
66 V H 0.1279 Aggregation-prone
67 Y H -0.5496 Soluble
68 A H -1.1840 Soluble
69 D H -2.4676 Soluble
70 S H -1.8072 Soluble
71 V H 0.0000 Buried
72 K H -2.5843 Soluble
74 G H -1.8211 Soluble
75 R H -1.6327 Soluble
76 F H 0.0000 Buried
77 T H -0.8709 Soluble
78 I H 0.0000 Buried
79 S H -0.5236 Soluble
80 R H 0.0000 Buried
81 D H -1.8179 Soluble
82 N H -1.9436 Soluble
83 S H -1.6426 Soluble
84 K H -2.3758 Soluble
85 N H -1.6939 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.6393 Soluble
89 L H 0.0000 Buried
90 Q H -1.3215 Soluble
91 M H 0.0000 Buried
92 N H -1.5372 Soluble
93 S H -1.3029 Soluble
94 L H 0.0000 Buried
95 R H -2.3512 Soluble
96 A H -1.7240 Soluble
97 E H -2.2568 Soluble
98 D H 0.0000 Buried
99 T H -0.7591 Soluble
100 A H 0.0000 Buried
101 V H -0.1341 Soluble
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 T H 0.0000 Buried
106 R H -0.2439 Soluble
107 G H 0.0792 Aggregation-prone
108 G H 0.0000 Buried
109 P H 0.8681 Aggregation-prone
110 Y H 1.8802 Aggregation-prone
111 Y H 1.9355 Aggregation-prone
112 Y H 0.0000 Buried
113 Y H 0.7984 Aggregation-prone
114 G H 0.0000 Buried
115 M H 0.0000 Buried
116 D H -0.5241 Soluble
117 V H -0.3481 Soluble
118 W H -0.3353 Soluble
119 G H 0.0000 Buried
120 Q H -1.3593 Soluble
121 G H -0.5867 Soluble
122 T H -0.2389 Soluble
123 T H -0.1694 Soluble
124 V H 0.0000 Buried
125 T H -0.6058 Soluble
126 V H 0.0000 Buried
127 S H -0.7688 Soluble
128 S H -0.7345 Soluble
129 A H -0.4341 Soluble
130 S H -0.5821 Soluble
131 T H -1.0038 Soluble
132 K H -1.4642 Soluble
133 G H -1.5084 Soluble
134 P H 0.0000 Buried
135 S H -0.3814 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -0.9847 Soluble
139 L H 0.0000 Buried
140 A H -1.2059 Soluble
141 P H -0.8291 Soluble
142 G H -0.6289 Soluble
143 T H -0.4585 Soluble
144 A H -0.2408 Soluble
145 A H 0.0000 Buried
146 L H 0.0000 Buried
147 G H 0.0000 Buried
148 C H 0.0000 Buried
149 L H 0.0000 Buried
150 V H 0.0000 Buried
151 K H 0.0000 Buried
152 D H -0.7411 Soluble
153 Y H 0.0000 Buried
154 F H -0.4423 Soluble
155 P H 0.0000 Buried
156 E H -0.8456 Soluble
157 P H -0.8610 Soluble
158 V H -0.5099 Soluble
159 T H -0.5716 Soluble
160 V H -0.1466 Soluble
161 S H -0.3178 Soluble
162 W H 0.0000 Buried
163 N H -0.7216 Soluble
164 S H -0.6297 Soluble
165 G H -0.4919 Soluble
166 A H -0.2116 Soluble
167 L H 0.0518 Aggregation-prone
168 T H -0.1007 Soluble
169 S H -0.1452 Soluble
170 G H -0.1220 Soluble
171 V H 0.1954 Aggregation-prone
172 H H -0.3054 Soluble
173 T H 0.0398 Aggregation-prone
174 F H 0.0000 Buried
175 P H -0.4336 Soluble
176 A H 0.0511 Aggregation-prone
177 V H 0.1775 Aggregation-prone
178 L H 0.7099 Aggregation-prone
179 Q H 0.0188 Aggregation-prone
180 S H -0.3087 Soluble
181 S H -0.3580 Soluble
182 G H -0.1436 Soluble
183 L H -0.1552 Soluble
184 Y H 0.1198 Aggregation-prone
185 S H 0.0000 Buried
186 L H 0.0000 Buried
187 S H 0.0000 Buried
188 S H 0.0000 Buried
