Amatuximab / 8cxc

A4DAB011_8cxc_H_L | Source PDB: 8cxc | Chains: H, L | Done
52.66
Yes
10 Å
Yes
Avg A4D Score
-0.6999
Max Score
2.0149
Min Score
-3.8685
Total Score
-300.27
Score CDR
0.0378
Score non-CDR
-0.7885

Calculation Parameters

Chain Sequence(s) EVQLLESGGGLVQPGGSLRLSCAASGLTFRSYAMTWVRQAPGKGLEWVSGISVSGGITYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKRGAAVGSFDYWGQGTLVTVSSAKTTAPSVYPLAPVTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGIQMTQSPSSLSASVGDRVTITCRSSQGIGSWLAWYQQKPEKAPQSLIYAASSLQSGVPSRFSGSGSGTDFTLTISNLQPEDFATYYCQQYNSYPLTFGGGTKVEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.8685
Maximum Score 2.0149
Average Score -0.6999
Total Score -300.2749

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 E H -1.9561 Soluble
2 V H -0.8273 Soluble
3 Q H -1.3217 Soluble
4 L H 0.0000 Buried
5 L H 0.1052 Aggregation-prone
6 E H 0.0000 Buried
7 S H -0.5170 Soluble
8 G H -1.0421 Soluble
9 G H -0.5407 Soluble
11 G H -0.2117 Soluble
12 L H -0.0190 Soluble
13 V H -0.7805 Soluble
14 Q H -1.8368 Soluble
15 P H -1.5838 Soluble
16 G H -1.4925 Soluble
17 G H -1.0446 Soluble
18 S H -1.0181 Soluble
19 L H -0.9854 Soluble
20 R H -1.9269 Soluble
21 L H 0.0000 Buried
22 S H -0.4930 Soluble
23 C H 0.0000 Buried
24 A H -0.3389 Soluble
25 A H -0.4654 Soluble
26 S H -0.8891 Soluble
27 G H -1.0115 Soluble
28 L H -0.4706 Soluble
29 T H -0.7014 Soluble
30 F H 0.0000 Buried
35 R H -1.8886 Soluble
36 S H -0.3773 Soluble
37 Y H 0.5512 Aggregation-prone
38 A H 0.0000 Buried
39 M H 0.0000 Buried
40 T H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H 0.0000 Buried
45 A H -1.0991 Soluble
46 P H -1.2235 Soluble
47 G H -1.7461 Soluble
48 K H -2.1047 Soluble
49 G H -1.3625 Soluble
50 L H -0.8583 Soluble
51 E H -0.9476 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 S H 0.0000 Buried
55 G H 0.0000 Buried
56 I H 0.0000 Buried
57 S H 1.4553 Aggregation-prone
58 V H 1.5638 Aggregation-prone
59 S H 0.5888 Aggregation-prone
62 G H 0.5278 Aggregation-prone
63 G H 0.6053 Aggregation-prone
64 I H 2.0149 Aggregation-prone
65 T H 1.1581 Aggregation-prone
66 Y H 0.7758 Aggregation-prone
67 Y H -0.4834 Soluble
68 A H -1.3099 Soluble
69 D H -2.5162 Soluble
70 S H -1.7748 Soluble
71 V H 0.0000 Buried
72 K H -2.5281 Soluble
74 G H -1.7009 Soluble
75 R H -1.4483 Soluble
76 F H 0.0000 Buried
77 T H -0.7665 Soluble
78 I H 0.0000 Buried
79 S H -0.4668 Soluble
80 R H -0.9093 Soluble
81 D H -2.1226 Soluble
82 N H -2.7800 Soluble
83 S H -2.0846 Soluble
84 K H -2.4725 Soluble
85 N H -2.1059 Soluble
86 T H 0.0000 Buried
87 L H 0.0000 Buried
88 Y H -0.4840 Soluble
89 L H 0.0000 Buried
90 Q H -1.1122 Soluble
91 M H 0.0000 Buried
92 N H -1.2816 Soluble
93 S H -1.1407 Soluble
94 L H 0.0000 Buried
95 R H -2.5687 Soluble
96 A H -1.8342 Soluble
97 E H -2.3174 Soluble
98 D H 0.0000 Buried
99 T H -0.7048 Soluble
100 A H 0.0000 Buried
101 V H 0.0621 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 K H 0.0000 Buried
107 R H 0.0000 Buried
108 G H 0.2627 Aggregation-prone
109 A H 0.3614 Aggregation-prone
110 A H 0.7491 Aggregation-prone
112 V H 0.5772 Aggregation-prone
113 G H 0.0000 Buried
114 S H 0.0000 Buried
115 F H 0.0000 Buried
116 D H 0.0713 Aggregation-prone
117 Y H 0.5068 Aggregation-prone
118 W H -0.0020 Soluble
119 G H 0.0000 Buried
120 Q H -1.7860 Soluble
121 G H -0.9095 Soluble
122 T H -0.0919 Soluble
123 L H 0.4700 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -1.3746 Soluble
128 S H -1.5492 Soluble
129 A H -1.6285 Soluble
130 K H -2.1050 Soluble
131 T H -0.8293 Soluble
132 T H -0.4916 Soluble
133 A H -0.4881 Soluble
134 P H -0.3152 Soluble
135 S H -0.2393 Soluble
136 V H 0.0000 Buried
137 Y H -0.5149 Soluble
138 P H -0.8535 Soluble
139 L H 0.0000 Buried
140 A H -0.2853 Soluble
141 P H 0.5341 Aggregation-prone
142 V H 0.9513 Aggregation-prone
143 T H -0.1366 Soluble
144 T H -0.4114 Soluble
145 G H -0.6696 Soluble
146 S H -0.5804 Soluble
147 S H -0.3734 Soluble
148 V H -0.1065 Soluble
149 T H 0.0000 Buried
150 L H 0.0000 Buried
151 G H 0.0000 Buried
152 C H 0.0000 Buried
153 L H 0.0000 Buried
154 V H 0.0000 Buried
155 K H -0.6637 Soluble
156 G H -0.5249 Soluble
157 Y H 0.0000 Buried
158 F H 0.0000 Buried
159 P H -0.5872 Soluble
160 E H -0.6669 Soluble
161 P H -0.6373 Soluble
162 V H 0.0000 Buried
163 T H -0.4037 Soluble
164 L H -0.0784 Soluble
165 T H -0.2903 Soluble
166 W H 0.0000 Buried
167 N H -1.0997 Soluble
168 S H -0.8857 Soluble
169 G H -0.7705 Soluble
170 S H -0.5505 Soluble
171 L H -0.1446 Soluble
172 S H -0.3321 Soluble
173 S H -0.1834 Soluble
174 G H 0.0046 Aggregation-prone
175 V H 0.2426 Aggregation-prone
176 H H -0.0369 Soluble
177 T H 0.0448 Aggregation-prone
178 F H 0.0000 Buried
179 P H -0.1825 Soluble
180 A H 0.2286 Aggregation-prone
181 V H 1.0334 Aggregation-prone
182 L H 1.0981 Aggregation-prone
183 Q H -0.1858 Soluble
184 S H -0.8704 Soluble
185 D H -1.8098 Soluble
186 L H -0.5592 Soluble
187 Y H 0.2080 Aggregation-prone
188 T H 0.2235 Aggregation-prone
189 L H 0.0000 Buried
190 S H 0.0000 Buried
191 S H 0.0000 Buried
192 S H 0.0000 Buried
193 V H 0.0000 Buried
194 T H 0.1038 Aggregation-prone
195 V H 0.1619 Aggregation-prone
196 T H -0.2176 Soluble
197 S H -0.4144 Soluble
198 S H -0.4962 Soluble
199 T H -0.5329 Soluble
200 W H -0.5783 Soluble
201 P H -1.0339 Soluble
202 S H -0.9703 Soluble
203 Q H -1.4709 Soluble
204 S H -1.4162 Soluble
205 I H 0.0000 Buried
206 T H -1.7002 Soluble
207 C H 0.0000 Buried
208 N H -1.5170 Soluble
209 V H 0.0000 Buried
210 A H -0.9755 Soluble
211 H H 0.0000 Buried
212 P H -0.7467 Soluble
213 A H -0.3415 Soluble
214 S H -0.4800 Soluble
215 S H -0.6570 Soluble
216 T H -0.9841 Soluble
217 K H -1.9013 Soluble
218 V H -1.1213 Soluble
219 D H -2.5168 Soluble
220 K H -2.0364 Soluble
221 K H -2.6513 Soluble
222 I H 0.0000 Buried
223 E H -2.7908 Soluble
224 P H -1.8264 Soluble
225 R H -2.3500 Soluble
226 G H -1.3982 Soluble
2 I L 0.3137 Aggregation-prone
3 Q L -1.1639 Soluble
4 M L 0.0000 Buried
5 T L -1.3878 Soluble
6 Q L -0.9011 Soluble
7 S L -0.7167 Soluble
8 P L -0.5252 Soluble
9 S L -0.6239 Soluble
10 S L -0.6774 Soluble
11 L L -0.2074 Soluble
12 S L -0.6472 Soluble
13 A L -1.0293 Soluble
14 S L -1.3639 Soluble
15 V L -0.5240 Soluble
16 G L -1.9544 Soluble
17 D L -3.1183 Soluble
18 R L -3.1609 Soluble
19 V L 0.0000 Buried
20 T L -0.7471 Soluble
21 I L 0.0000 Buried
22 T L -0.9153 Soluble
23 C L 0.0000 Buried
24 R L -2.9592 Soluble
25 S L -1.9600 Soluble
26 S L -1.5246 Soluble
27 Q L -1.7427 Soluble
28 G L -1.6223 Soluble
29 I L 0.0000 Buried
36 G L -0.9055 Soluble
37 S L -0.3507 Soluble
38 W L 0.1721 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L -0.9018 Soluble
44 Q L 0.0000 Buried
45 K L -2.9998 Soluble
46 P L -2.5957 Soluble
47 E L -3.3081 Soluble
48 K L -3.4499 Soluble
49 A L -2.1119 Soluble
50 P L 0.0000 Buried
51 Q L -1.3095 Soluble
52 S L -0.2623 Soluble
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L 0.7975 Aggregation-prone
56 A L 0.4338 Aggregation-prone
57 A L -0.0602 Soluble
65 S L -0.1639 Soluble
66 S L 0.2691 Aggregation-prone
67 L L 0.5263 Aggregation-prone
68 Q L 0.0763 Aggregation-prone
69 S L -0.2671 Soluble
70 G L -0.4541 Soluble
71 V L -0.2123 Soluble
72 P L -0.3608 Soluble
74 S L -0.4201 Soluble
75 R L -1.1495 Soluble
76 F L 0.0000 Buried
77 S L -0.3401 Soluble
78 G L 0.0000 Buried
79 S L -0.7118 Soluble
80 G L -1.2600 Soluble
83 S L -1.0189 Soluble
84 G L -1.4095 Soluble
85 T L -2.0171 Soluble
86 D L -2.4301 Soluble
87 F L 0.0000 Buried
88 T L -0.8729 Soluble
89 L L 0.0000 Buried
90 T L -0.7502 Soluble
91 I L 0.0000 Buried
92 S L -2.0337 Soluble
93 N L -2.7364 Soluble
94 L L 0.0000 Buried
95 Q L -1.5675 Soluble
96 P L -1.2339 Soluble
97 E L -2.2034 Soluble
98 D L 0.0000 Buried
99 F L -0.6911 Soluble
100 A L -1.0183 Soluble
101 T L -0.8776 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 Y L 0.0000 Buried
108 N L -0.6572 Soluble
109 S L -0.1072 Soluble
114 Y L 0.3797 Aggregation-prone
115 P L 0.0264 Aggregation-prone
116 L L 0.0000 Buried
117 T L -0.2075 Soluble
118 F L -0.3878 Soluble
119 G L -0.8073 Soluble
120 G L -0.9643 Soluble
121 G L 0.0000 Buried
122 T L 0.0000 Buried
123 K L -1.1274 Soluble
124 V L 0.0000 Buried
125 E L -0.7804 Soluble
126 I L -0.6647 Soluble
127 K L -2.1712 Soluble
128 R L -2.3262 Soluble
129 A L -1.7117 Soluble
130 D L -2.4748 Soluble
131 A L -1.2641 Soluble
132 A L -0.5946 Soluble
133 P L -0.0257 Soluble
134 T L -0.0770 Soluble
135 V L 0.0000 Buried
136 S L -0.0521 Soluble
137 I L 0.0000 Buried
138 F L 0.0000 Buried
139 P L 0.1217 Aggregation-prone
140 P L -0.3047 Soluble
141 S L -0.6065 Soluble
142 S L -0.8765 Soluble
143 E L -1.1769 Soluble
144 Q L 0.0000 Buried
145 L L -0.8070 Soluble
146 T L -0.6751 Soluble
147 S L -0.8153 Soluble
148 G L -1.1097 Soluble
149 G L -0.9097 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 F L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8115 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -2.2845 Soluble
162 K L -2.4017 Soluble
163 D L -2.3225 Soluble
164 I L -1.6088 Soluble
165 N L -1.7051 Soluble
166 V L -0.6045 Soluble
167 K L -1.7100 Soluble
168 W L 0.0000 Buried
169 K L -2.3405 Soluble
170 I L 0.0000 Buried
171 D L -2.7435 Soluble
172 G L -2.0038 Soluble
173 S L -2.0466 Soluble
174 E L -3.2245 Soluble
175 R L -2.6226 Soluble
176 Q L -2.5719 Soluble
177 N L -2.1431 Soluble
178 G L -0.9545 Soluble
179 V L -0.6073 Soluble
180 L L -0.0936 Soluble
181 N L -0.3548 Soluble
182 S L -0.2095 Soluble
183 W L -0.4714 Soluble
184 T L -1.0257 Soluble
185 D L -2.1220 Soluble
186 Q L -1.9793 Soluble
187 D L -2.2781 Soluble
188 S L -1.8666 Soluble
189 K L -2.4477 Soluble
190 D L -2.0686 Soluble
191 S L -2.0829 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 M L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L 0.0000 Buried
199 L L 0.0000 Buried
200 T L -0.2082 Soluble
201 L L 0.0000 Buried
202 T L -1.4280 Soluble
203 K L -2.3596 Soluble
204 D L -3.4248 Soluble
205 E L -2.9593 Soluble
206 Y L 0.0000 Buried
207 E L -3.6894 Soluble
208 R L -3.8685 Soluble
209 H L -3.2139 Soluble
210 N L -2.8081 Soluble
211 S L -2.1798 Soluble
212 Y L 0.0000 Buried
213 T L -0.8430 Soluble
214 C L 0.0000 Buried
215 E L -0.2854 Soluble
216 A L 0.0000 Buried
217 T L -0.7780 Soluble
218 H L 0.0000 Buried
219 K L -2.3450 Soluble
220 T L -1.3219 Soluble
221 S L -0.6426 Soluble
222 T L -0.5325 Soluble
223 S L -0.2519 Soluble
224 P L -0.0916 Soluble
225 I L 1.0254 Aggregation-prone
226 V L 1.1652 Aggregation-prone
227 K L 0.0012 Aggregation-prone
228 S L -0.2410 Soluble
229 F L 0.0000 Buried
230 N L -2.0508 Soluble
231 R L -2.1987 Soluble
232 N L -2.8182 Soluble
233 E L -2.6409 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
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Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
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A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.6843 2.9853 CSV PDB
4.5 -0.7297 2.9853 CSV PDB
5.0 -0.783 2.9853 CSV PDB
5.5 -0.835 2.9853 CSV PDB
6.0 -0.8751 2.9853 CSV PDB
6.5 -0.8949 2.9853 CSV PDB
7.0 -0.8945 2.9853 CSV PDB
7.5 -0.8809 2.9853 CSV PDB
8.0 -0.8598 2.9853 CSV PDB
8.5 -0.8322 2.9852 CSV PDB
9.0 -0.7972 2.9849 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH