Nivolumab / 5wt9

A4DAB182_5wt9_H_L | Source PDB: 5wt9 | Chains: H, L | Done
100
Yes
10 Å
Yes
Avg A4D Score
-0.7834
Max Score
1.0542
Min Score
-3.6816
Total Score
-329.82
Score CDR
-0.7754
Score non-CDR
-0.7843

Calculation Parameters

Chain Sequence(s) QVQLVESGGGVVQPGRSLRLDCKASGITFSNSGMHWVRQAPGKGLEWVAVIWYDGSKRYYADSVKGRFTISRDNSKNTLFLQMNSLRAEDTAVYYCATNDDYWGQGTLVTVSSASTKGPSVFPLAPCSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKEIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQSSNWPRTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE input.pdb
Selected Chain(s) H (Heavy), L (Light)
Distance of Aggregation 10 Å
FoldX Energy Minimization Yes
pH Calculations Yes
Downloads Download all data (.tar.gz)

Aggrescan4D Profile

Aggregation-prone (score > 0)
Soluble (score < 0)
Buried (score = 0)

Per-Residue A4D Scores

Download CSV
Minimum Score -3.6816
Maximum Score 1.0542
Average Score -0.7834
Total Score -329.8194

Residues with A4D score > 0.0 are highlighted as aggregation-prone.

Index Residue Chain A4D Score Classification
1 Q H -1.1268 Soluble
2 V H -0.1659 Soluble
3 Q H -1.1889 Soluble
4 L H 0.0000 Buried
5 V H -0.4291 Soluble
6 E H 0.0000 Buried
7 S H -0.5199 Soluble
8 G H -0.6262 Soluble
9 G H -0.2928 Soluble
11 G H 0.1998 Aggregation-prone
12 V H 0.4894 Aggregation-prone
13 V H 0.0000 Buried
14 Q H -1.9405 Soluble
15 P H -2.1410 Soluble
16 G H -2.1819 Soluble
17 R H -2.7983 Soluble
18 S H -1.9899 Soluble
19 L H -1.1905 Soluble
20 R H -1.5191 Soluble
21 L H 0.0000 Buried
22 D H -0.8104 Soluble
23 C H 0.0000 Buried
24 K H -1.5595 Soluble
25 A H 0.0000 Buried
26 S H -0.8590 Soluble
27 G H -0.5813 Soluble
28 I H -0.2562 Soluble
29 T H -0.5848 Soluble
30 F H 0.0000 Buried
35 S H -1.4120 Soluble
36 N H -1.4707 Soluble
37 S H -1.2333 Soluble
38 G H -1.0517 Soluble
39 M H 0.0000 Buried
40 H H 0.0000 Buried
41 W H 0.0000 Buried
42 V H 0.0000 Buried
43 R H 0.0000 Buried
44 Q H -0.7881 Soluble
45 A H -1.1689 Soluble
46 P H -0.9381 Soluble
47 G H -1.5167 Soluble
48 K H -2.3914 Soluble
49 G H -1.6090 Soluble
50 L H 0.0000 Buried
51 E H -1.1515 Soluble
52 W H 0.0000 Buried
53 V H 0.0000 Buried
54 A H 0.0000 Buried
55 V H 0.0000 Buried
56 I H 0.0000 Buried
57 W H -0.9623 Soluble
58 Y H -0.7917 Soluble
59 D H -2.0460 Soluble
62 G H -1.5157 Soluble
63 S H -1.3879 Soluble
64 K H -1.7034 Soluble
65 R H -1.3357 Soluble
66 Y H -0.5992 Soluble
67 Y H -0.9546 Soluble
68 A H 0.0000 Buried
69 D H -2.6308 Soluble
70 S H -1.8235 Soluble
71 V H 0.0000 Buried
72 K H -2.5467 Soluble
74 G H -1.7751 Soluble
75 R H -1.3982 Soluble
76 F H 0.0000 Buried
77 T H -0.9181 Soluble
78 I H 0.0000 Buried
79 S H -0.4967 Soluble
80 R H -1.1561 Soluble
81 D H -1.9387 Soluble
82 N H -2.2066 Soluble
83 S H -1.7746 Soluble
84 K H -2.6802 Soluble
85 N H -2.1010 Soluble
86 T H -1.6222 Soluble
87 L H 0.0000 Buried
88 F H -0.4407 Soluble
89 L H 0.0000 Buried
90 Q H -1.1438 Soluble
91 M H 0.0000 Buried
92 N H -1.7730 Soluble
93 S H -1.7183 Soluble
94 L H 0.0000 Buried
95 R H -2.5586 Soluble
96 A H -1.7355 Soluble
97 E H -2.2415 Soluble
98 D H 0.0000 Buried
99 T H -0.6374 Soluble
100 A H 0.0000 Buried
101 V H 0.2573 Aggregation-prone
102 Y H 0.0000 Buried
103 Y H 0.0000 Buried
104 C H 0.0000 Buried
105 A H 0.0000 Buried
106 T H 0.0000 Buried
107 N H -1.8751 Soluble
115 D H -2.1824 Soluble
116 D H -1.2800 Soluble
117 Y H -0.4394 Soluble
118 W H -0.5119 Soluble
119 G H 0.0000 Buried
120 Q H -1.4564 Soluble
121 G H -0.6387 Soluble
122 T H -0.0854 Soluble
123 L H 0.4385 Aggregation-prone
124 V H 0.0000 Buried
125 T H 0.0000 Buried
126 V H 0.0000 Buried
127 S H -0.8458 Soluble
128 S H -0.5794 Soluble
129 A H -0.4839 Soluble
130 S H -0.6047 Soluble
131 T H -0.7806 Soluble
132 K H -1.6724 Soluble
133 G H -1.6114 Soluble
134 P H -0.7463 Soluble
135 S H -0.5094 Soluble
136 V H 0.0000 Buried
137 F H 0.0000 Buried
138 P H -1.2385 Soluble
139 L H 0.0000 Buried
140 A H -1.0294 Soluble
141 P H 0.0000 Buried
142 C H 0.2978 Aggregation-prone
143 S H -0.3635 Soluble
144 T H -0.2307 Soluble
145 A H 0.0000 Buried
146 A H 0.0000 Buried
147 L H 0.0000 Buried
148 G H 0.0000 Buried
149 C H 0.0000 Buried
150 L H 0.0000 Buried
151 V H 0.0000 Buried
152 K H 0.0000 Buried
153 D H -0.6387 Soluble
154 Y H 0.0000 Buried
155 F H 0.0000 Buried
156 P H 0.0000 Buried
157 E H -0.4423 Soluble
158 P H -0.7691 Soluble
159 V H -0.5586 Soluble
160 T H -0.6097 Soluble
161 V H -0.2101 Soluble
162 S H -0.5002 Soluble
163 W H 0.0000 Buried
164 N H -0.9715 Soluble
165 S H -0.7686 Soluble
166 G H -0.5913 Soluble
167 A H -0.2573 Soluble
168 L H -0.1118 Soluble
169 T H -0.2086 Soluble
170 S H -0.4383 Soluble
171 G H -0.3906 Soluble
172 V H 0.1645 Aggregation-prone
173 H H 0.0000 Buried
174 T H -0.1153 Soluble
175 F H 0.0000 Buried
176 P H -0.4777 Soluble
177 A H 0.0762 Aggregation-prone
178 V H 0.3293 Aggregation-prone
179 L H 1.0542 Aggregation-prone
180 Q H 0.2479 Aggregation-prone
181 S H -0.0427 Soluble
182 S H -0.2025 Soluble
183 G H 0.0112 Aggregation-prone
184 L H -0.0302 Soluble
185 Y H 0.2406 Aggregation-prone
186 S H 0.0000 Buried
187 L H 0.0000 Buried
188 S H 0.0000 Buried
189 S H 0.0000 Buried
190 V H 0.0000 Buried
191 V H 0.0000 Buried
192 T H -0.1679 Soluble
193 V H 0.0000 Buried
194 P H -0.5088 Soluble
195 S H -0.2575 Soluble
196 S H -0.5526 Soluble
197 S H -0.6599 Soluble
198 L H -0.6827 Soluble
199 G H -1.0042 Soluble
200 T H -0.9036 Soluble
201 K H -1.6825 Soluble
202 T H -1.4531 Soluble
203 Y H 0.0000 Buried
204 T H -1.8383 Soluble
205 C H 0.0000 Buried
206 N H -1.7271 Soluble
207 V H 0.0000 Buried
208 D H -1.9874 Soluble
209 H H 0.0000 Buried
210 K H -2.7565 Soluble
211 P H -1.7490 Soluble
212 S H -1.9861 Soluble
213 N H -2.6171 Soluble
214 T H -2.1686 Soluble
215 K H -2.7299 Soluble
216 V H -1.8930 Soluble
217 D H -2.8083 Soluble
218 K H -2.3908 Soluble
219 R H -2.9505 Soluble
220 V H 0.0000 Buried
221 E H -2.8527 Soluble
222 S H -1.7262 Soluble
223 K H -2.3060 Soluble
1 E L -1.8555 Soluble
2 I L 0.0000 Buried
3 V L 0.4531 Aggregation-prone
4 L L 0.0000 Buried
5 T L -0.7517 Soluble
6 Q L 0.0000 Buried
7 S L -0.7396 Soluble
8 P L -0.2818 Soluble
9 A L -0.3259 Soluble
10 T L -0.1349 Soluble
11 L L -0.0557 Soluble
12 S L -0.4632 Soluble
13 L L -0.8582 Soluble
14 S L -1.1770 Soluble
15 P L -1.5557 Soluble
16 G L -1.7170 Soluble
17 E L -2.1777 Soluble
18 R L -2.5789 Soluble
19 A L 0.0000 Buried
20 T L -0.6532 Soluble
21 L L 0.0000 Buried
22 S L -0.9472 Soluble
23 C L 0.0000 Buried
24 R L -2.4150 Soluble
25 A L 0.0000 Buried
26 S L -1.0864 Soluble
27 Q L -1.6973 Soluble
28 S L -1.2543 Soluble
29 V L 0.0000 Buried
36 S L -0.5689 Soluble
37 S L -0.3588 Soluble
38 Y L 0.2929 Aggregation-prone
39 L L 0.0000 Buried
40 A L 0.0000 Buried
41 W L 0.0000 Buried
42 Y L 0.0000 Buried
43 Q L 0.0000 Buried
44 Q L 0.0000 Buried
45 K L -1.4023 Soluble
46 P L -1.3927 Soluble
47 G L -1.4806 Soluble
48 Q L -2.1228 Soluble
49 A L -1.3788 Soluble
50 P L 0.0000 Buried
51 R L -1.2292 Soluble
52 L L -0.5934 Soluble
53 L L 0.0000 Buried
54 I L 0.0000 Buried
55 Y L -0.3706 Soluble
56 D L -0.8787 Soluble
57 A L 0.0000 Buried
65 S L -1.0384 Soluble
66 N L -1.4451 Soluble
67 R L -1.6214 Soluble
68 A L -1.1750 Soluble
69 T L -0.6418 Soluble
70 G L -0.7143 Soluble
71 I L 0.0000 Buried
72 P L -0.5019 Soluble
74 A L -0.4312 Soluble
75 R L -0.8455 Soluble
76 F L 0.0000 Buried
77 S L -0.7344 Soluble
78 G L -0.7432 Soluble
79 S L -0.9005 Soluble
80 G L -1.2146 Soluble
83 S L -1.0743 Soluble
84 G L -1.1095 Soluble
85 T L -1.8166 Soluble
86 D L -2.3178 Soluble
87 F L 0.0000 Buried
88 T L -0.8240 Soluble
89 L L 0.0000 Buried
90 T L -0.6159 Soluble
91 I L 0.0000 Buried
92 S L -1.3284 Soluble
93 S L -1.6941 Soluble
94 L L 0.0000 Buried
95 E L -2.1826 Soluble
96 P L -1.6192 Soluble
97 E L -2.2638 Soluble
98 D L 0.0000 Buried
99 F L -0.5153 Soluble
100 A L -0.3428 Soluble
101 V L -0.0304 Soluble
102 Y L 0.0000 Buried
103 Y L 0.0000 Buried
104 C L 0.0000 Buried
105 Q L 0.0000 Buried
106 Q L 0.0000 Buried
107 S L -0.1090 Soluble
108 S L -0.3494 Soluble
109 N L -0.8757 Soluble
114 W L -0.4527 Soluble
115 P L -0.8068 Soluble
116 R L -0.4493 Soluble
117 T L -0.3600 Soluble
118 F L -0.0802 Soluble
119 G L 0.0000 Buried
120 Q L -1.4020 Soluble
121 G L -0.8868 Soluble
122 T L 0.0000 Buried
123 K L -0.3833 Soluble
124 V L 0.0000 Buried
125 E L 0.0000 Buried
126 I L -0.5163 Soluble
127 K L -1.1971 Soluble
128 R L -0.8806 Soluble
129 T L -0.1070 Soluble
130 V L 0.3104 Aggregation-prone
131 A L -0.0558 Soluble
132 A L -0.1423 Soluble
133 P L 0.0000 Buried
134 S L -0.0879 Soluble
135 V L 0.0000 Buried
136 F L 0.2319 Aggregation-prone
137 I L 0.2251 Aggregation-prone
138 F L 0.0000 Buried
139 P L -0.6507 Soluble
140 P L -0.8019 Soluble
141 S L -1.7919 Soluble
142 D L -3.0529 Soluble
143 E L -2.8607 Soluble
144 Q L 0.0000 Buried
145 L L -2.0502 Soluble
146 K L -2.7975 Soluble
147 S L -1.7847 Soluble
148 G L -1.1414 Soluble
149 T L -1.1000 Soluble
150 A L 0.0000 Buried
151 S L 0.0000 Buried
152 V L 0.0000 Buried
153 V L 0.0000 Buried
154 C L 0.0000 Buried
155 L L 0.0000 Buried
156 L L 0.0000 Buried
157 N L 0.0000 Buried
158 N L -0.8439 Soluble
159 F L 0.0000 Buried
160 Y L 0.0000 Buried
161 P L -1.6795 Soluble
162 R L -2.9101 Soluble
163 E L -3.1431 Soluble
164 A L -2.2299 Soluble
165 K L -2.3411 Soluble
166 V L -1.0621 Soluble
167 Q L -0.6179 Soluble
168 W L 0.0000 Buried
169 K L -0.5684 Soluble
170 V L 0.0000 Buried
171 D L -1.9767 Soluble
172 N L -1.3840 Soluble
173 A L -0.2772 Soluble
174 L L 0.6671 Aggregation-prone
175 Q L -0.3030 Soluble
176 S L -0.6867 Soluble
177 G L -1.3555 Soluble
178 N L -1.7392 Soluble
179 S L -1.6180 Soluble
180 Q L -1.6496 Soluble
181 E L -1.9926 Soluble
182 S L -0.9011 Soluble
183 V L -0.8805 Soluble
184 T L -1.1871 Soluble
185 E L -2.2310 Soluble
186 Q L -1.7690 Soluble
187 D L -1.7976 Soluble
188 S L -1.6339 Soluble
189 K L -2.1857 Soluble
190 D L -1.5613 Soluble
191 S L -1.5426 Soluble
192 T L 0.0000 Buried
193 Y L 0.0000 Buried
194 S L 0.0000 Buried
195 L L 0.0000 Buried
196 S L 0.0000 Buried
197 S L 0.0000 Buried
198 T L -0.8167 Soluble
199 L L 0.0000 Buried
200 T L -0.6846 Soluble
201 L L -0.9279 Soluble
202 S L -1.1709 Soluble
203 K L -2.1684 Soluble
204 A L -2.0259 Soluble
205 D L -2.8773 Soluble
206 Y L 0.0000 Buried
207 E L -3.5462 Soluble
208 K L -3.6816 Soluble
209 H L -2.9185 Soluble
210 K L -2.7296 Soluble
211 V L -1.2357 Soluble
212 Y L 0.0000 Buried
213 A L 0.0000 Buried
214 C L 0.0000 Buried
215 E L -0.8618 Soluble
216 V L 0.0000 Buried
217 T L -1.2082 Soluble
218 H L 0.0000 Buried
219 Q L -1.7407 Soluble
220 G L -0.4932 Soluble
221 L L -0.2639 Soluble
222 S L -0.4837 Soluble
223 S L -0.4241 Soluble
224 P L -0.5443 Soluble
225 V L 0.0148 Aggregation-prone
226 T L -0.4953 Soluble
227 K L -0.6785 Soluble
228 S L -0.5632 Soluble
229 F L -0.9541 Soluble
230 N L -1.9622 Soluble
231 R L -2.3685 Soluble
232 G L -2.0514 Soluble
233 E L -2.4897 Soluble

3D Structure — colored by A4D score

Sequence - click residue to focus in viewer  |  colored by A4D score
Load structure to view sequence…
Downloads
Color Legend (B-factor = A4D Score)
Score > 1.5 — highly aggregation-prone
Score 0.5–1.5 — aggregation-prone
Score ≈ 0 — buried
Score −1 to −0.5 — soluble
Score < −2.5 — highly soluble
pH Structure
Load structure at specific pH condition
A4D Score Threshold
Highlight residues > threshold in the sequence panel
CDR Regions
HCDR1
HCDR2
HCDR3
LCDR1
LCDR2
LCDR3
Display Options

pH-Dependent Aggregation Profiles

A4D scores calculated across 11 pH conditions (4.0–9.0, step 0.5). Click a row to view the structure at that pH. The input structure is globular — max score is recommended for pH analysis.

pH Avg A4D Score Max A4D Score Downloads
4.0 -0.7866 1.778 CSV PDB
4.5 -0.8367 1.7161 CSV PDB
5.0 -0.8947 1.6224 CSV PDB
5.5 -0.9507 1.512 CSV PDB
6.0 -0.9934 1.4025 CSV PDB
6.5 -1.014 1.3497 CSV PDB
7.0 -1.0121 1.3687 CSV PDB
7.5 -0.9943 1.4118 CSV PDB
8.0 -0.9666 1.5277 CSV PDB
8.5 -0.9306 1.6848 CSV PDB
9.0 -0.8862 1.8426 CSV PDB
Average A4D Score vs pH
Max A4D Score vs pH