189 V H 0.0000 Buried
190 V H 0.0000 Buried
191 T H -0.1414 Soluble
192 V H 0.0000 Buried
193 P H -0.6016 Soluble
194 S H -0.5656 Soluble
195 S H -0.6569 Soluble
196 S H 0.0000 Buried
197 L H -0.8814 Soluble
198 G H -0.9889 Soluble
199 T H -0.8409 Soluble
200 Q H -1.4554 Soluble
201 T H -1.2206 Soluble
202 Y H 0.0000 Buried
203 I H -1.1283 Soluble
204 C H 0.0000 Buried
205 N H -1.3273 Soluble
206 V H 0.0000 Buried
207 N H -1.7763 Soluble
208 H H 0.0000 Buried
209 K H -2.6648 Soluble
210 P H -1.5753 Soluble
211 S H -1.8191 Soluble
212 N H -2.4516 Soluble
213 T H -2.2234 Soluble
214 K H -2.6137 Soluble
215 V H -1.4337 Soluble
216 D H -2.3656 Soluble
217 K H -1.8349 Soluble
218 K H -2.3817 Soluble
219 V H 0.0000 Buried
220 E H -2.8719 Soluble
221 P H -2.1032 Soluble
222 K H -2.5702 Soluble
1 D L -1.9826 Soluble
2 I L 0.0000 Buried
3 Q L -1.9570 Soluble
4 M L 0.0000 Buried
5 T L -1.1163 Soluble
6 Q L -0.7716 Soluble
7 S L -0.6614 Soluble
8 P L -0.5550 Soluble
9 S L -0.7508 Soluble
10 S L -0.8922 Soluble
11 L L -0.6515 Soluble
12 S L -0.9074 Soluble
13 A L -1.1348 Soluble
14 S L -1.0429 Soluble
15 V L -0.3562 Soluble
16 G L -0.9227 Soluble
17 D L -1.8307 Soluble
18 R L -2.3908 Soluble
19 V L 0.0000 Buried
20 T L -0.6342 Soluble
21 I L 0.0000 Buried
22 T L -0.7907 Soluble
23 C L 0.0000 Buried
24 Q L -2.2825 Soluble
25 A L 0.0000 Buried
26 S L -2.0411 Soluble
27 Q L -2.5623 Soluble
28 D L -2.6690 Soluble
29 I L 0.0000 Buried
36 S L -1.3160 Soluble
37 N L -1.1176 Soluble
38 Y L 0.1072 Aggregation-prone
39 L L 0.0000 Buried
40 N L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L -1.3009 Soluble
45 K L -1.7262 Soluble
46 P L -1.5886 Soluble
47 G L -1.8147 Soluble
48 K L -2.5169 Soluble
49 A L -1.6150 Soluble
50 P L 0.0000 Buried
51 K L -1.5247 Soluble
52 L L 0.0000 Buried
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.0000 Buried
56 D L -0.7959 Soluble
57 A L 0.0000 Buried
65 S L -1.0261 Soluble
66 N L -1.3686 Soluble
67 L L -0.4654 Soluble
68 E L -0.5442 Soluble
69 T L -0.4292 Soluble
70 G L -0.4695 Soluble
71 V L -0.1872 Soluble
72 P L -0.3282 Soluble
74 S L -0.4069 Soluble
75 R L -0.7998 Soluble
76 F L 0.0000 Buried
77 S L -0.5013 Soluble
78 G L -0.5716 Soluble
79 S L -1.0675 Soluble
80 G L -1.3584 Soluble
83 S L -1.4866 Soluble
84 G L -1.8171 Soluble
85 T L -2.2683 Soluble
86 D L -2.7420 Soluble
87 F L 0.0000 Buried
88 T L -0.7853 Soluble
89 F L 0.0000 Buried
90 T L -0.6359 Soluble
91 I L 0.0000 Buried
92 S L -1.4314 Soluble
93 S L -1.2444 Soluble
94 L L 0.0000 Buried
95 Q L -0.8795 Soluble
96 P L -1.5708 Soluble
97 E L -1.6414 Soluble
98 D L 0.0000 Buried
99 I L 0.0000 Buried
100 A L 0.0000 Buried
101 T L 0.0000 Buried
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 A L 0.2700 Aggregation-prone
108 N L -0.4223 Soluble
109 S L 0.1564 Aggregation-prone
114 F L 1.0521 Aggregation-prone
115 P L 0.2296 Aggregation-prone
116 V L 0.0000 Buried
117 T L -0.5485 Soluble
118 F L -0.4294 Soluble
119 G L 0.0000 Buried
120 G L -1.0467 Soluble
121 G L -0.8997 Soluble
122 T L 0.0000 Buried
123 K L -1.4098 Soluble
124 V L 0.0000 Buried
125 E L -1.6415 Soluble
126 I L 0.0000 Buried
127 K L -1.4193 Soluble
128 R L -1.0773 Soluble
129 T L -0.1954 Soluble
130 V L 0.1845 Aggregation-prone
131 A L -0.1064 Soluble
132 A L -0.0977 Soluble
133 P L 0.0000 Buried
134 S L 0.0526 Aggregation-prone
135 V L 0.2358 Aggregation-prone
136 F L 0.4485 Aggregation-prone
137 I L 0.2905 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6951 Soluble
140 P L 0.0000 Buried
141 S L -1.4067 Soluble
142 D L -2.1957 Soluble
143 E L -2.3117 Soluble
144 Q L 0.0000 Buried
145 L L -1.7156 Soluble
146 K L -2.5425 Soluble
147 S L -1.5749 Soluble
148 G L -1.3468 Soluble
149 T L -0.9388 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.9210 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 S L -1.8613 Soluble
162 R L -2.9438 Soluble
163 E L -3.2760 Soluble
164 A L -2.2836 Soluble
165 K L -2.1825 Soluble
166 V L -0.9379 Soluble
167 Q L -0.4527 Soluble
168 W L 0.0000 Buried
169 K L -0.5078 Soluble
170 V L 0.0000 Buried
171 D L -2.1277 Soluble
172 N L -1.5761 Soluble
173 A L -0.2671 Soluble
174 L L 0.7692 Aggregation-prone
175 Q L -0.2064 Soluble
176 S L -0.6243 Soluble
177 G L -1.2545 Soluble
178 N L -1.5264 Soluble
179 S L -1.4160 Soluble
180 Q L -1.5712 Soluble
181 E L -1.9085 Soluble
182 S L -1.0182 Soluble
183 V L -0.8621 Soluble
184 T L -1.1895 Soluble
185 E L -2.3736 Soluble
186 Q L 0.0000 Buried
187 D L -2.0405 Soluble
188 S L -2.2160 Soluble
189 K L -2.5479 Soluble
190 D L -1.9765 Soluble
191 S L 0.0000 Buried
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.7495 Soluble
199 L L 0.0000 Buried
200 T L -0.4806 Soluble
201 L L -0.7476 Soluble
202 S L -1.2135 Soluble
203 K L -1.9987 Soluble
204 A L -1.8560 Soluble
205 D L -2.7024 Soluble
206 Y L 0.0000 Buried
207 E L -3.2952 Soluble
208 K L -3.5586 Soluble
209 H L -3.1176 Soluble
210 K L -3.2212 Soluble
211 V L -1.6053 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.6454 Soluble
216 V L 0.0000 Buried
217 T L -1.2115 Soluble
218 H L 0.0000 Buried
219 Q L -1.8387 Soluble
220 G L -0.5034 Soluble
221 L L -0.2360 Soluble
222 S L -0.4520 Soluble
223 S L -0.4519 Soluble
224 P L -0.5871 Soluble
225 V L 0.0138 Aggregation-prone
226 T L -0.4051 Soluble
227 K L -0.6405 Soluble
228 S L -0.6072 Soluble
229 F L -1.2081 Soluble
230 N L -2.3880 Soluble
231 R L -2.4930 Soluble
232 G L -2.1011 Soluble
233 E L -2.6793 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6817 3.1232 CSV PDB
4.5 -0.7305 3.0605 CSV PDB
5.0 -0.7858 2.9911 CSV PDB
5.5 -0.8379 2.9192 CSV PDB
6.0 -0.8757 2.8466 CSV PDB
6.5 -0.8905 2.7739 CSV PDB
7.0 -0.8827 2.7015 CSV PDB
7.5 -0.8601 2.6305 CSV PDB
8.0 -0.8288 2.5636 CSV PDB
8.5 -0.7899 2.5066 CSV PDB
9.0 -0.7431 2.4676 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH